Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Nov;10(5):650–656. doi: 10.1128/jcm.10.5.650-656.1979

Destruction of bacterial viruses in serum by heat and radiation under conditions that sustain the ability of serum to support growth of cells in suspended culture.

R L Ward
PMCID: PMC273240  PMID: 544633

Abstract

Methods for inactivating bacterial viruses in serum were developed through the use of heat and ionizing radiation, and the effects of these treatments on the growth rates of cultured cells were tested. Viruses chosen for this study were the radiation-resistant bacteriphage f2 and heat-resistant phage T4. The viabilities of these phages were reduced more than 2 and 4 orders of magnitude, respectively, by a treatment at 60 degrees C for 30 min followed by 420 krads of ionizing radiation. Simultaneous application of heat and radiation caused a considerably greater reduction in viability of both phages in serum, but also caused a significant decrease in the growth rates of L cells in medium supplemented with serum treated in this manner. Treatment of serum with these same doses but given in the sequential fashion of heat followed by radiation caused little or no change in the growth rates of L cells. Finally, it was found that simultaneous treatment of serum with these doses of heat and radiation had little effect on the growth rates of either HeLa or Chinese hamster cells.

Full text

PDF
650

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS W. R., POLLARD E. Combined thermal and primary ionization effects on a bacterial virus. Arch Biochem Biophys. 1952 Apr;36(2):311–322. doi: 10.1016/0003-9861(52)90416-5. [DOI] [PubMed] [Google Scholar]
  2. Bahnemann H. G. Inactivation of viruses in serum with binary ethyleneimine. J Clin Microbiol. 1976 Feb;3(2):209–210. doi: 10.1128/jcm.3.2.209-210.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benz E. W., Jr, Moses H. L. Small, virus-like particles detected in bovine sera by electron microscopy. J Natl Cancer Inst. 1974 Jun;52(6):1931–1934. doi: 10.1093/jnci/52.6.1931. [DOI] [PubMed] [Google Scholar]
  4. Calafat J., Hageman P. C., Ressang A. A. Virus-like particles in bovine sera for tissue culture. J Gen Virol. 1976 Oct;33(1):151–154. doi: 10.1099/0022-1317-33-1-151. [DOI] [PubMed] [Google Scholar]
  5. Chu F. C., Johnson J. B., Orr H. C., Probst P. G., Petricciani J. C. Bacterial virus contamination of fetal bovine sera. In Vitro. 1973 Jul-Aug;9(1):31–34. doi: 10.1007/BF02615986. [DOI] [PubMed] [Google Scholar]
  6. DiGioia G. A., Licciardello J. J., Nickerson J. T., Goldblith S. A. Effect of temperature on radiosensitivity of Newcastle disease virus. Appl Microbiol. 1970 Mar;19(3):455–457. doi: 10.1128/am.19.3.455-457.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dugan V. L., Trujillo R. Heat-accelerated radioinactivation of attenuated poliovirus. Radiat Environ Biophys. 1975 Oct 2;12(3):187–195. doi: 10.1007/BF01327346. [DOI] [PubMed] [Google Scholar]
  8. Fong C. K., Gross P. A., Hsiung G. D., Swack N. S. Use of electron microscopy for detection of viral and other microbial contaminants in bovine sera. J Clin Microbiol. 1975 Feb;1(2):219–224. doi: 10.1128/jcm.1.2.219-224.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GINOZA W. RADIOSENSITIVE MOLECULAR WEIGHT OF SINGLE-STRANDED VIRUS NUCLEIC ACIDS. Nature. 1963 Aug 3;199:453–456. doi: 10.1038/199453a0. [DOI] [PubMed] [Google Scholar]
  10. Geier M. R., Attallah A. F., Merril C. R. Characterization of Escherichia coli bacterial viruses in commercial sera,. In Vitro. 1975 Jan-Feb;11(1):55–58. doi: 10.1007/BF02615323. [DOI] [PubMed] [Google Scholar]
  11. Geier M. R., Merril C. R. Lambda phage transcription in human fibroblasts. Virology. 1972 Mar;47(3):638–643. doi: 10.1016/0042-6822(72)90553-3. [DOI] [PubMed] [Google Scholar]
  12. Kniazeff A. J. Viruses infecting cattle and their role as endogenous contaminants of cell cultures. Natl Cancer Inst Monogr. 1968 Dec;29:123–132. [PubMed] [Google Scholar]
  13. Kniazeff A. J., Wopschall L. J., Hopps H. E., Morris C. S. Detection of bovine viruses in fetal bovine serum used in cell culture. In Vitro. 1975 Nov-Dec;11(6):400–403. doi: 10.1007/BF02616377. [DOI] [PubMed] [Google Scholar]
  14. Kolata G. B. Phage in live virus vaccines: are they harmful to people? Science. 1975 Feb 14;187(4176):522–523. doi: 10.1126/science.187.4176.522. [DOI] [PubMed] [Google Scholar]
  15. MANKIEWICZ E. BACTERIOPHAGES THAT LYSE MYCOBACTERIA AND CORYNEBACTERIA, AND SHOW CYTOPATHOGENIC EFFECT ON TISSUE CULTURES OF RENAL CELLS OF CERCOPITHECUS AETHIOPS: A PRELIMINARY COMMUNICATION. Can Med Assoc J. 1965 Jan 2;92:31–33. [PMC free article] [PubMed] [Google Scholar]
  16. Merril C. R., Friedman T. B., Attallah A. F., Geier M. R., Krell K., Yarkin R. Isolation of bacteriophages from commercial sera. In Vitro. 1972 Sep-Oct;8(2):91–93. doi: 10.1007/BF02615965. [DOI] [PubMed] [Google Scholar]
  17. Merril C. R., Geier M. R., Petricciani J. C. Bacterial virus gene expression in human cells. Nature. 1971 Oct 8;233(5319):398–400. doi: 10.1038/233398a0. [DOI] [PubMed] [Google Scholar]
  18. Michalski F. J., Hsiung G. D. Persistent infection with bovine herpesvirus-1 (infectious bovine rhinotracheitis virus) in cultured hamster cells. In Vitro. 1976 Oct;12(10):682–686. doi: 10.1007/BF02797471. [DOI] [PubMed] [Google Scholar]
  19. Michalski F., Hsiung G. D. Malignant transformation of hamster cells following infection with bovine herpesvirus (infectious bovine rhinotracheitis virus. Proc Soc Exp Biol Med. 1975 Mar;148(3):891–896. doi: 10.3181/00379727-148-38655. [DOI] [PubMed] [Google Scholar]
  20. Molander C. W., Kniazeff A. J., Boone C. W., Paley A., Imagawa D. T. Isolation and characterization of viruses from fetal calf serum. In Vitro. 1971 Nov-Dec;7(3):168–173. doi: 10.1007/BF02617962. [DOI] [PubMed] [Google Scholar]
  21. Moody E. E., Trousdale M. D., Jorgensen J. H., Shelokov A. Bacteriophages and endotoxin in licensed live-virus vaccines. J Infect Dis. 1975 May;131(5):588–591. doi: 10.1093/infdis/131.5.588. [DOI] [PubMed] [Google Scholar]
  22. Orr H. C., Probst P. G., Littlejohn D. C., Chu F. C., Petricciani J. C. Persistence of bacteriophages in experimentally infected cell cultures. Appl Microbiol. 1974 Jun;27(6):1105–1107. doi: 10.1128/am.27.6.1105-1107.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Orr H. C., Sibinovic K. H., Probst P. G., Hochstein H. D., Littlejohn D. C. Bacteriological activity in unfiltered calf sera collected for tissue culture use. In Vitro. 1975 Jul-Aug;11(4):230–233. doi: 10.1007/BF02616338. [DOI] [PubMed] [Google Scholar]
  24. Orr H. C., Weetall H. H., Probst P. G., Littlejohn D. C., Chu F. C., Johnson J. B., Petricciani J. C. Elimination of bacteriophages from tissue culture serum by affinity chromatography. J Clin Microbiol. 1976 Apr;3(4):402–405. doi: 10.1128/jcm.3.4.402-405.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Petricciani J. C., Chu F. C., Johnson J. B., Meyer H. M., Jr Bacteriophages in live virus vaccines. Proc Soc Exp Biol Med. 1973 Dec;144(3):789–792. doi: 10.3181/00379727-144-37683. [DOI] [PubMed] [Google Scholar]
  26. Petricciani J. C., Hsu T. C., Stock A. D., Turner J. H., Wenger S. L., Elisberg B. L. Bacteriophages, vaccines, and people: an assessment of risk. Proc Soc Exp Biol Med. 1978 Jul;158(3):378–382. doi: 10.3181/00379727-158-40208. [DOI] [PubMed] [Google Scholar]
  27. Pollard E. C., Solosko W. The thermal inactivation of T4 and lambda bacteriophage. Biophys J. 1971 Jan;11(1):66–74. doi: 10.1016/S0006-3495(71)86195-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Trujillo R., Dugan V. L. Synergistic inactivation of viruses by heat and ionizing radiation. Biophys J. 1972 Jan;12(1):92–113. doi: 10.1016/S0006-3495(72)86073-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Van der Maaten M. J., Hubbert W. T., Boothe A. D., Bryner J. H., Estes P. C. Isolations of bovine syncytial virus from maternal and fetal blood. Am J Vet Res. 1973 Mar;34(3):341–343. [PubMed] [Google Scholar]
  30. Wenger S. L., Turner J. H., Petricciani J. C. The cytogenetic, proliferative and viability effects of four bacteriophages on human lymphocytes. In Vitro. 1978 Jun;14(6):543–549. doi: 10.1007/BF02616097. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES