Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Sep;10(3):339–342. doi: 10.1128/jcm.10.3.339-342.1979

Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen.

S A Harding, W M Scheld, M D McGowan, M A Sande
PMCID: PMC273165  PMID: 39946

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Streptococcus pneumoniae polysaccharide antigen in cerebrospinal fluid and serum. Sensitivity and specificity were determined for purified antigen preparations. Specificity was also evaluated in the rabbit meningitis model, and the sensitivity was compared to counterimmunoelectrophoresis, using the infected rabbits' cerebrospinal fluid and serum. The ELISA was a specific technique for detecting S. pneumoniae antigen. ELISA was 25 times more sensitive than counterimmunoelectrophoresis for purified antigen and resulted in an increased positivity of the cerebrospinal fluid and serum from infected rabbits. ELISA should prove very useful in the diagnosis of pneumococcal infections.

Full text

PDF
339

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coonrod J. D., Leach R. P. Antigenemia in fulminant pneumococcemia. Ann Intern Med. 1976 May;84(5):561–563. doi: 10.7326/0003-4819-84-5-561. [DOI] [PubMed] [Google Scholar]
  2. Dacey R. G., Sande M. A. Effect of probenecid on cerebrospinal fluid concentrations of penicillin and cephalosporin derivatives. Antimicrob Agents Chemother. 1974 Oct;6(4):437–441. doi: 10.1128/aac.6.4.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  4. Feigin R. D., Wong M., Shackelford P. G., Stechenberg B. W., Dunkle L. M., Kaplan S. Countercurrent immunoelectrophoresis of urine as well as of CSF and blood for diagnosis of bacterial meningitis. J Pediatr. 1976 Nov;89(5):773–775. doi: 10.1016/s0022-3476(76)80802-5. [DOI] [PubMed] [Google Scholar]
  5. Fossieck B., Jr, Craig R., Paterson P. Y. Counterimmunoelectrophoresis for rapid diagnosis of meningitis due to Diplococcus pneumoniae. J Infect Dis. 1973 Jan;127(1):106–109. doi: 10.1093/infdis/127.1.106. [DOI] [PubMed] [Google Scholar]
  6. Kenny G. E., Wentworth B. B., Beasley R. P., Foy H. M. Correlation of circulating capsular polysaccharide with bacteremia in pneumococcal pneumonia. Infect Immun. 1972 Oct;6(4):431–437. doi: 10.1128/iai.6.4.431-437.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Keydar I., Mesa-Tejada R., Ramanarayanan M., Ohno T., Fenoglio C., Hu R., Spiegelman S. Detection of viral proteins in mouse mammary tumors by immunoperoxidase staining of paraffin sections. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1524–1528. doi: 10.1073/pnas.75.3.1524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lampe R. M., Chottipitayasunondh T., Sunakorn P. Detection of bacterial antigen in pleural fluid by counterimmunoelectrophoresis. J Pediatr. 1976 Apr;88(4 Pt 1):557–560. doi: 10.1016/s0022-3476(76)80005-4. [DOI] [PubMed] [Google Scholar]
  9. Lund E., Rasmussen P. Omni-serum. A diagnostic Pneumococcus serum, reacting with the 82 known types of Pneumococcus. Acta Pathol Microbiol Scand. 1966;68(3):458–460. doi: 10.1111/apm.1966.68.3.458. [DOI] [PubMed] [Google Scholar]
  10. Miller J., Sande M. A., Gwaltney J. M., Jr, Hendley J. O. Diagnosis of pneumococcal pneumonia by antigen detection in sputum. J Clin Microbiol. 1978 May;7(5):459–462. doi: 10.1128/jcm.7.5.459-462.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  12. Perlino C. A., Shulman J. A. Detection of pneumococcal polysaccharide in the sputum of patients with pneumococcal pneumonia by counterimmunoelectrophoresis. J Lab Clin Med. 1976 Mar;87(3):496–502. [PubMed] [Google Scholar]
  13. Sande M. A., Sherertz R. J., Zak O., Strausbaugh L. J. Cephalosporin antibiotics in therapy of experimental Streptococcus pneumoniae and Haemophilus influenzae meningitis in rabbits. J Infect Dis. 1978 May;137 (Suppl):S161–S168. doi: 10.1093/infdis/137.supplement.s161. [DOI] [PubMed] [Google Scholar]
  14. Strausbaugh L. J., Mandaleris C. D., Sande M. A. Cefamandole and ampicillin therapy in experimental Haemophilus influenzae meningitis. J Infect Dis. 1977 Feb;135(2):210–216. doi: 10.1093/infdis/135.2.210. [DOI] [PubMed] [Google Scholar]
  15. Tugwell P., Greenwood B. M. Pneumococcal antigen in lobar pneumonia. J Clin Pathol. 1975 Feb;28(2):118–123. doi: 10.1136/jcp.28.2.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wheat L. J., Kohler R. B., White A. Solid-phase radioimmunoassay for detection of staphylococcal antigen in serum of rabbits with endocraditis due to Staphylococcus aureus. J Infect Dis. 1978 Aug;138(2):174–180. doi: 10.1093/infdis/138.2.174. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES