Table 2.

Kinetic parameters of the FRET peptide substrate of the central region of synaptobrevin: the substrate of BoNT/B LC produced by our laboratory (11), the Roques laboratory (12) (Anne, et al., 2001) and the native substrate (syb 60–94) (Shone and Roberts, 1994).

Substrate	KM(μM)	kcat(s−1)	kcat/KM (s−1M−1)
11a	63	0.17	2.7 × 103
12b	47	45	9.6 × 105
Nativeb	330	24	7.3 × 104

^akinetic experiments of the substrate were performed at 24°C

^bkinetic experiments of the substrate were performed at 37°C.