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. 1979 Sep;10(3):351–356. doi: 10.1128/jcm.10.3.351-356.1979

Reticulate bodies as single antigen in Chlamydia trachomatis serology with microimmunofluorescence.

E C Yong, J S Chinn, H D Caldwell, C C Kuo
PMCID: PMC273168  PMID: 385620

Abstract

Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibodies in human sera by microimmunofluorescence. RB reacted with all known C. trachomatis immunotypes with the same sensitivity as the homotypic EB. In routine serology with sera and endocervical secretions, the RB antigen had a sensitivity similar to that of the EB in detecting serum antibodies, endocervical secretion antibodies, and antibodies of immunoglobulin M and G classes. No false-positive reactions were detected with control sera. All positive reactions showed type-specific antibodies against an EB immunotype. RB seemed to demonstrate chlamydial group reactivity; sera from 10 psittacosis patients diagnosed clinically and serologically by complement fixation showed five positive, three equivocal, and two negative reactions. By immunofluorescence, RB appeared as distinct rings demonstrating uniform peripheral surface fluorescence at their rims. The EB appeared as pinpoint-sized dots. C/TW-3/OT RB used as a single test anitgen should provide a simple and sensitive serological assay for the detection of C. trachomatis antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caldwell H. D., Kuo C. C., Kenny G. E. Antigenic analysis of Chlamydiae by two-dimensional immunoelectrophoresis. I. Antigenic heterogeneity between C. trachomatis and C. psittaci. J Immunol. 1975 Oct;115(4):963–968. [PubMed] [Google Scholar]
  2. Caldwell H. D., Kuo C. C. Serologic diagnosis of Lymphogranuloma venereum by counterimmunoelectrophoresis with a Chlamydia trachomatis protein antigen. J Immunol. 1977 Feb;118(2):442–445. [PubMed] [Google Scholar]
  3. Holmes K. K., Handsfield H. H., Wang S. P., Wentworth B. B., Turck M., Anderson J. B., Alexander E. R. Etiology of nongonococcal urethritis. N Engl J Med. 1975 Jun 5;292(23):1199–1205. doi: 10.1056/NEJM197506052922301. [DOI] [PubMed] [Google Scholar]
  4. Kuo C. C., Wang S. P., Grayston J. T., Alexander E. R. TRIC type K, a new immunologic type of Chlamydia trachomatis. J Immunol. 1974 Aug;113(2):591–596. [PubMed] [Google Scholar]
  5. Lewis V. J., Thacker W. L., Mitchell S. H. Enzyme-linked immunosorbent assay for chlamydial antibodies. J Clin Microbiol. 1977 Nov;6(5):507–510. doi: 10.1128/jcm.6.5.507-510.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. REEVE P., TAVERNE J. Some properties of the complement-fixing antigens of the agents of trachoma and inclusion blennorrhoea and the relationship of the antigens to the developmental cycle. J Gen Microbiol. 1962 Mar;27:501–508. doi: 10.1099/00221287-27-3-501. [DOI] [PubMed] [Google Scholar]
  7. Richmond S. J., Caul E. O. Fluorescent antibody studies in chlamydial infections. J Clin Microbiol. 1975 Apr;1(4):345–352. doi: 10.1128/jcm.1.4.345-352.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Saikku P., Paavonen J. Single-antigen immunofluorescence test for chlamydial antibodies. J Clin Microbiol. 1978 Aug;8(2):119–122. doi: 10.1128/jcm.8.2.119-122.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schachter J. Chlamydial infections (third of three parts). N Engl J Med. 1978 Mar 9;298(10):540–549. doi: 10.1056/NEJM197803092981005. [DOI] [PubMed] [Google Scholar]
  10. Thomas B. J., Reeve P., Oriel J. D. Simplified serological test for antibodies to Chlamydia trachomatis. J Clin Microbiol. 1976 Jul;4(1):6–10. doi: 10.1128/jcm.4.1.6-10.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wang S. P., Grayston J. T., Alexander E. R., Holmes K. K. Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody. J Clin Microbiol. 1975 Mar;1(3):250–255. doi: 10.1128/jcm.1.3.250-255.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wang S. P., Grayston J. T., Gale J. L. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J Immunol. 1973 Mar;110(3):873–879. [PubMed] [Google Scholar]
  13. Wang S. P., Grayston J. T. Human serology in Chlamydia trachomatis infection with microimmunofluorescence. J Infect Dis. 1974 Oct;130(4):388–397. doi: 10.1093/infdis/130.4.388. [DOI] [PubMed] [Google Scholar]
  14. Wang S., Grayston J. T. Chlamydia trachomatis immunotype J. J Immunol. 1975 Dec;115(6):1711–1716. [PubMed] [Google Scholar]

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