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. 1979 Sep;10(3):279–284. doi: 10.1128/jcm.10.3.279-284.1979

Demonstration of antibodies against Yersinia enterocolitica lipopolysaccharide in human sera by enzyme-linked immunosorbent assay.

M Gripenberg, A Nissinen, E Väisänen, E Linder
PMCID: PMC273152  PMID: 489721

Abstract

Antibodies against Yersinia enterocolitica serotype O:3 lipopolysaccharide present in sera from patients with Yersinia infection were studied by using an enzyme-linked immunosorbent assay. Of the sera with significant bacterial agglutination titers against Y. enterocolitica type O:3, 86% contained anti-lipopolysaccharide antibodies of the immunoglobulin G class. With the sera of some patients, we demonstrated increasing anti-lipopolysaccharide antibody levels of immunoglobulin G class in spite of decreasing bacterial agglutination titers. The assay was specific for lipopolysaccharide from Y. enterocolitica type O:3, and in inhibition experiments lipopolysaccharide could be detected in amounts of greater than or equal to 0.5 micrograms/ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahvonen P. Human yersiniosis in Finland. I. Bacteriology and serology. Ann Clin Res. 1972 Feb;4(1):30–38. [PubMed] [Google Scholar]
  2. Ahvonen P. Human yersiniosis in Finland. II. Clinical features. Ann Clin Res. 1972 Feb;4(1):39–48. [PubMed] [Google Scholar]
  3. Arvastson B., Damgaard K., Winblad S. Clinical symptoms of infection with Yersinia enterocolitica. Scand J Infect Dis. 1971;3(1):37–40. doi: 10.3109/inf.1971.3.issue-1.06. [DOI] [PubMed] [Google Scholar]
  4. Butler J. E., Feldbush T. L., McGivern P. L., Stewart N. The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity. Immunochemistry. 1978 Feb;15(2):131–136. doi: 10.1016/0161-5890(78)90053-6. [DOI] [PubMed] [Google Scholar]
  5. Carlsson H. E., Hurvell B., Lindberg A. A. Enzyme-linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica. Acta Pathol Microbiol Scand C. 1976 Jun;84(3):168–176. doi: 10.1111/j.1699-0463.1976.tb00016.x. [DOI] [PubMed] [Google Scholar]
  6. Carlsson H. E., Lindberg A. A., Hammarström S., Ljunggren A. Quantitation of Salmonella O-antibodies in human sera by enzyme-linked immunosorbent assay (ELISA). Int Arch Allergy Appl Immunol. 1975;48(4):485–494. doi: 10.1159/000231336. [DOI] [PubMed] [Google Scholar]
  7. Carlsson H. E., Lindberg A. A., Hammarström S. Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay. Infect Immun. 1972 Nov;6(5):703–708. doi: 10.1128/iai.6.5.703-708.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Engvall E., Jonsson K., Perlmann P. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim Biophys Acta. 1971 Dec 28;251(3):427–434. doi: 10.1016/0005-2795(71)90132-2. [DOI] [PubMed] [Google Scholar]
  9. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  10. Gripenberg M., Linder E., Kurki P., Engvall E. A solid phase enzyme-linked immunosurbent assay (ELISA) for the demonstration of antibodies against denatured, single-stranded DNA in patient sera. Scand J Immunol. 1978;7(2):151–157. doi: 10.1111/j.1365-3083.1978.tb00438.x. [DOI] [PubMed] [Google Scholar]
  11. Holmgren J., Svennerholm A. M. Enzyme-linked immunosorbent assays for cholera serology. Infect Immun. 1973 May;7(5):759–763. doi: 10.1128/iai.7.5.759-763.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Laitinen O., Leirisalo M., Skylv G. Relation between HLA-B27 and clinical features in patients with yersinia arthritis. Arthritis Rheum. 1977 Jun;20(5):1121–1124. doi: 10.1002/art.1780200512. [DOI] [PubMed] [Google Scholar]
  14. Levin J., Poore T. E., Zauber N. P., Oser R. S. Detection of endotoxin in the blood of patients with sepsis due to gran-negative bacteria. N Engl J Med. 1970 Dec 10;283(24):1313–1316. doi: 10.1056/NEJM197012102832404. [DOI] [PubMed] [Google Scholar]
  15. McDevitt H. O., Bodmer W. F. HL-A, immune-response genes, and disease. Lancet. 1974 Jun 22;1(7869):1269–1275. doi: 10.1016/s0140-6736(74)90021-x. [DOI] [PubMed] [Google Scholar]
  16. Neter E. Endotoxins and the immune response. Curr Top Microbiol Immunol. 1969;47:82–124. doi: 10.1007/978-3-642-46160-6_5. [DOI] [PubMed] [Google Scholar]
  17. Schur P. H., Monroe M. Antibodies to ribonucleic acid in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1108–1112. doi: 10.1073/pnas.63.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schuurs A. H., Van Weemen B. K. Enzyme-immunoassay. Clin Chim Acta. 1977 Nov 15;81(1):1–40. doi: 10.1016/0009-8981(77)90410-7. [DOI] [PubMed] [Google Scholar]
  19. Winblad S., Niléhn B., Sternby N. H. Yersinia enterocolitica (Pasteurella x) in human enteric infections. Br Med J. 1966 Dec 3;2(5526):1363–1366. doi: 10.1136/bmj.2.5526.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yin E. T., Galanos C., Kinsky S., Bradshaw R. A., Wessler S., Lüderitz O., Sarmiento M. E. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria. Biochim Biophys Acta. 1972 Jan 28;261(1):284–289. doi: 10.1016/0304-4165(72)90340-6. [DOI] [PubMed] [Google Scholar]

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