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. 1979 Oct;10(4):415–418. doi: 10.1128/jcm.10.4.415-418.1979

Use of protein A-treated sera in unmasking herpes simplex virus type 1 (HSV-1) immunoglobulin A and identifying HSV-1 immunoglobulin G as the predominant neutralizing antibody.

J J Ratner, B A Sanford, K O Smith
PMCID: PMC273189  PMID: 231047

Abstract

Treatment of human sera with protein A reduced the amounts of immunoglobulin G (IgG), IgA, and IgM detected by radial immunodiffusion. This treatment also decreased the amount of herpes-specific IgG and IgM detected by radioimmunoassay, whereas it increased and even unmasked the amount of herpes-specific IgA detected. Comparison of protein A-treated sera with untreated sera indicated that herpes simplex virus type 1 IgG was responsible for more than 92 to 99% of the serum neutralizing activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Douglas R. G., Jr, Couch R. B. A prospective study of chronic herpes simplex virus infection and recurrent herpes labialis in humans. J Immunol. 1970 Feb;104(2):289–295. [PubMed] [Google Scholar]
  2. KILBOURNE E. D., HORSFALL F. L., Jr Primary herpes simplex virus infection of the adult, with a note on the relation of herpes simplex virus to recurrent aphthous stomatitis. AMA Arch Intern Med. 1951 Oct;88(4):495–502. doi: 10.1001/archinte.1951.03810100079007. [DOI] [PubMed] [Google Scholar]
  3. Kurtz J. B. Specific IgG and IgM antibody responses in herpes-simplex-virus infections. J Med Microbiol. 1974 Aug;7(3):333–341. doi: 10.1099/00222615-7-3-333. [DOI] [PubMed] [Google Scholar]
  4. Lind I., Mansa B. Further investigation of specific and non-specific adsorption of serum globulins to Staphylococcus aureus. Acta Pathol Microbiol Scand. 1968;73(4):637–645. doi: 10.1111/j.1699-0463.1968.tb03221.x. [DOI] [PubMed] [Google Scholar]
  5. Lopez C., O'Reilly R. J. Cell-mediated immune responses in recurrent herpesvirus infections. I. Lymphocyte proliferation assay. J Immunol. 1977 Mar;118(3):895–902. [PubMed] [Google Scholar]
  6. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  7. Patterson W. R., Smith K. O. Improvements of a radioimmunoassay for measurement of viral antibody in human sera. J Clin Microbiol. 1976 Aug;2(2):130–133. [PMC free article] [PubMed] [Google Scholar]
  8. SHIP I. I., ASHE W. K., SCHERP H. W. Recurrent "fever blister" and "canker sore". Tests for herpes simplex and other viruses with mammalian cell cultures. Arch Oral Biol. 1961 Feb;3:117–124. doi: 10.1016/0003-9969(61)90144-3. [DOI] [PubMed] [Google Scholar]
  9. Skaug K., Tjotta E. Diagnosis of recent Herpes simplex infections. A modified immunofluorescent test for the detection of specific Herpes simplex IgM antibodies after staphylococcal adsorption of IgG. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):323–328. [PubMed] [Google Scholar]
  10. Smith K. O., Gehle W. D. Magnetic transfer devices for use in solid-phase radioimmunoassays and enzyme-linked immunosorbent assays. J Infect Dis. 1977 Oct;136 (Suppl):S329–S336. doi: 10.1093/infdis/136.supplement_2.s329. [DOI] [PubMed] [Google Scholar]
  11. Smith K. O., Gehle W. D., McCracken A. W. Radioimmunoassay techniques for detecting naturally occurring viral antibody in human sera. J Immunol Methods. 1974 Oct;5(4):337–344. doi: 10.1016/0022-1759(74)90017-9. [DOI] [PubMed] [Google Scholar]
  12. Tokumaru T. A possible role of gamma-A-immunoglobulin in herpes simplex virus infection in man. J Immunol. 1966 Aug;97(2):248–259. [PubMed] [Google Scholar]

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