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. Author manuscript; available in PMC: 2010 Aug 7.
Published in final edited form as: Cell. 2009 Aug 7;138(3):592–603. doi: 10.1016/j.cell.2009.07.011

Figure 7. MiR-200c Suppresses Tumorigenicity of Human Breast Cancer Stem Cells.

Figure 7

(A) A representative tumor in a mouse injected with human breast cancer stem cells is shown. CD44+CD24-/lowlineage- breast cancer stem cells were isolated from an early passage human breast xenograft tumor and infected by miR-200c expressing lentivirus or control lentivirus. 5 × 103 or 1 × 104 infected cells were injected into the breast of immunodeficient NOD/SCID mice. Tumor growth was monitored for five months after injection. (B) Tumor incidence of miRNA-expressing breast cancer stem cells (BCSCs). Six out of 13 control lentivirus infected BCSCs developed tumors after five months. One out of 13 miR-200c lentivirus infected BCSCs developed a tumor that did not expressed the GFP transgene. (C) GFP expression of tumors derived from control or miR-200c expressing lentivirus infected cells. Tumors were dissociated and GFP expression was analyzed by flow cytometry. The lentivirus contains a GFP minigene to mark virus infected cells.