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. 1979 Oct;10(4):563–566. doi: 10.1128/jcm.10.4.563-566.1979

Adhesion to a human cell line by Escherichia coli strains isolated during urinary tract infections.

G Chabanon, C L Hartley, M H Richmond
PMCID: PMC273215  PMID: 393720

Abstract

It has been shown that some, but not all, Escherichia coli strains isolated from urine adhere, in vitro, to the surface of uroepithelial or vaginal cells. In the present study, 212 strains, isolated from urine of 212 infected patients, were tested for adhesion by using an in vitro human cell line assay. A variable degree of attachment to the cell monolayer was detected in these strains. From patients with cystitis, only 19 (9.7%) of the 195 strains examined were adherent, whereas 5 (29.4%) of the 17 pyelonephritis strains had similar properties (P less than 0.05). To investigate the incidence of adhesion in the clinical manifestations of urinary tract infection, a sample of patients was picked at random from those with cystitis. During cystitis caused by adhesive bacteria, patients suffer more often from macroscopic hematuria than from dysuria, frequency, or recurrency (P less than 0.05). This study shows that E. coli strains isolated from urine samples possess a strikingly difference in capacity to adhere to a human cell line surface as demonstrated previously with uroepithelial or vaginal cells. Moreover, according to these data, the adhesion of E. coli may be considered as a virulent factor and would play a part in the infection of the urinary tract in humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eden C. S., Eriksson B., Hanson L. A. Adhesion of Escherichia coli to human uroepithelial cells in vitro. Infect Immun. 1977 Dec;18(3):767–774. doi: 10.1128/iai.18.3.767-774.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edén C. S., Hanson L. A., Jodal U., Lindberg U., Akerlund A. S. Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. Lancet. 1976 Sep 4;1(7984):490–492. [PubMed] [Google Scholar]
  3. Fairley K. F., Carson N. E., Gutch R. C., Leighton P., Grounds A. D., Laird E. C., McCallum P. H., Sleeman R. L., O'Keefe C. M. Site of infection in acute urinary-tract infection in general practice. Lancet. 1971 Sep 18;2(7725):615–618. doi: 10.1016/s0140-6736(71)80066-1. [DOI] [PubMed] [Google Scholar]
  4. Fowler J. E., Jr, Stamey T. A. Studies of introital colonization in women with recurrent urinary infections. VII. The role of bacterial adherence. J Urol. 1977 Apr;117(4):472–476. doi: 10.1016/s0022-5347(17)58501-8. [DOI] [PubMed] [Google Scholar]
  5. Gibbons R. J., Houte J. V. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. doi: 10.1146/annurev.mi.29.100175.000315. [DOI] [PubMed] [Google Scholar]
  6. Glynn A. A., Brumfitt W., Howard C. J. K antigens of Escherichia coli and renal involvement in urinary-tract infections. Lancet. 1971 Mar 13;1(7698):514–516. [PubMed] [Google Scholar]
  7. Hanson L. A., Ahlstedt S., Fasth A., Jodal U., Kaijser B., Larsson P., Lindberg U., Olling S., Sohl-Akerlund A., Svanborg-Edén C. Antigens of Escherichia coli, human immune response, and the pathogenesis of urinary tract infections. J Infect Dis. 1977 Aug;136 (Suppl):S144–S149. doi: 10.1093/infdis/136.supplement.s144. [DOI] [PubMed] [Google Scholar]
  8. Hartley C. L., Robbins C. M., Richmond M. H. Quantitative assessment of bacterial adhesion to eukaryotic cells of human origin. J Appl Bacteriol. 1978 Aug;45(1):91–97. doi: 10.1111/j.1365-2672.1978.tb04202.x. [DOI] [PubMed] [Google Scholar]
  9. Mårdh P. A., Westtöm L. Adherence of bacterial to vaginal epithelial cells. Infect Immun. 1976 Mar;13(3):661–666. doi: 10.1128/iai.13.3.661-666.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ofek I., Mirelman D., Sharon N. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature. 1977 Feb 17;265(5595):623–625. doi: 10.1038/265623a0. [DOI] [PubMed] [Google Scholar]
  11. STAMEY T. A., GOVAN D. E., PALMER J. M. THE LOCALIZATION AND TREATMENT OF URINARY TRACT INFECTIONS: THE ROLE OF BACTERICIDAL URINE LEVELS AS OPPOSED TO SERUM LEVELS. Medicine (Baltimore) 1965 Jan;44:1–36. doi: 10.1097/00005792-196501000-00001. [DOI] [PubMed] [Google Scholar]
  12. Sanford J. P. Urinary tract symptoms and infections. Annu Rev Med. 1975;26:485–498. doi: 10.1146/annurev.me.26.020175.002413. [DOI] [PubMed] [Google Scholar]
  13. Smith H. Microbial surfaces in relation to pathogenicity. Bacteriol Rev. 1977 Jun;41(2):475–500. doi: 10.1128/br.41.2.475-500.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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