Representative Western Blot of total and phosphorylated eNOS in human coronary artery endothelial cells exposed to isoflurane (APC, 0.42 mM or 1 Minimal Alveolar Concentration) compared to control (CON) experiments in the absence of volatile anesthetic with or without pre-treatment with geldanamycin (GELD, 17.8 μM), radicicol (RAD, 20 μM) or N-G-mono-methyl-L-arginine monoacetate (L-NMMA, 1mM). Histograms show the ratio of phospho- to total-eNOS in each group.
Data are mean±SD; *: P<0.05) vs CON. n=6 per group.