Abstract
Immunodiffusion techniques were used on trichloroacetic acid extracts from 10 strains of Bacteroides fragilis in detecting precipitating antibodies against this species in immune rabbit sera. Species and even strain specificities were observed in these precipitin reactions. Multiple antigens were detected in the extracts from some strains, whereas only one precipitin band per extract developed during agar-gel diffusion tests of others. The antigen extracts were found to be both heat stable and resistant to hydrolysis by alpha-chymotrypsin. Four serological patterns were demonstrated in homologous and heterologous reactions with the B. fragilis. antigen-antibody systems used. The results showed that some strains were serologically distinct from others, indicating that the strains tested are of more than one serotype.
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- Abshire R. L., Lombard G. L., Dowell V. R., Jr Fluorescent-antibody studies on selected strains of Bacteroides fragilis subspecies fragilis. J Clin Microbiol. 1977 Oct;6(4):425–432. doi: 10.1128/jcm.6.4.425-432.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BUCK A. A., MAYER H. COMPARATIVE STUDIES OF THE RAPID PLASMA REAGIN CARD TEST FOR SYPHILIS AND THE VDRL SLIDE TEST IN ETHIOPIA. Am J Hyg. 1964 Jul;80:85–90. doi: 10.1093/oxfordjournals.aje.a120462. [DOI] [PubMed] [Google Scholar]
- Beerens H., Wattre P., Shinjo T., Romond C. Premiers résultats d'un essai de classification sérologique de 131 souches de Bacteroïdes du groupe fragilis (eggerthella. Ann Inst Pasteur (Paris) 1971 Aug;121(2):187–198. [PubMed] [Google Scholar]
- Cummins C. S. Identification of Propionibacterium acnes and related organisms by precipitin tests with trichloroacetic acid extracts. J Clin Microbiol. 1976 Aug;2(2):104–110. [PMC free article] [PubMed] [Google Scholar]
- Danielsson D., Lambe D. W., Jr, Persson S. The immune response in a patient to an infection with Bacteroides fragilis ss. fragilis and Clostridium difficile. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(5):709–712. doi: 10.1111/j.1699-0463.1972.tb00198.x. [DOI] [PubMed] [Google Scholar]
- De la Cruz E., Cuadra C. Antigenic characteristics of five species of human Bacteroides. J Bacteriol. 1969 Nov;100(2):1116–1117. doi: 10.1128/jb.100.2.1116-1117.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Felner J. M., Dowell V. R., Jr "Bacteroides" bacteremia. Am J Med. 1971 Jun;50(6):787–796. doi: 10.1016/0002-9343(71)90187-2. [DOI] [PubMed] [Google Scholar]
- Finegold S. M. Infections due to anaerobes. Med Times. 1968 Feb;96(2):174–187. [PubMed] [Google Scholar]
- Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
- Hofstad T. Cross-reactivity of Bacteroides fragilis O antigens. Acta Pathol Microbiol Scand B. 1977 Feb;85B(1):9–13. doi: 10.1111/j.1699-0463.1977.tb01668.x. [DOI] [PubMed] [Google Scholar]
- Hofstad T., Kristoffersen T. Chemical characteristics of endotoxin from Bacteroides fragilis NCTC 9343. J Gen Microbiol. 1970 Apr;61(1):15–19. doi: 10.1099/00221287-61-1-15. [DOI] [PubMed] [Google Scholar]
- Kasper D. L. The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. J Infect Dis. 1976 Jan;133(1):79–87. doi: 10.1093/infdis/133.1.79. [DOI] [PubMed] [Google Scholar]
- Lambe D. W., Jr, Danielsson D., Vroon D. H., Carver R. K. Immune responses in eight patients infected with Bacteroides fragilis. J Infect Dis. 1975 May;131(5):499–508. doi: 10.1093/infdis/131.5.499. [DOI] [PubMed] [Google Scholar]
- Lambe D. W., Jr, Moroz D. A. Serogrouping of Bacteroides fragilis subsp. fragilis by the agglutination test. J Clin Microbiol. 1976 Jun;3(6):586–592. doi: 10.1128/jcm.3.6.586-592.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leigh D. A. Clinical importance of infections due to Bacteroides fragilis and role of antibiotic therapy. Br Med J. 1974 Jul 27;3(5925):225–228. doi: 10.1136/bmj.3.5925.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reinhold L. Serologische Untersuchungen an Stämmen von Bacteroides thetaiotaomicron und Bacteroides fragilis im Agargelpräzipitationstest. Zentralbl Bakteriol Orig. 1971;216(2):219–227. [PubMed] [Google Scholar]
- Rissing J. P., Crowder J. G., Smith J. W., White A. Detection of Bacteroides fragilis infection by precipitin antibody. J Infect Dis. 1974 Jul;130(1):70–73. doi: 10.1093/infdis/130.1.70. [DOI] [PubMed] [Google Scholar]
- Romond C., Beerens H., Wattre P. Identification sérologique des bactéroïdes en relation avec leur pouvoir pathogène. Arch Roum Pathol Exp Microbiol. 1972 Sep;31(3):351–355. [PubMed] [Google Scholar]
- Shinjo T., Beerens H., Wattre P., Romond C. Classification sérologique de 131 souches de bacteroides du groupe fragilis (Eggerthella. Ann Inst Pasteur Lille. 1971;22:85–100. [PubMed] [Google Scholar]
- Sonnenwirth A. C., Yin E. T., Sarmiento E. M., Wessler S. Bacterioidaceae endotoxin detection by Limulus assay. Am J Clin Nutr. 1972 Dec;25(12):1452–1454. doi: 10.1093/ajcn/25.12.1452. [DOI] [PubMed] [Google Scholar]
- Stauffer L. R., Hill E. O., Holland J. W., Altemeier W. A. Indirect fluorescent antibody procedure for the rapid detection and identification of Bacteroides and Fusobacterium in clinical specimens. J Clin Microbiol. 1975 Oct;2(4):337–344. doi: 10.1128/jcm.2.4.337-344.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson W. R., Martin W. J., Wilkowske C. J., Washington J. A., 2nd Anaerobic bacteremia. Mayo Clin Proc. 1972 Sep;47(9):639–646. [PubMed] [Google Scholar]
- Zabransky R. J. Isolation of anaerobic bacteria from clinical specimens. Mayo Clin Proc. 1970 Apr;45(4):256–264. [PubMed] [Google Scholar]