Abstract
Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and TTG agars were the best selective media, whereas sucrose tellurite teepol agar was the poorest. Both TTG and sucrose tellurite teepol agars were good for use in follow-up serological tests, whereas only TTG agar could be used for follow-up oxidase tests. In our opinion TTG agar has more advantages for cholera research laboratories routinely culturing large numbers of patients for cholera on a daily basis and where media needs can be accurately predicted. In contrast, in smaller clinical laboratories or in laboratories investigating epidemics, thiosulfate citrate bile salts sucrose agar is best because it is commercially available and easy to prepare and can be used to distinguish colonies of suspect V. cholerae from V. parahaemolyticus.
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- COCHRAN W. G. The comparison of percentages in matched samples. Biometrika. 1950 Dec;37(3-4):256–266. [PubMed] [Google Scholar]
- Chatterjee B. D., De P. K., Sen T. Sucrose teepol tellurite agar: a new selective indicator medium for isolation of Vibrio species. J Infect Dis. 1977 Apr;135(4):654–658. doi: 10.1093/infdis/135.4.654. [DOI] [PubMed] [Google Scholar]
- De S. P., Sen D., De P. C., Ghosh A., Pal S. C. A simple selective medium for isolation of vibrios with particular reference to vibrio parahaemolyticus. Indian J Med Res. 1977 Sep;66(3):398–399. [PubMed] [Google Scholar]
- Gangarosa E. J., Bennett J. V., Boring J. R., 3rd Differentiation between vibrio cholerae and Vibrio cholerae biotype El Tor by the polymyxin B disc test: comparative results with TCBS, Monsur's, Mueller-Hinton and nutrient agar media. Bull World Health Organ. 1967;36(6):987–990. [PMC free article] [PubMed] [Google Scholar]
- Gangarosa E. J., Dewitt W. E., Huq I., Zarifi A. Laboratory methods in cholera: isolation of Vibrio cholerae (el tor and classical) on TCBS medium in minimally equipped laboratories. Trans R Soc Trop Med Hyg. 1968;62(5):693–699. doi: 10.1016/0035-9203(68)90121-1. [DOI] [PubMed] [Google Scholar]
- KOBAYASHI T., ENOMOTO S., SAKAZAKI R., KUWAHARA S. [A NEW SELECTIVE ISOLATION MEDIUM FOR THE VIBRIO GROUP; ON A MODIFIED NAKANISHI'S MEDIUM (TCBS AGAR MEDIUM)]. Nihon Saikingaku Zasshi. 1963 Nov;18:387–392. doi: 10.3412/jsb.18.387. [DOI] [PubMed] [Google Scholar]
- KOVACS N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature. 1956 Sep 29;178(4535):703–703. doi: 10.1038/178703a0. [DOI] [PubMed] [Google Scholar]
- MONSUR K. A. A highly selective gelatin-taurocholate-tellurite medium for the isolation of Vibrio cholerae. Trans R Soc Trop Med Hyg. 1961 Sep;55:440–442. doi: 10.1016/0035-9203(61)90090-6. [DOI] [PubMed] [Google Scholar]
- MUKERJEE S. Diagnostic uses of cholera bacteriophages. J Hyg (Lond) 1961 Mar;59:109–115. doi: 10.1017/s0022172400038754. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCormack W. M., DeWitt W. E., Bailey P. E., Morris G. K., Soeharjono P., Gangarosa E. J. Evaluation of thiosulfate-citrate-bile salts-sucrose agar, a selective medium for the isolation of Vibrio cholerae and other pathogenic vibrios. J Infect Dis. 1974 May;129(5):497–500. doi: 10.1093/infdis/129.5.497. [DOI] [PubMed] [Google Scholar]
- Merson M. H., Martin W. T., Craig J. P., Morris G. K., Blake P. A., Craun G. F., Feeley J. C., Camacho J. C., Gangarosa E. J. Cholera on Guam, 1974: epidemiologic findings and isolation of non-toxinogenic strains. Am J Epidemiol. 1977 Apr;105(4):349–361. doi: 10.1093/oxfordjournals.aje.a112393. [DOI] [PubMed] [Google Scholar]