Abstract
Special solid-phase fluoroimmunoasssay protocols were used to measure the amount of immunoglobulin G (IgG) and albumin in 1,511 samples of cerebrospinal fluid. The fluoroimmunoassay is an inhibition test conducted on the surface of a plastic probe, whose fluorescence is inversely related to the concentration of spinal fluid IgG or albumin. A microcomputer interfaced with the fluorometer calculates the sample IgG or albumin from a calibration curve based on standard cerebrospinal fluid values. The test and interpretation take less than 90 min. Correlation coefficients for over 100 cerebrospinal fluid samples tested by both fluoroimmunoassay and radial immunodiffusion were: IgG, 0.90 (slope, 1.04); albumin, 0.95 (slope, 0.99). The within-run precision (coefficient of variation) was: IgG, 4.4%; albumin, 6.3%. Run-to-run precision on a midrange sample was: IgG, 7.7%, albumin, 11.7%. These findings establish the simplicity, speed, and precision of the modified fluoroimmunoassay system for specific cerebrospinal fluid proteins.
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Selected References
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- Abramowicz M. Normal values for cerebrospinal fluid protein concentration in children. What is the upper limit of normal? Clin Pediatr (Phila) 1969 May;8(5):300–301. doi: 10.1177/000992286900800518. [DOI] [PubMed] [Google Scholar]
- Berner J. J., Ciemins V. A., Schroeder E. F., Jr Radial immunodiffusion of spinal fluid: diagnostic value in multiple sclerosis. Am J Clin Pathol. 1972 Aug;58(2):145–152. doi: 10.1093/ajcp/58.2.145. [DOI] [PubMed] [Google Scholar]
- Blanchard G. C., Gardner R. Two immunofluorescent methods compared with a radial immunodiffusion method for measurement of serum immunoglobulins. Clin Chem. 1978 May;24(5):808–814. [PubMed] [Google Scholar]
- Deaton C. D., Maxwell K. W., Smith R. S., Creveling R. L. Use of laser nephelometry in the measurement of serum proteins. Clin Chem. 1976 Sep;22(9):1465–1471. [PubMed] [Google Scholar]
- Laffin R. J. A simple immunologic method for the quantitation of immunoglobulin G in human cerebrospinal fluid. J Lab Clin Med. 1970 Nov;76(5):816–823. [PubMed] [Google Scholar]
- Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
- Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
- Riddoch D., Thompson R. A. Immunoglobulin levels in the cerebrospinal fluid. Br Med J. 1970 Feb 14;1(5693):396–399. doi: 10.1136/bmj.1.5693.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ritchie R. F., Alper C. A., Graves J., Pearson N., Larson C. Automated quantitation of proteins in serum and other biologic fluids. Am J Clin Pathol. 1973 Feb;59(2):151–159. doi: 10.1093/ajcp/59.2.151. [DOI] [PubMed] [Google Scholar]
- Savory J., Heintges M. G., Killingsworth L. M., Potter J. M. Manual and automated determination of immunoglobulins in unconcentrated cerebrospinal fluid. Clin Chem. 1972 Jan;18(1):37–42. [PubMed] [Google Scholar]
- Sternberg J. C. A rate nephelometer for measuring specific proteins by immunoprecipitin reactions. Clin Chem. 1977 Aug;23(8):1456–1464. [PubMed] [Google Scholar]
- Tourtellotte W. W., Tavolato B., Parker J. A., Comiso P. Cerebrospinal fluid electroimmunodiffusion. An easy, rapid, sensitive, reliable, and valid method for the simultaneous determination of immunoglobulin-G and albumin. Arch Neurol. 1971 Oct;25(4):345–350. doi: 10.1001/archneur.1971.00490040071008. [DOI] [PubMed] [Google Scholar]