Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Jan;9(1):84–87. doi: 10.1128/jcm.9.1.84-87.1979

Recovery of Haemophilus influenzae from twenty-three blood culture media.

R F Schell, J L Le Frock, J P Babu, D B Robinson
PMCID: PMC272961  PMID: 311778

Abstract

Haemophilus influenzae is an important agent of bacteremia and has fastidious growth requirements. The purpose of this investigation was to determine the ability of commercial blood culture media to support the growth of this fastidious microorganism. Twenty-three types of blood culture media were inoculated with individual suspensions of eight strains of H. influenzae in the presence or absence of an erythrocyte-serum mixture. The rates of recovery of the H. influenzae strains from the various types of blood culture media were compared. The results demonstrated that the type of medium, the manufacturer, the erythrocyte-serum mixture, and the strain of H. influenzae influenced the recovery rates of H. influenzae. Optimal recovery of the strains of H. influenzae was obtained from brain heart infustion blood culture medium (GIBCO). Trypic soy broth (GIBCO) and supplemental peptone of Becton, Dickinson and Co. also were found to be superior to the remaining types of media tested for the recovery of H. influenzae.

Full text

PDF
84

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babu J. P., Schell R. F., Le Frock J. L. Evaluation of twenty-three blood culture media. J Clin Microbiol. 1978 Sep;8(3):288–292. doi: 10.1128/jcm.8.3.288-292.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Drapkin M. S., Wilson M. E., Shrager S. M., Rubin R. H. Bacteremic hemophilus influenzae type B cellulitis in the adult. Am J Med. 1977 Sep;63(3):449–452. doi: 10.1016/0002-9343(77)90284-4. [DOI] [PubMed] [Google Scholar]
  3. Geraci J. E., Wilkowske C. J., Wilson W. R., Washington J. A., 2nd Haemophilus endocarditis. Report of 14 patients. Mayo Clin Proc. 1977 Apr;52(4):209–215. [PubMed] [Google Scholar]
  4. Harlow M., Chung S. M., Plotkin S. A. Hemophilus influenzae septic arthritis in infants and children. Clin Pediatr (Phila) 1975 Dec;14(12):1146–1149. doi: 10.1177/000992287501401207. [DOI] [PubMed] [Google Scholar]
  5. Hodge J. L., Bremner D. A. Haemophilus endocarditis and the isolation of haemophilic bacteria in blood culture: case report. N Z Med J. 1974 Apr 10;79(512):824–825. [PubMed] [Google Scholar]
  6. Johnson R. H., Kennedy R. P., Marton K. I., Thornsberry C. Hemophilus endocarditis: new cases, literature review and recommendations for management. South Med J. 1977 Sep;70(9):1098–1102. [PubMed] [Google Scholar]
  7. Larson S. M., Charache P., Chen M., Wagner H. N., Jr Automated detection of Haemophilus influenzae. Appl Microbiol. 1973 Jun;25(6):1011–1012. doi: 10.1128/am.25.6.1011-1012.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levin D. C., Schwarz M. I., Matthay R. A., LaForce F. M. Bacteremic hemophilus influenzae pneumonia in adults. A report of 24 cases and a review of the literature. Am J Med. 1977 Feb;62(2):219–224. doi: 10.1016/0002-9343(77)90317-5. [DOI] [PubMed] [Google Scholar]
  9. McGowan J. E., Jr, Bratton L., Klein J. O., Finland M. Bacteremia in febrile children seen in a "walk-in" pediatric clinic. N Engl J Med. 1973 Jun 21;288(25):1309–1312. doi: 10.1056/NEJM197306212882501. [DOI] [PubMed] [Google Scholar]
  10. McGowan J. E., Jr, Klein J. O., Bratton L., Barnes M. W., Finland M. Meningitis and bacteremia due to Haemophilus influenzae: occurrence and mortality at Boston City Hospital in 12 selected years, 1935-1972. J Infect Dis. 1974 Aug;130(2):119–124. doi: 10.1093/infdis/130.2.119. [DOI] [PubMed] [Google Scholar]
  11. Peter G., Smith D. H. Hemophilus influenzae meningitis at the Children's Hospital Medical Center in Boston, 1958 to 1973. Pediatrics. 1975 Apr;55(4):523–526. [PubMed] [Google Scholar]
  12. Quintiliani R., Hymans P. J. The association of bacteremic Haemophilus influenzae pneumonia in adults with typable strains. Am J Med. 1971 Jun;50(6):781–786. doi: 10.1016/0002-9343(71)90186-0. [DOI] [PubMed] [Google Scholar]
  13. Raff M. J., Dannaher C. L. Hemophilus influenzae septic arthritis in adults. Report of a case and review of the literature. J Bone Joint Surg Am. 1974 Mar;56(2):408–412. [PubMed] [Google Scholar]
  14. Sell S. H. The clinical importance of Hemophilus influenzae infections in children. Pediatr Clin North Am. 1970 May;17(2):415–426. doi: 10.1016/s0031-3955(16)32419-1. [DOI] [PubMed] [Google Scholar]
  15. Thiemke W. A., Wicher K. Laboratory experience with a radiometric method for detecting bacteremia. J Clin Microbiol. 1975 Mar;1(3):302–308. doi: 10.1128/jcm.1.3.302-308.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tillotson J. R., Lerner A. M. Hemophilus influenzae bronchopneumonia in adults. Arch Intern Med. 1968 May;121(5):428–432. [PubMed] [Google Scholar]
  17. Washington J. A., 2nd Blood cultures: principles and techniques. Mayo Clin Proc. 1975 Feb;50(2):91–98. [PubMed] [Google Scholar]
  18. Washington J. A., 2nd Comparison of two commercially available media for detection of bacteremia. Appl Microbiol. 1971 Oct;22(4):604–607. doi: 10.1128/am.22.4.604-607.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Washington J. A., 2nd Evaluation of two commercially available media for detection of bacteremia. Appl Microbiol. 1972 May;23(5):956–959. doi: 10.1128/am.23.5.956-959.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weinstein L. Type b haemophilus influenzae infections in adults. N Engl J Med. 1970 Jan 22;282(4):221–222. doi: 10.1056/NEJM197001222820411. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES