Abstract
Campylobacteriosis is a collective description for infectious diseases caused by members of the bacterial genus Campylobacter. The only form of campylobacteriosis of major public health importance is Campylobacter enteritis due to C. jejuni and C. coli. Research and control efforts on the disease have been conducted more often in developed countries than developing countries. However, because of the increasing incidence, expanding spectrum of infections, potential of HIV-related deaths due to Campylobacter, and the availability of the complete genome sequence of C. jejuni NCTC 11168, interest in campylobacteriosis research and control in developing countries is growing. We present the distinguishing epidemiologic and clinical features of Campylobacter enteritis in developing countries relative to developed countries. National surveillance programs and international collaborations are needed to address the substantial gaps in the knowledge about the epidemiology of campylobacteriosis in developing countries.
Keywords: Campylobacter, Campylobacter infections, developing countries, developed countries, epidemiology, public health
Campylobacteriosis is a collective description for infectious diseases caused by members of the bacterial genus Campylobacter. The only form of campylobacteriosis of major public health importance is Campylobacter enteritis due to C. jejuni and C. coli (1). The rate of Campylobacter infections worldwide has been increasing, with the number of cases often exceeding those of salmonellosis and shigellosis (2). This increase, as well as the expanding spectrum of diseases caused by the organisms, necessitates a clearer understanding of the epidemiology and control of campylobacteriosis.
Surveillance and control of diseases of public health importance in developing countries have focused on diseases such as malaria, tuberculosis, trypanosomiasis, onchocerciasis, and schistosomiasis (3). Programs for diarrhea and acute respiratory illness also exist (4). These programs have extensive support from the World Health Organization (WHO).
Campylobacter is one of the most frequently isolated bacteria from stools of infants with diarrhea in developing countries—a result of contaminated food or water (5,6). However, national surveillance programs for campylobacteriosis generally do not exist in most developing countries despite the substantial burden of disease. Most data available on campylobacteriosis in developing countries were collected as a result of support provided by WHO to many laboratories in developing countries, including grants for epidemiologic studies and Lior serotyping antisera provided by the Public Health Service of Canada (5,7). The number of reviews and updates on human campylobacteriosis in developed countries (8–11) is greater than that for developing countries (5,6). This disparity may be because Campylobacter-associated diarrhea in developing countries is not pathogenic in patients >6 months of age. However, a community-based longitudinal study provided evidence that infection could be pathogenic beyond the first 6 months of life in developing countries (12). Furthermore, the sequencing and publication of the complete genome of C. jejuni NCTC 11168 have heralded a renaissance of interest in this organism, offering researchers worldwide, including in developing countries, novel ways to contribute to the understanding the organism’s biology (13). Thus, to promote research and control of campylobacteriosis in developing countries, review information on human campylobacteriosis in these countries is urgently needed. We present the distinguishing features of campylobacteriosis in developing countries relative to developed countries.
Incidence
Generally, developing countries do not have national surveillance programs for campylobacteriosis; therefore, incidence values in terms of number of cases for a population do not exist. Availability of national surveillance programs in developed countries has facilitated monitoring of sporadic cases as well as outbreaks of human campylobacteriosis (2,8–11). Most estimates of incidence in developing countries are from laboratory-based surveillance of pathogens responsible for diarrhea. Campylobacter isolation rates in developing countries range from 5 to 20% (6). Table 1 shows isolation rates for some countries according to WHO regions from studies of diarrhea in children <5 years old (14–25). Despite the lack of incidence data from national surveys, case-control community-based studies have provided estimates of 40,000 to 60,000/100,000 for children <5 years of age (6,12). In contrast, the figure for developed countries is 300/100,000 (8). Estimates in the general population in developing and developed countries are similar, approximately 90/100,000 (5,6,8), confirming the observation that campylobacteriosis is often a pediatric disease in developing countries. The isolation and incidence rates in some developing countries have increased since their initial reports (17). This increase has often been attributed to improved diagnostic methods, but an actual increase in incidence was observed in Campylobacter-associated diarrhea in the Caribbean island of Curaçao (26).
Table 1. Isolation rates of Campylobacter from diarrhea specimens from <5-year-olds in selected developing countries.
| WHO region and country | Isolation rate (%) | Reference |
|---|---|---|
| Africa | ||
| Algeria | 17.7 | 14 |
| Cameroon | 7.7 | 15 |
| Ethiopia | 13.8 | 16 |
| Nigeria | 16.5 | 17 |
| Tanzania | 18.0 | 18 |
| Zimbabwe | 9.3 | 19 |
| Americas | ||
| Brazil | 9.9 | 20 |
| Guatemala | 12.1 | 21 |
| Eastern Mediterranean | ||
| Egypt | 9.0 | 12 |
| Jordan | 5.5 | 22 |
| Southeast Asia | ||
| Bangladesh | 17.4 | 23 |
| Thailand | 13.0 | 24 |
| Western Pacific | ||
| Laos | 12.1 | 25 |
WHO = World Health Organization.
Age of Infection
In developing countries, Campylobacter is the most commonly isolated bacterial pathogen from <2-year-old children with diarrhea (Table 2). The disease does not appear to be important in adults. In contrast, infection occurs in adults and children in developed countries. Poor hygiene and sanitation and the close proximity to animals in developing countries all contribute to easy and frequent acquisition of any enteric pathogen, including Campylobacter. Although infections in infants appear to decline with age (Table 2), a comprehensive community-based cohort study in Egypt has shown that infection could be pathogenic regardless the age of the child, underscoring the need for strengthening prevention and control strategies for campylobacteriosis (12).
Table 2. Age of patients with Campylobacter infection in selected developing countries.
Polymicrobial Infections Involving Campylobacter
Campylobacter is isolated relatively frequently with another enteric pathogen in patients with diarrhea in developing countries. In some cases half or more patients with Campylobacter enteritis also had other enteric pathogens (23,30). Organisms reported include Escherichia coli, Salmonella, Shigella, Giardia lamblia, and Rotavirus. Polymicrobial infections involving Campylobacter are rare in developed countries (5,6).
Isolation of Campylobacter in Healthy Children
The recovery of Campylobacter organisms from children without diarrhea is common in developing countries. In some reports the isolation rates for symptomatic and asymptomatic children were not statistically significant. Values as high as 14.9% in controls have been observed (14). Acquisition of the pathogen because of poor sanitation and contact with animals early in life may explain the isolation from healthy children. Campylobacter is not frequently recovered from asymptomatic persons in developed countries, as observed in the Netherlands, where a 0.5% isolation rate has been reported (9).
Seasonal Variation
In developing countries, Campylobacter enteritis has no seasonal preference; in contrast, in developed countries epidemics occur in summer and autumn (2). Isolation peaks vary from one country to another and also within countries (12,31,32). The lack of seasonal preference may be due to lack of extreme temperature variation as well as lack of adequate surveillance for epidemics (5,6).
Distribution of Campylobacter Species
C. jejuni and C. coli are the two main species isolated in developing countries. The isolation rate of C. jejuni exceeds that of C. coli, similar to observations in most developed countries (8,9). Lior biotyping and serotyping methods have been used in developing countries to subtype strains of C. jejuni and C. coli (5,6). Table 3 shows the distribution of the subtypes from three African countries. Biotype I was the most common, followed by biotype II. The prevalence of specific serotypes only in symptomatic children may indicate virulence traits or treatment, in cases of gastroenteritis (33). Furthermore, correlation between biotypes and serotypes isolated from humans and animals indicates that campylobacteriosis is zoonotic (36). Penner serotyping scheme and DNA-based typing, extensively used in developed countries, have been proposed for use in developing countries (37).
Table 3. Distribution of Campylobacter jejuni and C. coli biotypes and serotypes in three African countries.
Species other than C.jejuni and C. coli—such as C. upsaliensis, C. concisus, and aerotolerant campylobacters (Arcobacter)—may also be of pathogenic importance; however, diagnostic capacities to determine their distribution are lacking in developing countries (38). These other Campylobacter species constitute over 50% of campylobacters isolated at the Red Cross Children’s Hospital, Cape Town, South Africa, for example. (A method termed the Cape Town Protocol is used to isolate Campylobacter species at this facility [39]). This higher incidence is also supported by a 16% isolation rate of Arcobacter species in a 4-month survey from poultry drainage water in Lagos, Nigeria (40).
Antibiotic Resistance in Campylobacter Isolates
Campylobacter enteritis is a self-limiting disease, and antimicrobial therapy is not generally recommended. However, antimicrobial agents are recommended for extraintestinal infections and for treating immunocompromised persons. Erythromycin and ciprofloxacin are drugs of choice (10). The rate of resistance to these drugs is increasing in both developed and developing countries, although the incidence is higher in developing countries. Use of these drugs for infections other than gastroenteritis and self-medication are often the causes of resistance in developing countries; in developed countries, resistance is due to their use in food animals and travel to developing countries. The increase in erythromycin resistance in developed countries is often low and stable at approximately 1% to 2%; this is not true for developing countries (41,42). For example, in 1984, 82% of Campylobacter strains from Lagos, Nigeria, were sensitive to erythromycin; 10 years later, only 20.8% were sensitive (17). In addition, resistance to another macrolide, azithromycin, was found in 7% to 15% of Campylobacter isolates in 1994 and 1995 in Thailand (43). The increasing rate of resistance to the fluoroquinolone, ciprofloxacin limits its clinical usefulness. In Thailand, ciprofloxacin resistance among Campylobacter species increased from zero before 1991 to 84% in 1995 (43). Recent data have shown a marked increase in resistance to quinolones in developed countries (41,42,44–46) (Table 4).
Table 4. Trends in Resistance to Ciprofloxacin by Campylobacter jejuni in selected developed countries up to year 2000.
| Country | Period | Resistance strains (%) | Ref. | |
|---|---|---|---|---|
| Initial | Year 2000 | |||
| Freiburg, Germany | 1992-2000 | 22 | 32 | 41 |
| Styria, Austria | 1996-2000 | 25.2 | 40.2 | 42 |
| England and Wales, UK | 1993-2000 | 10 | 14.8 | 44 |
| Philadelphia, USA | 1995-2000 | <10 | 36 | 45 |
| Oslo, Norway | 1988-2000 | 6.1 | 36 | 46 |
Campylobacter as a Cause of Travelers’ Diarrhea
Travel to a developing country is a risk factor for acquiring Campylobacter-associated diarrhea. The diarrhea is more severe, and strains are associated with antibiotic resistance (47,48). Furthermore, campylobacteriosis acquired abroad contribute to the number of cases reported in developed countries (49). Among Finnish tourists visiting Morocco, the disease was more prevalent in winter months (50).
Clinical Features
The clinical spectrum of Campylobacter enteritis ranges from a watery, nonbloody, noninflammatory diarrhea to a severe inflammatory diarrhea with abdominal pain and fever. Disease is less severe in developing countries than in developed countries (5,6). In developed countries, disease is characterized by bloody stool, fever, and abdominal pain that is often more severe than that observed for Shigella and Salmonella infections. In developing countries the features reported are watery stool, fever, abdominal pain, vomiting, dehydration, and presence of fecal leukocytes; patients are also often underweight and malnourished (12,31,51). In Lagos, Nigeria, Campylobacter enteritis is characterized by a history of watery offensive-smelling stool lasting <5 days (51).
Guillain-Barré Syndrome
Guillain-Barré Syndrome (GBS) is an autoimmune disorder of the peripheral nervous system, which is characterized by acute flaccid paralysis. C. jejuni infection is the most frequently identified infection preceding GBS (52). In the developing world, sporadic GBS cases associated with C. jejuni infection have been reported from Curaçao, China, India, and South Africa (26,53–55). A comparative study between Curaçao and southwest Netherlands indicated that disease in Curaçao was more severe, had a higher incidence of preceding gastroenteritis, and had greater seasonal fluctuation (26). Serotype O:19 is most prevalent worldwide, although other serotypes, such as O:1, O:2, O:57, O:16, O:23, O:37, O:41, and O:44, have been reported (52). C. jejuni strain O:41 appears to be restricted to Cape Town, South Africa, and represents a genetically stable clone (55,56). Detailed studies of the role of GBS in acute flaccid paralysis in developing countries, especially in polio-endemic areas, are needed.
Campylobacter Infection in the Setting of HIV
Campylobacter-associated diarrhea and bacteremia occur in HIV/AIDS patients worldwide. The species isolated include C. jejuni, C. coli, C. upsaliensis, Arcobacter butzleri, Helicobacter fennelliae, and H. cinaedii (57,58). The incidence of clinical manifestations is higher than in HIV-negative patients, with substantial mortality and morbidity. Furthermore, antibiotic resistance and recurrent infections have been observed (59). The incidence of HIV/AIDS is higher in developing countries than in developed countries and contributes substantially to deaths among <5-year-old children in epidemic settings (60). Thus, infants in developing countries are at risk of impaired immunity to Campylobacter enteritis. In addition, HIV/AIDS can increase the number of cases of campylobacteriosis in the adult population in these countries. These observations further support the need for improved understanding of the epidemiology of campylobacteriosis in developing countries.
Immunologic Aspects
In developing countries, such as Bangladesh, Thailand, Central African Republic, and Mexico, healthy children and adults are constantly exposed to Campylobacter antigens in the environment. As a consequence, serum antibodies to Campylobacter species develop very early in life in children in developing countries, and the levels of such antibodies tend to be much higher than those in children in the developed world such as in the United States (61–64). In Nigeria, children who had diarrhea and children who were healthy both had antibodies in their sera that could agglutinate C. jejuni; the difference in antibody responses between these groups of children was not statistically significant (65). Thus, antibody responses alone should be interpreted with caution in diagnosing Campylobacter infections.
In spite of shortcomings in the use of antibodies for diagnosis, increase in the level of anti-flagellar antibody had an inverse correlation with the rates of Campylobacter enteritis in the Central African Republic (63). An age-related relationship in the development of immunity to Campylobacter antigens has also been suggested to account for the age-related declines in the case-to-infection ratio and the period of excretion during the convalescent phase (28,66).
Usually, as age increases, level of antibody tends to increase. At the earliest stages in life (first 6 months), immunoglobulin (Ig) A, IgG, and IgM levels in response to Campylobacter infection are minimal, but thereafter increases are observed in response to infection. The poor serologic response during the first 6 months of life may be due either to a primary response to Campylobacter or to the presence of maternal antibodies via the placenta or breast milk.
Breast-feeding has been reported to play a role in C. jejuni-induced diarrhea. It decreases the number of episodes and the duration of diarrhea (67). In Algeria, exclusively breast-fed infants had fewer symptomatic Campylobacter infections than infants who were both breast-fed and bottle-fed (14).
Results of experimental observations among Mexican children have also shown that immunity to Campylobacter after primary infection may prevent bloody diarrhea from developing and subsequently prevent any disease from manifesting (68). In the developed world, the epidemiology may be different because most cases are usually primary infections with more severe clinical manifestations, greater numbers of people with bloody diarrhea (50%, as opposed to 15% in developing countries), and a more prolonged duration of excretion (approximately 15 days, compared with 7 days in developing countries) (28). The widespread immunity seen among adults in developing countries is absent in adults in developed countries (64).
Sources of Human Campylobacteriosis
Campylobacter infection is hyperendemic in developing countries. The major sources of human infections are environmental contamination and foods. Human-to-human transmission as a result of prolonged convalescent-phase excretion and high population density have also been suggested (5,12), although observations from developed countries show these are less likely factors (2).
Environmental Contamination
Wild birds as well as domestic and companion animals are known reservoirs for Campylobacter species, and shedding of the bacteria from them causes contamination of the environment. C. jejuni and C. coli have been isolated from chickens, goats, sheep, and pigs in developing countries (69,70). Strains isolated from human and chickens were phenotypically and genotypically correlated, confirming that chickens are an important source of human campylobacteriosis in developing countries (36). Poultry is also an important source of campylobacteriosis in developed countries. Extensive epidemiologic investigations have been done in those countries to identify sources of contamination and routes of transmission to humans to facilitate control efforts (71). Risk factors for acquiring campylobacters in developing countries include presence of an animal in the cooking area, uncovered garbage in cooking areas, and lack of piped water (12).
Foods
Campylobacter-contaminated foods—the result of poor sanitation—are an important potential source of infection in humans. For example, campylobacters were isolated from 40% and 77% of retail poultry meat sold in Bangkok, Thailand, and Nairobi, Kenya, respectively (72,73). The serotypes of the organisms isolated in Thailand were similar to those of organisms isolated from humans. In Mexico City, a survey of ready-to-eat roasted chickens showed that they were contaminated with campylobacters (74). In developed countries, risk factors associated with foods include occupational exposure to farm animals, consumption of raw milk or milk products, and unhygienic food preparation practices (2).
Estimates of Impact of Human Campylobacteriosis in Developing Countries
The Disability Adjusted Life Year (DALY) is the basic unit used in Burden of Disease (BoD) methodology to quantify the impact of disease on a population (75). DALYs have been applied in the Dutch population to measure the mean health burden of Campylobacter-associated illness in the period 1990–1995 (76). The mean estimate was 1,400 DALYs per year; the main determinants of health burden were acute gastroenteritis (440 DALYs), gastroenteritis-related mortality (310 DALYs), and residual symptoms of GBS (340 DALYs). Although data on DALYs due to campylobacteriosis in developing countries are not available, diarrhea, which is a clinical manifestation of campylobacteriosis, was one of the top three causes of death and disease in developing countries in 1990 (75). The disease is projected globally to remain one of the top 10 by 2020. (The burden of campylobacteriosis in developing countries may increase by 2020 because HIV is projected to move up to the 10th position from 28th by 2020.) Considering the higher incidence of campylobacteriosis in developing countries, DALYs for the disease in developing countries will likely be higher than those of the Dutch population.
Conclusions
The incidence of human campylobacteriosis is increasing worldwide and has attracted the attention of WHO (http://www1.oecd.org/agr/prog/sum-copenhagen00.htm). Ssubstantial gaps in knowledge about the epidemiology of campylobacteriosis in developing countries still exist. Present reported estimates of incidence are based on isolation rates from laboratory- and community-based studies conducted from 1980 to 1995. When various socioeconomic and health changes in developing countries are taken into account, these values may have changed considerably. Thus, public health awareness about the problem is needed, as are strengthened diagnostic facilities for campylobacteriosis, with a view towards setting up national surveillance programs. Such programs would determine the incidence rates, epidemiologic risk factors, interaction of HIV/AIDS and campylobacteriosis, seasonal variation, current state of resistance to antimicrobial agents, role of species other than C. jejuni and C. coli, and the role of campylobacteriosis in GBS. Collaboration among researchers in developed and developing countries needs to be strengthened, leading to development of regional centers of excellence. Funding organizations should provide incentives for North-South collaborations in Campylobacter research, as is done in other diseases such as malaria and trypanosomiasis that are endemic in some developing countries. All these should contribute to understanding of the global epidemiology of human campylobacteriosis.
Acknowledgments
We thank Henrik C. Wegener and Klaus Stoehrk for their useful suggestions during the preparation of this manuscript.
Biography
Akitoye O. Coker is a Consultant Microbiologist and Professor of Medical Microbiology at the University of Lagos, Nigeria. He pioneered research into Campylobacter enteritis in Lagos, Nigeria.
Suggested citation: Coker AO, Isokpehi RD,* Thomas BN, Amisu KO, and Obi CL. Human Campylobacteriosis in Developing Countries. Emerg Infect Dis. [serial on the Internet]. 2002 Mar [date cited]. Available from http://www.cdc.gov/ncidod/EID/vol8no3/01-0233.htm
1 Portions of this review were presented at the World Health Organization Consultation on the Increasing Incidence of Campylobacteriosis in Humans, Copenhagen, Denmark, November 1-25, 2000. In addition, relevant emerging information from the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, held in Freiburg, Germany, September 1-5, 2001, are included.
References
- 1.Nachamkin I, Blaser MJ. Campylobacter, 2nd edition. Washington: American Society for Microbiology; 2000. [Google Scholar]
- 2.Altekruse SF, Stern NJ, Fields PI, Swerdlow DL. Campylobacter jejuni--an emerging foodborne pathogen. Emerg Infect Dis. 1999;5:28–35. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Tropical disease research: progress 1999-2000. Fifteenth Programme report of the UNDP/World Bank/WHO Special Programme for Research & Training in Tropical Diseases. (TDR/GEN/01.5). Geneva: World Health Organization; 2001. [Google Scholar]
- 4.Lambrechts T, Bryce J, Orinda V. Integrated management of childhood illness: a summary of first experiences. Bull World Health Organ. 1999;77:582–94. [PMC free article] [PubMed] [Google Scholar]
- 5.Taylor DN. Campylobacter infections in developing countries. In: Nachamkin I, Blaser MJ, Tompkins LS, editors. Campylobacter jejuni: Current status and future trends. Washington: American Society for Microbiology; 1992. p.20-30. [Google Scholar]
- 6.Oberhelman RA, Taylor DN. Campylobacter infections in developing countries. In: Nachamkin I, Blaser MJ, editors. Campylobacter, 2nd edition. Washington: American Society for Microbiology; 2000. p.139-53. [Google Scholar]
- 7.Coker AO, Isokpehi RD, Thomas BN, Amisu KO. International collaboration on campylobacters: Experience from Lagos, Nigeria. [Abstract S-05]. In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291 (Suppl 31):168. [PubMed]
- 8.Tauxe RV. Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. In: Nachamkin I, Blaser MJ, Tompkins LS, editors. Campylobacter jejuni: Current and future trends. Washington: American Society for Microbiology; 1992. p.9-12. [Google Scholar]
- 9.de Wit MAS, Koopmans MPG, Kortbeek LM, van Leeuwen NJ, Bartelds AIM, van Duynhoven YTHP. Gastroenteritis in sentinel general practices, the Netherlands. Emerg Infect Dis. 2001;7:82–91. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Engberg J, Aarestrup FM, Taylor DE, Gerner-Smidt P, Nachamkin I. Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance and trends in human isolates. Emerg Infect Dis. 2001;7:24–34. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Frost JA. Current epidemiological issues in human campylobacteriosis. J Appl Microbiol. 2001;90(Suppl):85S–95S. 10.1046/j.1365-2672.2001.01357.x [DOI] [PubMed] [Google Scholar]
- 12.Rao MR, Naficy AB, Savarino SJ, Abu-Elyazeed R, Wierzba TF, Peruski LF, et al. Pathogenicity and convalescent excretion of Campylobacter in rural Egyptian children. Am J Epidemiol. 2001;154:166–73. 10.1093/aje/154.2.166 [DOI] [PubMed] [Google Scholar]
- 13.Wren BW, Linton D, Dorrell N, Karlyshev AV. Post genome analysis of Campylobacter jejuni. J Appl Microbiol. 2001;90(Suppl):36S–44S. 10.1046/j.1365-2672.2001.01352.x [DOI] [PubMed] [Google Scholar]
- 14.Megraud F, Boudraa G, Bessaoud K, Bensid S, Dabis F, Soltana R, et al. Incidence of Campylobacter infection in infants in western Algeria and the possible protective role of breast feeding. Epidemiol Infect. 1990;105:73–8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Koulla-Shiro S, Loe C, Ekoe T. Prevalence of Campylobacter enteritis in children from Yaounde (Cameroon). Cent Afr J Med. 1995;41:91–4. [PubMed] [Google Scholar]
- 16.Gedlu E, Aseffa A. Campylobacter enteritis among children in north-west Ethiopia: a 1-year prospective study. Ann Trop Paediatr. 1996;16:207–12. [DOI] [PubMed] [Google Scholar]
- 17.Coker AO, Adefeso AO. The changing patterns of Campylobacter jejuni/coli in Lagos, Nigeria after ten years. East Afr Med J. 1994;71:437–40. [PubMed] [Google Scholar]
- 18.Lindblom GB, Ahren C, Changalucha J, Gabone R, Kaijser B, Nilsson LA, et al. Campylobacter jejuni/coli and enterotoxigenic Escherichia coli (ETEC) in faeces from children and adults in Tanzania. Scand J Infect Dis. 1995;27:589–93. 10.3109/00365549509047073 [DOI] [PubMed] [Google Scholar]
- 19.Simango C, Nyahanana M. Campylobacter enteritis in children in an urban community. Cent Afr Med J. 1997;43:172–5. [PubMed] [Google Scholar]
- 20.Mangia AH, Duarte AN, Duarte R, Silva LA, Bravo VL, Leal MC. Aetiology of acute diarrhoea in hospitalized children in Rio de Janeiro City, Brazil. J Trop Pediatr. 1993;39:365–7. [DOI] [PubMed] [Google Scholar]
- 21.Ramiro Cruz J, Cano F, Bartlett AV, Mendez H. Infection, diarrhea, and dysentery caused by Shigella species and Campylobacter jejuni among Guatemalan rural children. Pediatr Infect Dis J. 1994;13:216–23. [DOI] [PubMed] [Google Scholar]
- 22.Na'was TE, Abo-Shehada MN. A study of the bacterial and parasitic causes of acute diarrhoea in northern Jordan. J Diarrhoeal Dis Res. 1991;9:305–9. [PubMed] [Google Scholar]
- 23.Albert MJ, Faruque AS, Faruque SM, Sack RB, Mahalanabis D. Case-control study of enteropathogens associated with childhood diarrhea in Dhaka, Bangladesh. J Clin Microbiol. 1999;37:3458–64. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 24.Echeverria P, Taylor DN, Lexsomboon U, Bhaibulaya M, Blacklow NR, Tamura K, et al. Case-control study of endemic diarrheal disease in Thai children. J Infect Dis. 1989;159:543–8. [DOI] [PubMed] [Google Scholar]
- 25.Yamashiro T, Nakasone N, Higa N, Iwanaga M, Insisiengmay S, Phounane T, et al. Etiological study of diarrheal patients in Vientiane, Lao People's Democratic Republic. J Clin Microbiol. 1998;36:2195–9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 26.van Koningsveld R, Rico R, Gerstenbluth I, Schmitz PI, Ang CW, Merkies IS, et al. Gastroenteritis-associated Guillain-Barré syndrome on the Caribbean island Curacao. Neurology. 2001;56:1467–72. [DOI] [PubMed] [Google Scholar]
- 27.Desheng L, Zhixin C, Bolun W. Age distribution of diarrhoeal and healthy children infected with Campylobacter jejuni. J Trop Med Hyg. 1992;95:218–20. [PubMed] [Google Scholar]
- 28.Taylor DN, Perlman DM, Echeverria PD, Lexomboon U, Blaser MJ. Campylobacter immunity and quantitative excretion rates in Thai children. J Infect Dis. 1993;168:754–8. [DOI] [PubMed] [Google Scholar]
- 29.Haq JA, Rahman KM. Campylobacter jejuni as a cause of acute diarrhoea in children: a study at an urban hospital in Bangladesh. J Trop Med Hyg. 1991;94:50–4. [PubMed] [Google Scholar]
- 30.Bichile LS, Saraswati K, Popat UR, Nanivadekar SA, Deodhar LP. Acute Campylobacter jejuni enteritis in 385 hospitalised patients. J Assoc Physicians India. 1992;40:164–6. [PubMed] [Google Scholar]
- 31.Bhadra RK, Lior H, Misra SK, Pal SC, Nair GB. Serotypes & biotypes of Campylobacter jejuni & C. coli from diverse sources in Calcutta. Indian J Med Res. 1989;89:225–8. [PubMed] [Google Scholar]
- 32.Nath G, Shukla BN, Reddy DC, Sanyal SC. A community study on the aetiology of childhood diarrhoea with special reference to Campylobacter jejuni in a semiurban slum of Varanasi, India. J Diarrhoeal Dis Res. 1993;11:165–8. [PubMed] [Google Scholar]
- 33.Alabi SA, Coker AO, Dosunmu-Ogunbi O, Odugbemi T. Biotype and serogroup distribution of Campylobacter isolates from children in Nigeria. J Clin Microbiol. 1986;24:856–8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 34.Georges-Courbot MC, Gouandjika I, Martin PM, Georges AJ. Biotype and Lior serogroup distribution of enteric Campylobacter isolated from children in Bangui (Central African Republic), and comparison with Penner serotypes. Res Microbiol. 1989;140:489–97. 10.1016/0923-2508(89)90070-3 [DOI] [PubMed] [Google Scholar]
- 35.Lastovica AJ, le Roux E, Congi RV, Penner JL. Distribution of sero-biotypes of Campylobacter jejuni and C. coli isolated from paediatric patients. J Med Microbiol. 1986;21:1–5. [DOI] [PubMed] [Google Scholar]
- 36.Adegbola RA, Alabi SA, Akinkuade FO, Coker AO, Odugbemi T. Correlation between human and animal bio-serogroups of Campylobacter isolates in Nigeria. J Trop Med Hyg. 1990;93:280–3. [PubMed] [Google Scholar]
- 37.Smith SI, Olukoya DK, Fox AJ, Coker AO. Deoxyribonucleic acid restriction digest patterns in Campylobacter species: a comparison with Penner serotype. Br J Biomed Sci. 2000;57:137–41. [PubMed] [Google Scholar]
- 38.Vandenberg O, Dediste A, Vlaes L, Ebraert A, Retore P, Douat N, et al. Prevalence and clinical features of non jejuni/coli Campylobacter species and related organisms in stool specimens. [Abstract P-22]. In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291(Suppl 31):144. [PubMed]
- 39.le Roux E, Lastovica AJ. The Cape Town Protocol: how to isolate the most campylobacters for your dollar, pound, franc, yen, etc. In: Lastovica AJ, Newell DG, Lastovica EE, editors. Proceedings of the 9th International Workshop on Campylobacter, Helicobacter and Related Organisms, Cape Town, South Africa Sept 15-19 1997. Cape Town: Institute of Child Health; 1998. p. 30-33.
- 40.Amisu KO, Coker AO, Isokpehi RD. First isolation of aerotolerant Campylobacter (Arcobacter) strains in Nigeria. [Abstract P-18] In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291 (Suppl 31):143. [PubMed]
- 41.Steinbrückner B, Ruberg F, Vetter-Knoll M, Kist M. Antimicrobial susceptibility of Campylobacter jejuni and Campylobacter coli isolated in Freiburg from 1992 to 2000. [Abstract B-12] In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291 (Suppl 31):8. [PubMed]
- 42.Feierl G, Wagner U, Sixl B, Grisold A, Daghofer E, Marth E. Epidemiology of campylobacteriosis and development of resistance in Styria, Austria. [Abstract B-15]. In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291 (Suppl 31):9.
- 43.Hoge CW, Gambel JM, Srijan A, Pitarangsi C, Echeverria P. Trends in antibiotic resistance among diarrheal pathogens isolated in Thailand over 15 years. Clin Infect Dis. 1998;26:341–5. 10.1086/516303 [DOI] [PubMed] [Google Scholar]
- 44.Wickin HV, Thwaites RT, Frost JA. Drug resistance in Campylobacter species in England and Wales, 1993-2001. [Abstract B-05] In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001; 291 (Suppl 31):6. [PubMed]
- 45.Nachamkin I, Ung H, Li M. Fluoroquinolone resistant Campylobacter jejuni in Philadelphia, 1982-2000. [Abstract B-06]. In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291 (Suppl 31):6. [PubMed]
- 46.Melby KK, Mannsåker T. Antibiotic sensitivity pattern of Campylobacter spp recovered over a ten year period from patients residing in Oslo Area. [Abstract B-17]. In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291 (Suppl 31):9. [PubMed]
- 47.Gallardo F, Gascon J, Ruiz J, Corachan M, Jimenez de Anta M, Vila J. Campylobacter jejuni as a cause of traveler's diarrhea: clinical features and antimicrobial susceptibility. J Travel Med. 1998;5:23–6. 10.1111/j.1708-8305.1998.tb00451.x [DOI] [PubMed] [Google Scholar]
- 48.Shlim DR, Hoge CW, Rajah R, Scott RM, Pandy P, Echeverria P. Persistent high risk of diarrhea among foreigners in Nepal during the first 2 years of residence. Clin Infect Dis. 1999;29:613–6. 10.1086/598642 [DOI] [PubMed] [Google Scholar]
- 49.Smith KE, Besser JM, Hedberg CW, Leano FT, Bender JB, Wicklund JH, et al. Quinolone-resistant Campylobacter jejuni infections in Minnesota, 1992-1998. N Engl J Med. 1999;340:1525–32. 10.1056/NEJM199905203402001 [DOI] [PubMed] [Google Scholar]
- 50.Mattila L, Siitonen A, Kyronseppa H, Simula I, Oksanen P, Stenvik M, et al. Seasonal variation in etiology of travelers' diarrhea. Finnish-Moroccan Study Group. J Infect Dis. 1992;165:385–8. [DOI] [PubMed] [Google Scholar]
- 51.Coker AO, Dosunmu-Ogunbi O. Gastroenteritis due to Campylobacter jejuni in Lagos, Nigeria. Cent Afr J Med. 1985;31:72–4. [PubMed] [Google Scholar]
- 52.Nachamkin I, Allos BM, Ho T. Campylobacter species and Guillain-Barré syndrome. Clin Microbiol Rev. 1998;11:555–67. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 53.Ho TW, Mishu B, Li CY, Gao CY, Cornblath DR, Griffin JW, et al. Guillain-Barré syndrome in northern China. Relationship to Campylobacter jejuni infection and anti-glycolipid antibodies. Brain. 1995;118:597–605. 10.1093/brain/118.3.597 [DOI] [PubMed] [Google Scholar]
- 54.Hariharan H, Naseema K, Kumaran C, Shanmugam J, Nair MD, Radhakrishnan K. Detection of Campylobacter jejuni/C. coli infection in patients with Guillain-Barré syndrome by serology and culture. New Microbiol. 1996;19:267–71. [PubMed] [Google Scholar]
- 55.Lastovica AJ, Goddard EA, Argent AC. Guillain-Barré syndrome in South Africa associated with Campylobacter jejuni O:41 strains. J Infect Dis. 1997;176(Suppl 2):S139–43. 10.1086/513796 [DOI] [PubMed] [Google Scholar]
- 56.Wassenaar TM, Fry BN, Lastovica AJ, Wagenaar JA, Coloe PJ, Duim B. Genetic characterization of Campylobacter jejuni O:41 isolates in relation with Guillain-Barre syndrome. J Clin Microbiol. 2000;38:874–6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 57.Quinn TC. Diversity of Campylobacter species and its impact on patients infected with human immunodeficiency virus. Clin Infect Dis. 1997;24:1114–7. 10.1086/513645 [DOI] [PubMed] [Google Scholar]
- 58.Lastovica AJ, Mitchie C, Maartens G. Campylobacter infection in HIV+ South African children and adults. [Abstract Q-01]. In: Hacker J, editor. Abstracts of scientific presentations of the 11th International Workshop on Campylobacter, Helicobacter and Related Organisms, Freiburg, Germany, Sept 1-5, 2001. Int J Med Microbiol 2001;291 (Suppl 31):151. [PubMed]
- 59.Germani Y, Minssart P, Vohito M, Yassibanda S, Glaziou P, Hocquet D, et al. Etiologies of acute, persistent, and dysenteric diarrheas in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus. Am J Trop Med Hyg. 1998;59:1008–14. [DOI] [PubMed] [Google Scholar]
- 60.Adetunji J. Trends in under-5 mortality rates and the HIV/AIDS epidemic. Bull World Health Organ. 2000;78:1200–6. [PMC free article] [PubMed] [Google Scholar]
- 61.Blaser MJ, Black RE, Duncan DJ, Amer J. Campylobacter jejuni-specific serum antibodies are elevated in healthy Bangladeshi children. J Clin Microbiol. 1985;21:164–7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 62.Blaser MJ, Taylor DN, Echeverria P. Immune response to Campylobacter jejuni in a rural community in Thailand. J Infect Dis. 1986;153:249–54. [DOI] [PubMed] [Google Scholar]
- 63.Martin PM, Mathiot J, Ipero J, Kirimat M, Georges AJ, Georges-Courbot MC. Immune response to Campylobacter jejuni and Campylobacter coli in a cohort of children from birth to 2 years of age. J Clin Microbiol. 1989;57:2542–6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 64.Blaser MJ. Epidemiologic and clinical features of Campylobacter jejuni infections. J Infect Dis. 1997;176(Suppl. 2):S103–5. 10.1086/513780 [DOI] [PubMed] [Google Scholar]
- 65.Obi CL, Coker AO. Campylobacter jejuni antibody in patients with diarrhoea and asymptomatic individuals in Lagos, Nigeria. Cent Afr J Med. 1989;66:276–9. [PubMed] [Google Scholar]
- 66.Taylor DN, Echeverria P, Pitarangsi C, Seriwatana J, Bodhidatta L, Blaser MJ. Influence of strain characteristics and immunity on the epidemiology of Campylobacter infections in Thailand. J Clin Microbiol. 1988;26:863–8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 67.Ruiz-Palacious GM, Calva JJ, Pickering LK, Lopez-Vidal Y, Volkow P, Pezzarossi H, et al. Protection of breast-fed infants against Campylobacter diarrhea by antibodies in human milk. J Pediatr. 1990;116:707–13. 10.1016/S0022-3476(05)82652-6 [DOI] [PubMed] [Google Scholar]
- 68.Calva JJ, Ruiz-Palacious GM, Lopez-Vidal AB, Ramos A, Bojalil R. Cohort study of intestinal infection with Campylobacter in Mexican children. Lancet. 1988;I:503–6. 10.1016/S0140-6736(88)91297-4 [DOI] [PubMed] [Google Scholar]
- 69.Coker AO, Isokpehi RD, Thomas BN, Fagbenro-Beyioku AF, Omilabu SA. Zoonotic infections in Nigeria: overview from a medical perspective. Acta Trop. 2000;76:59–63. 10.1016/S0001-706X(00)00091-7 [DOI] [PubMed] [Google Scholar]
- 70.Raji MA, Adekeye JO, Kwaga JK, Bale JO. Bioserogroups of Campylobacter species isolated from sheep in Kaduna State, Nigeria. Small Rumin Res. 2000;37:215–21. 10.1016/S0921-4488(00)00125-5 [DOI] [PubMed] [Google Scholar]
- 71.Pattison M. Practical intervention strategies for Campylobacter. J Appl Microbiol. 2001;90(Suppl):121S–5S. 10.1046/j.1365-2672.2001.01360.x [DOI] [PubMed] [Google Scholar]
- 72.Rasrinaul L, Suthienkul O, Echeverria PD, Taylor DN, Seriwatana J, Bangtrakulnonth A, et al. Foods as a source of enteropathogens causing childhood diarrhea in Thailand. Am J Trop Med Hyg. 1988;39:97–102. [DOI] [PubMed] [Google Scholar]
- 73.Osano O, Arimi SM. Retail poultry and beef as sources of Campylobacter jejuni. East Afr Med J. 1999;76:141–3. [PubMed] [Google Scholar]
- 74.Quinones-Ramirez EI, Vazquez-Salinas C, Rodas-Suarez OR, Ramos-Flores MO, Rodriguez-Montano R. Frequency of isolation of Campylobacter from roasted chicken samples from Mexico City. J Food Prot. 2000;63:117–9. [DOI] [PubMed] [Google Scholar]
- 75.Murray CJL, Lopez AD, eds. The global burden of disease: a comprehensive assessment of mortality and disability from diseases, injuries and risk factors in 1990 and projected to 2020. (Global Burden of Disease and Injury Series, Vol. I). Cambridge (MA): Harvard School of Public Health on behalf of the World Health Organization and the World Bank; 1996. [Google Scholar]
- 76.Havelaar AH, de Wit MA, van Koningsveld R, van Kempen E. Health burden in the Netherlands due to infection with thermophilic Campylobacter spp. Epidemiol Infect. 2000;125:505–22. 10.1017/S0950268800004933 [DOI] [PMC free article] [PubMed] [Google Scholar]