Abstract
Adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. Indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these strains have a unique single-cell fluorescence pattern. Hematoxylin and eosin staining reveals adenovirus-like inclusion bodies in the same distribution as fluorescent cells. Pretreatment of nongrowing adenovirus with convalescent-phase patient serum neutralized its ability to infect the cell culture and produce fluorescent cells. Indirect immunofluorescence microscopy compared favorably with electron microscopy in demonstrating virus in the stools of patients.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Banatvala J. E., Totterdell B., Chrystie I. L., Woode G. N. In-vitro detection of human rotaviruses. Lancet. 1975 Oct 25;2(7939):821–821. doi: 10.1016/s0140-6736(75)80057-2. [DOI] [PubMed] [Google Scholar]
- DUNCAN I. B., HUTCHISON J. G. Type-3 adenovirus infection with gastrointestinal symptoms. Lancet. 1961 Mar 11;1(7176):530–532. doi: 10.1016/s0140-6736(61)91599-9. [DOI] [PubMed] [Google Scholar]
- Fulton R. E., Middleton P. J. Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children. Infect Immun. 1974 Jul;10(1):92–101. doi: 10.1128/iai.10.1.92-101.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JONCAS J., PAVILANIS V. Diarrhoea and vomiting in infancy and childhood: viral studies. Can Med Assoc J. 1960 May 28;82:1108–1113. [PMC free article] [PubMed] [Google Scholar]
- Johansson M. E., Wadell G., Jacobsson P. A., Svensson L. Preparation of specific antisera against adenoviruses by affinity bead immunization (ABI). J Immunol Methods. 1979;26(2):141–149. doi: 10.1016/0022-1759(79)90077-2. [DOI] [PubMed] [Google Scholar]
- Madeley C. R. Viruses in the stools. J Clin Pathol. 1979 Jan;32(1):1–10. doi: 10.1136/jcp.32.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mankikar S. D., Middleton P. J., Petric M. Counterimmunoelectrophoresis assay for detection of adenovirus antigen. J Clin Microbiol. 1979 Aug;10(2):253–255. doi: 10.1128/jcm.10.2.253-255.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Am J Dis Child. 1977 Jul;131(7):733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
- Morris C. A., Flewett T. H., Bryden A. S., Davies H. Epidemic viral enteritis in a long-stay children's ward. Lancet. 1975 Jan 4;1(7897):4–5. doi: 10.1016/s0140-6736(75)92370-3. [DOI] [PubMed] [Google Scholar]
- ROSS J. G., POTTER C. W., ZACHARY R. B. Adenovirus infection in association with intussusception in infancy. Lancet. 1962 Aug 4;2(7249):221–223. doi: 10.1016/s0140-6736(62)92315-2. [DOI] [PubMed] [Google Scholar]
- Reich P. R., Baum S. G., Rose J. A., Rowe W. P., Weissman S. M. Nucleic acid homology studies of adenovirus type 7-SV40 interactions. Proc Natl Acad Sci U S A. 1966 Feb;55(2):336–341. doi: 10.1073/pnas.55.2.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHMIDT N. J., LENNETTE E. H., HO H. H. COMPARATIVE SENSITIVITY OF HUMAN FETAL DIPLOID KIDNEY CELL STRAINS AND MONKEY KIDNEY CELL CULTURES FOR ISOLATION OF CERTAIN HUMAN VIRUSES. Am J Clin Pathol. 1965 Apr;43:297–301. doi: 10.1093/ajcp/43.4.297. [DOI] [PubMed] [Google Scholar]
- Whitelaw A., Davies H., Parry J. Eelectron microscopy of fatal adenovirus gastroenteritis. Lancet. 1977 Feb 12;1(8007):361–361. doi: 10.1016/s0140-6736(77)91158-8. [DOI] [PubMed] [Google Scholar]
- Woode G. N., Bridger J. C., Hall G., Dennis M. J. The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Res Vet Sci. 1974 Jan;16(1):102–105. [PubMed] [Google Scholar]