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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Jan;9(1):97–102. doi: 10.1128/jcm.9.1.97-102.1979

Electron capture gas-liquid chromatographic-mass spectral identification of acids produced by Neisseria meningitidis in a defined medium.

C C Alley, J B Brooks, D S Kellogg Jr
PMCID: PMC272964  PMID: 107187

Abstract

The acid metabolites and the cellular fatty acids of three strains of Neisseria meningitidis grown in a chemically defined liquid medium were determined with computerized frequency-pulsed electron capture gas-liquid chromatography. Five acids not previously reported were subsequently identified: isobutyric, octanoic, decenoic (C10:1), dodecenoic (C12:1), and tetradecenoic (C14:1). These acids were produced during active metabolism and were not detected as cellular constituents. The frequency-pulsed electron capture gas-liquid chromatography methods which we used provide a rapid, reliable, sensitive means of detecting both these and other metabolic and cellular acids in spent culture medium.

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Selected References

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