Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Sep;10(3):290–292. doi: 10.1128/jcm.10.3.290-292.1979

Comparison of a slide coagglutination technique with the Minitek system for confirmation of Neisseria gonorrhoeae.

K D Hampton, R A Stallings, B L Wasilauskas
PMCID: PMC273154  PMID: 114531

Abstract

The Phadebact gonococcus test was performed on 314 bacterial isolates to compare the coagglutination technique with the Minitek system for the confirmation of Neisseria gonorrhoeae. Direct testing, using colonies of N. gonorrhoeae mixed with the Phadebact gonococcus test reagents, produced noninterpretable results in many cases. However, an alternative method, described by the manufacturer, of heating several colonies in distilled water for 20 min before testing alleviated most interpretation difficulties. Complete agreement was achieved with both the Phadebact and Minitek tests for all 236 isolates of N. gonorrhoeae. Of the 78 nongonococcal isolates tested with Phadebact, two isolates gave false-positive results, whereas one isolate was reported as noninterpretable. The Phadebact gonococcus test appears to be a simple and rapid alternative to confirmatory biochemical procedures for N. gonorrhoeae.

Full text

PDF
290

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnham M., Glynn A. A. Identification of clinical isolates of Neisseria gonorrhoeae by a coagglutination test. J Clin Pathol. 1978 Feb;31(2):189–193. doi: 10.1136/jcp.31.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. D'Amato R. F., Eriquez L. A., Tomfohrde K. M., Singerman E. Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles. J Clin Microbiol. 1978 Jan;7(1):77–81. doi: 10.1128/jcm.7.1.77-81.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DANIELSSON D. THE DEMONSTRATION OF N. GONORRHOEAE WITH THE AID OF FLUORESCENT ANTIBODIES. 1. IMMUNOLOGICAL STUDIES OF ANTIGONOCOCCAL SERA AND THEIR FLUORESCEIN-LABELLED GLOBULINS, WITH PARTICULAR REGARD TO SPECIFICITY. Acta Derm Venereol. 1963;43:451–464. [PubMed] [Google Scholar]
  4. DANIELSSON D. THE DEMONSTRATION OF N. GONORRHOEAE WITH THE AID OF FLUORESCENT ANTIBODIES. 4. STUDIES BY IMMUNOFLUORESCENCE AND DOUBLE DIFFUSION-IN-GEL TECHNIQUE ON THE ANTIGENIC RELATIONSHIP BETWEEN N. GONORRHOEAE AND OTHER NEISSERIA STRAINS. Acta Pathol Microbiol Scand. 1965;64:267–276. doi: 10.1111/apm.1965.64.2.267. [DOI] [PubMed] [Google Scholar]
  5. DEACON W. E., PEACOCK W. L., Jr, FREEMAN E. M., HARRIS A. Identification of Neisseria gonorrhoeae by means of fluorescent antibodies. Proc Soc Exp Biol Med. 1959 Jun;101(2):322–325. doi: 10.3181/00379727-101-24925. [DOI] [PubMed] [Google Scholar]
  6. Danielsson D., Johannisson G. Culture diagnosis of gonorrhoea. A comparison of the yield with selective and non-selective gonococcal culture media inoculated in the clinic and after transport of specimens. Acta Derm Venereol. 1973;53(1):75–80. [PubMed] [Google Scholar]
  7. Danielsson D., Kronvall G. Slide agglutination method for the serological identification of Neisseria gonorrhoeae with anti-gonococcal antibodies adsorbed to protein A-containing staphylococci. Appl Microbiol. 1974 Feb;27(2):368–374. doi: 10.1128/am.27.2.368-374.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hare M. J. Comparative assessment of microbiological methods for the diagnosis of gonorrhea in women. Br J Vener Dis. 1974 Dec;50(6):437–441. doi: 10.1136/sti.50.6.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollis D. G., Wiggins G. L., Weaver R. E. An unclassified gram-negative rod isolated from the pharynx on Thayer-Martin medium (selective agar). Appl Microbiol. 1972 Nov;24(5):772–777. doi: 10.1128/am.24.5.772-777.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KELLOGG D. S., Jr, DEACON W. E. A NEW RAPID IMMUNOFLUORESCENT STAINING TECHNIQUE FOR IDENTIFICATION OF TREPONEMA PALLIDUM AND NEISSERIA GONORRHOEAE. Proc Soc Exp Biol Med. 1964 Apr;115:963–965. doi: 10.3181/00379727-115-29090. [DOI] [PubMed] [Google Scholar]
  11. Lind I. Identification of Neisseria gonorrhoeae by means of fluorescent antibody technique. Acta Pathol Microbiol Scand. 1967;70(4):613–629. doi: 10.1111/j.1699-0463.1967.tb01330.x. [DOI] [PubMed] [Google Scholar]
  12. Menck H. Identification of Neisseria gonorrhoeae in cultures from tonsillo-pharyngeal specimens by means of a slide co-agglutination test (Phadebact Gonococcus Test). Acta Pathol Microbiol Scand B. 1976 Jun;84(3):139–144. doi: 10.1111/j.1699-0463.1976.tb01916.x. [DOI] [PubMed] [Google Scholar]
  13. Morse S. A., Bartenstein L. Adaptation of the Minitek system for the rapid identification of Neisseria gonorrhoeae. J Clin Microbiol. 1976 Jan;3(1):8–13. doi: 10.1128/jcm.3.1.8-13.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reddick A. A simple carbohydrate fermentation test for identification of the pathogenic Neisseria. J Clin Microbiol. 1975 Jul;2(1):72–73. doi: 10.1128/jcm.2.1.72-73.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reyn A. Recent developments in the laboratory diagnosis of gonococcal infections. Bull World Health Organ. 1969;40(2):245–255. [PMC free article] [PubMed] [Google Scholar]
  16. Vera H. D. A Simple Medium for Identification and Maintenance of the Gonococcus and Other Bacteria. J Bacteriol. 1948 Apr;55(4):531–536. doi: 10.1128/jb.55.4.531-536.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yong D. C., Prytula A. Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1978 Dec;8(6):643–647. doi: 10.1128/jcm.8.6.643-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES