Figure 4.
Expression of 3βHSD, P450c21, and reduced nicotinamide adenine dinucleotide phosphate-CPR in rhesus adrenal microsomes of neonatal rhesus macaques from birth to 3 months of age. A, Densitometric analysis of Western immunoblots of 3βHSD expression in perinatal and neonatal rhesus adrenal microsomes. Band intensities were expressed a percentage of a standard (20 μg from a pool of marmoset adrenal microsomes) included in each gel. Levels of 3βHSD increased with age (R2 = 0.32; P < 0.05). B, Representative Western immunoblot of 3βHSD used for densitometry to detect age-related changes in rhesus neonatal adrenal microsomes (20 μg/lane). C, Representative Western immunoblot of P450c21 used for densitometry. No age-related changes in P450c21 expression were found in rhesus neonatal adrenal microsomes (20 μg/lane). D, Representative Western immunoblot of CPR used for densitometry. No age-related changes in CPR expression were found in rhesus neonatal adrenal microsomes (20 μg/lane).