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. 1979 Aug;10(2):235–239. doi: 10.1128/jcm.10.2.235-239.1979

Effects of cell culture and laboratory conditions on type 2 dengue virus infectivity.

J S Manning, J K Collins
PMCID: PMC273136  PMID: 41848

Abstract

The stability of type 2 dengue virus to exposure to a variety of laboratory conditions was determined. Suckling mouse brain passage virus was adapted for growth in BHK-21 cells, and plaque assays were performed using a tragacanth gum overlay. A three- to fourfold increase in plaque size could be obtained if monolayers were subconfluent at time of inoculation. Incubation of virus for 24 h at 37 degrees C, pH 6.5, or in buffer containing 1 mM ethylenediaminetetraacetate considerably reduced virus infectivity as compared with virus incubated for the same period at 4 degrees C, pH 8.0, or in buffer with or without 1 mM CaCl2 and 1 mM MgCl2. Multiple freezing and thawing of virus tissue culture medium containing 10% fetal calf serum did not reduce virus infectivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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