Abstract
An enzyme-linked immunosorbent assay (ELISA) for measuring antibodies against each of 14 polysaccharides in contemporary pneumococcal vaccine is described, and the findings of tests of paired sera from vaccinated human subjects are compared with those obtained by radioimmunoassay. The findings were in very poor agreement, and this appears to be due to the lesser ability of the ELISA procedure to measure antibody of low avidity. The ELISA procedure described here is not considered to be a satisfactory substitute for radioimmunoassay for measuring antibody responses to pneumococcal vaccine.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berntsson E., Broholm K. A., Kaijser B. Serological diagnosis of pneumococcal disease with enzyme-linked immunosorbent assay (ELISA). Scand J Infect Dis. 1978;10(3):177–181. doi: 10.3109/inf.1978.10.issue-3.04. [DOI] [PubMed] [Google Scholar]
- Butler J. E., Feldbush T. L., McGivern P. L., Stewart N. The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity. Immunochemistry. 1978 Feb;15(2):131–136. doi: 10.1016/0161-5890(78)90053-6. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
- FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Weibel R. E., Vella P. P., McLean A. A., Woodhour A. F., Davidson W. L., Hilleman M. R. Studies in human subjects of polyvalent pneumococcal vaccines (39894). Proc Soc Exp Biol Med. 1977 Oct;156(1):144–150. doi: 10.3181/00379727-156-39894. [DOI] [PubMed] [Google Scholar]