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. 1979 Nov;10(5):708–711. doi: 10.1128/jcm.10.5.708-711.1979

Evaluation and comparison of two assays for detection of immunity to rubella infection.

J P Brody, J H Binkley, S A Harding
PMCID: PMC273252  PMID: 397225

Abstract

Two commercially available rapid screening tests, Rubacell (Abbott Laboratories; passive hemagglutination) and FIAX (International Diagnostic Technology; indirect immunofluorescence) were compared with a standard hemagglutination inhibition assay for detection of immunity to rubella infection. In tests of approximately 300 sera, both rapid assays were specific and sensitive and showed a high predictive value of a positive result. Within-run reproducibility studies were excellent for both tests; however, Rubacell was superior to FIAX with respect to time-cost analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleton P. N., Macrae A. D. Comparison of radial haemolysis with haemagglutination inhibition in estimating rubella antibody. J Clin Pathol. 1978 May;31(5):479–482. doi: 10.1136/jcp.31.5.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROWN G. C., MAASSAB H. F., VERONELLI J. A., FRANCIS T. J., Jr RUBELLA ANTIBODIES IN HUMAN SERUM: DETECTION BY THE INDIRECT FLUORESCENT ANTIBODY TECHNIC. Science. 1964 Aug 28;145(3635):943–945. doi: 10.1126/science.145.3635.943. [DOI] [PubMed] [Google Scholar]
  4. Grandien M., Norrby E. Evaluation of an immunodiffusion test for screening of rubella immunity. Acta Pathol Microbiol Scand C. 1976 Apr;84(2):153–160. doi: 10.1111/j.1699-0463.1976.tb00014.x. [DOI] [PubMed] [Google Scholar]
  5. Schmidt N. J., Lennette E. H. Variables of the rubella hemagglutination-inhibition test system and their effect on antigen and antibody titers. Appl Microbiol. 1970 Mar;19(3):491–504. doi: 10.1128/am.19.3.491-504.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stewart G. L., Parkman P. D., Hopps H. E., Douglas R. D., Hamilton J. P., Meyer H. M., Jr Rubella-virus hemagglutination-inhibition test. N Engl J Med. 1967 Mar 9;276(10):554–557. doi: 10.1056/NEJM196703092761006. [DOI] [PubMed] [Google Scholar]
  7. Sugishita C., O'Shea S., Best J. M., Banatvala J. E. Rubella serology by solid-phase radioimmunoassay: its potential for screening programmes. Clin Exp Immunol. 1978 Jan;31(1):50–54. [PMC free article] [PubMed] [Google Scholar]

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