Abstract
A counterimmunoelectroosmophoresis (CIE) technique is described for the detection of calf diarrhea coronavirus antigens in intestinal contents. The antibody reagent was prepared in rabbits against the Nebraska calf diarrhea coronavirus adapted to Vero cells and purified by density gradient centrifugation. The method was applied to intestinal contents of diarrheic and normal calves and compared with electron microscopy (EM). Calf coronavirus antigens were detected in intestinal contents of 44% (21/48) of the diarrheic calves and 24% (4/17) of the normal calves. Two precipitin lines could be observed in the majority of the positive samples. When compared with EM, CIE detected more positive animals. In only two cases (2/20) CIE was negative despite the visualization of coronavirus particles by EM.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bohac J., Derbyshire J. B. The demonstration of transmissible gastroenteritis viral antigens by immunoelectrophoresis and counterimmunoelectrophoresis. Can J Microbiol. 1975 Jun;21(6):750–753. doi: 10.1139/m75-111. [DOI] [PubMed] [Google Scholar]
- Bohac J., Derbyshire J. B., Thorsen J. The detection of transmissible gastroenteritis viral antigens by immunodiffusion. Can J Comp Med. 1975 Jan;39(1):67–75. [PMC free article] [PubMed] [Google Scholar]
- Chasey D., Lucas M. A bovine coronavirus identified by thin-section electron microscopy. Vet Rec. 1977 Jun 18;100(25):530–531. doi: 10.1136/vr.100.25.530. [DOI] [PubMed] [Google Scholar]
- House J. A. Economic impact of rotavirus and other neonatal disease agents of animals. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 2):573–576. [PubMed] [Google Scholar]
- Inaba Y., Sato K., Kurogi H., Takahashi E., Ito Y. Replication of bovine coronavirus in cell line BEK-1 culture. Arch Virol. 1976;50(4):339–342. doi: 10.1007/BF01317959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marsolais G., Assaf R., Montpetit C., Marois P. Diagnosis of viral agents associated with neonatal calf diarrhea. Can J Comp Med. 1978 Apr;42(2):168–171. [PMC free article] [PubMed] [Google Scholar]
- Mebus C. A., Newman L. E., Stair E. L., Jr Scanning electron, light, and immunofluorescent microscopy of intestine of gnotobiotic calf infected with calf diarrheal coronavirus. Am J Vet Res. 1975 Dec;36(12):1719–1725. [PubMed] [Google Scholar]
- Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. Neonatal calf diarrhea: propagation, attenuation, and characteristics of a coronavirus-like agent. Am J Vet Res. 1973 Feb;34(2):145–150. [PubMed] [Google Scholar]
- Mengeling W. L. Precipitating antigens of hemagglutinating encephalomyelitis virus demonstrated by agar gel immunodiffusion. Am J Vet Res. 1972 Jul;33(7):1527–1535. [PubMed] [Google Scholar]
- Morin M., Lamothe P., Gagnon A., Malo R. A case of viral neonatal calf diarrhea in a Quebec dairy herd. Can J Comp Med. 1974 Jul;38(3):236–242. [PMC free article] [PubMed] [Google Scholar]
- Scherrer R., Cohen J., L'Haridon R., Feynerol C., Fayet J. C. Reovirus-like agent (rotavirus) associated with neonatal calf gastroenteritis in France. Ann Rech Vet. 1976;7(1):25–31. [PubMed] [Google Scholar]
- Sharpee R. L., Mebus C. A., Bass E. P. Characterization of a calf diarrheal coronavirus. Am J Vet Res. 1976 Sep;37(9):1031–1041. [PubMed] [Google Scholar]
- Stair E. L., Rhodes M. B., White R. G., Mebus C. A. Neonatal calf diarrhea: purification and electron microscopy of a coronavirus-like agent. Am J Vet Res. 1972 Jun;33(6):1147–1156. [PubMed] [Google Scholar]
- Tevethia S. S., Cunningham C. H. Antigenic characterization of infectious bronchitis virus. J Immunol. 1968 Apr;100(4):793–798. [PubMed] [Google Scholar]
- Woode G. N., Bridger J. C., Hall G., Dennis M. J. The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Res Vet Sci. 1974 Jan;16(1):102–105. [PubMed] [Google Scholar]