Abstract
An enzyme immunoassay (EIA) was developed for detecting Brucella antibodies in milk of cows infected with Brucella abortus. The enzyme immunoassay using heat-killed cells of B. abortus strain 19 was of comparable sensitivity to the Brucella ring test in detecting antibodies in milk of a reference positive control cow experimentally infected with B. abortus strain 2308 and in milk of 16 naturally infected cows from which B. abortus was isolated. No detectable enzyme immunoassay reactions were present in milk of 11 noninfected controls. The enzyme immunoassay is a procedure which can be readily automated so that screening tests for brucellosis could be conducted at a reference laboratory where uniform conditions can be maintained.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Kaneene J. M., Johnson D. W., Anderson R. K., Angus R. D., Pietz D. E., Muscoplat C. C. Kinetics of in vitro bovine lymphocyte immunostimulation with a Brucella abortus antigen. Am J Vet Res. 1978 Feb;39(2):235–239. [PubMed] [Google Scholar]
- Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
- PIETZ D. E., ANDERSON R. K., WERRING D. F. EFFECT OF TIME AND STORAGE TEMPERATURE OF MILK ON THE BRUCELLA MILK RING TEST. J Am Vet Med Assoc. 1963 Dec 1;143:1202–1207. [PubMed] [Google Scholar]
- Roepke M. H., Patterson J. M., Deyoe B. L. Brucella ring test sensitivity of individual and pooled bovine milks with various preservatives. Am J Vet Res. 1974 Jan;35(1):115–118. [PubMed] [Google Scholar]
- Saunders G. C., Clinard E. H. Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test. J Clin Microbiol. 1976 Jun;3(6):604–608. doi: 10.1128/jcm.3.6.604-608.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thoen C. O., Armbrust A. L., Eacret W. G., Harrington R., Jr, Brown G. M. Use of an enzyme immunoassay test for characterizing the A and M antigens of Brucella. J Clin Microbiol. 1979 Apr;9(4):485–487. doi: 10.1128/jcm.9.4.485-487.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thoen C. O., Eacret W. G., Himes E. M. An enzyme-labeled antibody test for detecting antibodies in chickens infected with Mycobacterium avium serotype 2. Avian Dis. 1978 Jan-Mar;22(1):162–166. [PubMed] [Google Scholar]