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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Sep;10(3):275–278. doi: 10.1128/jcm.10.3.275-278.1979

Biotyping of Escherichia coli by a simple multiple-inoculation agar plate technique.

F J Buckwold, A R Ronald, G K Harding, T J Marrie, L Fox, C Cates
PMCID: PMC273151  PMID: 385615

Abstract

A nine-test system using multiple-inoculation agar plates for biotyping of Escherichia coli is described. Testing of 959 strains resulted in 78 biotypes. On repeated testing, 96% of 182 strains had identical biotypes or differed by only one test. This system provides satisfactory differentiation among strains and is reproducible. Precise standardization of inoculum size is not required. Multiple inoculation allows time and cost-efficient testing of large numbers of strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butler D. A., Lobregat C. M., Gavan T. L. Reproducibility of the analytab (API 20E) system. J Clin Microbiol. 1975 Oct;2(4):322–326. doi: 10.1128/jcm.2.4.322-326.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davies B. I. Biochemical typing of urinary Escherichia coli strains by means of the API 20 E enterobacteriaceae system. J Med Microbiol. 1977 Aug;10(3):293–298. doi: 10.1099/00222615-10-3-293. [DOI] [PubMed] [Google Scholar]
  3. MCGEACHIE J. SEROLOGICAL GROUPING OF URINARY ESCHERICHIA COLI. J Clin Pathol. 1965 Jul;18:428–431. doi: 10.1136/jcp.18.4.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mabeck C. E., Orskov F., Orskov I. Studies in urinary tract infections. 8. Escherichia coli O:H serotypes in recurrent infections. Acta Med Scand. 1971 Oct;190(4):279–282. doi: 10.1111/j.0954-6820.1971.tb07430.x. [DOI] [PubMed] [Google Scholar]
  5. Murray P. R. Standardization of the Analytab Enteric (API 20E) system to increase accuracy and reproducibility of the test for biotype characterization of bacteria. J Clin Microbiol. 1978 Jul;8(1):46–49. doi: 10.1128/jcm.8.1.46-49.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. TURCK M., PETERSDORF R. G. The epidemiology of nonenteric Escherichia coli infections: prevalence of serological groups. J Clin Invest. 1962 Sep;41:1760–1765. doi: 10.1172/JCI104635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Van Der Waaij D., Speltie T. M., Guinee P. A., Agterberg C. Serotyping and biotyping of 160 Escherichia coli strains: comparative study. J Clin Microbiol. 1975 Feb;1(2):237–238. doi: 10.1128/jcm.1.2.237-238.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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