Abstract
A rapid, sensitive counterimmunoelectrophoresis assay was developed to detect adenovirus in stools of patients with gastroenteritis. The parameters of the assay were optimized, and its sensitivity and efficiency was examined. The assay promises to be a useful alternative to conventional techniques of cell culture isolation or electron microscopy for the detection of adenovirus in stool specimens.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLISON A. C., PEREIRA H. G., FARTHING C. P. Investigation of adenovirus antigens by agar gel diffusion techniques. Virology. 1960 Mar;10:316–328. doi: 10.1016/0042-6822(60)90023-4. [DOI] [PubMed] [Google Scholar]
- BRADSTREET C. M., TAYLOR C. E. Technique of complementfixation test applicable to the diagnosis of virus diseases. Mon Bull Minist Health Public Health Lab Serv. 1962 May;21:96–104. [PubMed] [Google Scholar]
- DUNCAN I. B., HUTCHISON J. G. Type-3 adenovirus infection with gastrointestinal symptoms. Lancet. 1961 Mar 11;1(7176):530–532. doi: 10.1016/s0140-6736(61)91599-9. [DOI] [PubMed] [Google Scholar]
- Fulton R. E., Middleton P. J. Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children. Infect Immun. 1974 Jul;10(1):92–101. doi: 10.1128/iai.10.1.92-101.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREEN M., PINA M. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963 May;20:199–207. doi: 10.1016/0042-6822(63)90157-0. [DOI] [PubMed] [Google Scholar]
- Hierholzer J. C., Barme M. Counterimmunoelectrophoresis with adenovirus type-specific anti-hemagglutinin sera as a rapid diagnostic method. J Immunol. 1974 Mar;112(3):987–995. [PubMed] [Google Scholar]
- Middleton P. J., Petric M., Hewitt C. M., Szymanski M. T., Tam J. S. Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody. J Clin Pathol. 1976 Mar;29(3):191–197. doi: 10.1136/jcp.29.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Am J Dis Child. 1977 Jul;131(7):733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
- Moffet H. L., Shulenberger H. K., Burkholder E. R. Epidemiology and etiology of severe infantile diarrhea. J Pediatr. 1968 Jan;72(1):1–14. doi: 10.1016/s0022-3476(68)80394-4. [DOI] [PubMed] [Google Scholar]
- Petric M., Middleton P. J., Mankikar S. D. Detection of adenovirus antibody by counterimmunoelectrophoresis. J Clin Microbiol. 1979 Aug;10(2):256–258. doi: 10.1128/jcm.10.2.256-258.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YIN-COGGRAVE M. Identification of adenoviruses by microprecipitin agar-gel diffusion. Lancet. 1962 Jun 16;1(7242):1273–1275. doi: 10.1016/s0140-6736(62)92368-1. [DOI] [PubMed] [Google Scholar]