Abstract
The morphological and biochemical changes that occur after inoculation of sterile blood into a blood culture medium (tryptic soy broth) with sodium polyanetholesulfonate and CO2 were investigated. Cellular changes, pH, PCO2, and PO2 were monitored and evaluated. Erythrocytes became crenated and developed precipitated hemoglobin inclusions within 4 h. The lymphocytes appeared morphologically intact at 24 h, and by 48 h a few cells had undergone transformation. Many neutrophils were vacuolated at 24 h. Neutrophils capable of phagocytizing Staphylococcus aureus were observed after 18 h of incubation. Identifiable eosinophiles were present on day 6 of the study. A decrease in PO2 in the unvented bottles from 44.4 to 8 mm of Hg occurred by 24 h. PO2 remained low for 6 days, after which a slight increase occurred. An increase in PO2 in the vented bottle from 51 to 58 mm of Hg occurred by 24 h of incubation. In both the vented and unvented bottles the PCO2 increased. This increase was markedly more rapid in the unvented bottle. From a pH of 7.06 a decrease occurred for the first 24 h after inoculation, with the pH stabilizing at 6.8 in the vented bottles and at 6.6 in the unvented bottles. The biochemical changes that occurred in the vented culture bottles stabilized more rapidly than those of the unvented bottles. Changes caused by the addition of sterile blood to a blood culture medium resulted in conditions which departed considerably from accepted optima for the isolation of clinically important microorganisms. The phagocytosis of organisms that occurred may also have reduced the yield.
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- Dorn G. L., Burson G. G., Haynes J. R. Blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol. 1976 Mar;3(3):258–263. doi: 10.1128/jcm.3.3.258-263.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dorn G. L., Haynes J. R., Burson G. G. Blood culture technique based on centrifugation: developmental phase. J Clin Microbiol. 1976 Mar;3(3):251–257. doi: 10.1128/jcm.3.3.251-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall M., Warren E., Washington J. A., 2nd Detection of bacteremia with liquid media containing sodium polyanetholsulfonate. Appl Microbiol. 1974 Jan;27(1):187–191. doi: 10.1128/am.27.1.187-191.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kagan R. L., Schuette W. H., Zierdt C. H., MacLowry J. D. Rapid automated disgnosis of bacteremia by impedance detection. J Clin Microbiol. 1977 Jan;5(1):51–57. doi: 10.1128/jcm.5.1.51-57.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts G. D., Horstmeier C., Hall M., Washington J. A., 2nd Recovery of yeast from vented blood culture bottles. J Clin Microbiol. 1975 Jul;2(1):18–20. doi: 10.1128/jcm.2.1.18-20.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts G. D., Washington J. A., 2nd Detection of fungi in blood cultures. J Clin Microbiol. 1975 Mar;1(3):309–310. doi: 10.1128/jcm.1.3.309-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosner R. Effect of various anticoagulants and no anticoagulant on ability to isolate bacteria directly from parallel clinical blood specimens. Am J Clin Pathol. 1968 Feb;49(2):216–219. doi: 10.1093/ajcp/49.2.216. [DOI] [PubMed] [Google Scholar]
- Rosner R. Evaluation of four blood culture systems using parallel culture methods. Appl Microbiol. 1974 Aug;28(2):245–247. doi: 10.1128/am.28.2.245-247.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smolka L. R., Nelson F. E., Kelley L. M. Interaction of pH and NaCl on enumeration of heat-stressed Staphylococcus aureus. Appl Microbiol. 1974 Mar;27(3):443–447. doi: 10.1128/am.27.3.443-447.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sullivan N. M., Sutter V. L., Finegold S. M. Practical aerobic membrane filtration blood culture technique: clinical blood culture trial. J Clin Microbiol. 1975 Jan;1(1):37–43. doi: 10.1128/jcm.1.1.37-43.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]