Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Nov;10(5):673–676. doi: 10.1128/jcm.10.5.673-676.1979

Evaluation of a biphasic medium for blood cultures.

M M Hall, C A Mueske, D M Ilstrup, J A Washington 2nd
PMCID: PMC273245  PMID: 397222

Abstract

A study comparing the recovery of microorganisms from a transiently vented biphasic brain heart infusion medium bottle and a vacuum bottle containing tryptic soy broth demonstrated that growth was initially detected on the slant of the biphasic bottle or on a routine subculture of either broth in nearly 50% of instances. All organisms were isolated equally well in both bottles, with the exception of Staphylococcus aureus, which was isolated significantly more frequently from the biphasic bottle, and anaerobic bacteria, which were isolated significantly more frequently from the tryptic soy broth bottle.

Full text

PDF
673

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hall M. M., Ilstrup D. M., Washington J. A., 2nd Comparison of three blood culture media with tryptic soy broth. J Clin Microbiol. 1978 Sep;8(3):299–301. doi: 10.1128/jcm.8.3.299-301.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harkness J. L., Hall M., Ilstrup D. M., Washington J. A., 2nd Effects of atmosphere of incubation and of routine subcultures on detection of bacteremia in vacuum blood culture bottles. J Clin Microbiol. 1975 Oct;2(4):296–299. doi: 10.1128/jcm.2.4.296-299.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Murray P. R., Sondag J. E. Evaluation of routine subcultures of macroscopically negative blood cultures for detection of anaerobes. J Clin Microbiol. 1978 Oct;8(4):427–430. doi: 10.1128/jcm.8.4.427-430.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Paisley J. W., Rosenblatt J. E., Hall M., Washington J. A., 2nd Evaluation of a routine anaerobic subculture of blood cultures for detection of anaerobic bacteremia. J Clin Microbiol. 1978 Dec;8(6):764–766. doi: 10.1128/jcm.8.6.764-766.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Roberts G. D., Washington J. A., 2nd Detection of fungi in blood cultures. J Clin Microbiol. 1975 Mar;1(3):309–310. doi: 10.1128/jcm.1.3.309-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. SCOTT E. G. A practical blood culture procedure. Am J Clin Pathol. 1951 Mar;21(3):290–294. doi: 10.1093/ajcp/21.3_ts.290. [DOI] [PubMed] [Google Scholar]
  7. Todd J. K., Roe M. H. Rapid detection of bacteremia by an early subculture technic. Am J Clin Pathol. 1975 Nov;64(5):694–699. doi: 10.1093/ajcp/64.5.694. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES