Abstract
The counterimmunoelectrophoresis (CIE) technique was developed as a diagnostic procedure for ram epididymitis caused by Brucella ovis. CIE test results with sera from naturally and experimentally infected male sheep compared favorably with those obtained by gel diffusion and complement fixation employing the same B. ovis surface R antigen. The main advantage of CIE over gel diffusion consists in a significant reduction of the time required to detect precipitin formation, whereas both methods obviate several of the difficulties encountered with complement fixation tests for B. ovis.
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BIBERSTEIN E. L., McGOWAN B. Epididymitis in rams; studies on laboratory diagnosis. Cornell Vet. 1958 Jan;48(1):31–44. [PubMed] [Google Scholar]
- Fedorko J., Fossieck B. E., Jr, Parker R. H. Counterimmunoelectrophoresis of staphylococcal antibody. J Clin Microbiol. 1976 Jun;3(6):599–603. doi: 10.1128/jcm.3.6.599-603.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myers D. M. Field evaluation of the gel diffusion test for the diagnosis of ram epididymitis caused by Brucella ovis. Appl Microbiol. 1973 Dec;26(6):855–857. doi: 10.1128/am.26.6.855-857.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myers D. M., Jones L. M., Varela-Diaz V. M. Studies of antigens for complement fixation and gel diffusion tests in the diagnosis of infections caused by Brucella ovis and other Brucella. Appl Microbiol. 1972 May;23(5):894–902. doi: 10.1128/am.23.5.894-902.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myers D. M., Siniuk A. A. Preliminary report on the development of a diffusion-in-gel method for the diagnosis of ram epididymitis. Appl Microbiol. 1970 Feb;19(2):335–337. doi: 10.1128/am.19.2.335-337.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myers D. M., Varela-Díaz V. M., Coltorti E. A. Comparative sensitivity of gel-diffusion and tube agglutination tests for the detection of Brucella canis antibodies in experimentally infected dogs. Appl Microbiol. 1974 Jul;28(1):1–4. doi: 10.1128/am.28.1.1-4.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]