Abstract
In an outbreak of acute gastroenteritis in an institution for the mentally retarded at Otofuke, Hokkaido, Japan, virus-like particles were observed by electron microscopy in five of seven stool specimens from the patients. The particles had 10 rod-shaped and 10 round projections or capsomeres on the periphery, measured 35 to 40 nm in diameter, and had a buoyant density of 1.35 to 1.37 g/ml in cesium chloride. Attempts to culture these particles in tissue culture or in mouse brain were unsuccessful. Immune electron miscroscopy performed with the virus from the patients as antigen demonstrated significant serological responses in all patients examined. Antigenic similarity of the virus particles obtained from five patients was also confirmed by immune electron microscopy with the paired acute- and convalescent-phase sera of one of the patients. Furthermore, in immune electron micrsocopy these particles appeared to have no antigenic relationship to three candidate viruses for gastroenteritis so far reported: the Norwalk agent, the W agent, and the calicivirus-like particle. These results suggested the possibility that this agent, tentatively designated as the Otofuke agent, might be a new candidate virus for gastroenteritis.
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Selected References
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- Appleton H., Buckley M., Thom B. T., Cotton J. L., Henderson S. Virus-like particles in winter vomiting disease. Lancet. 1977 Feb 19;1(8008):409–411. doi: 10.1016/s0140-6736(77)92614-9. [DOI] [PubMed] [Google Scholar]
- Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
- Caul E. O., Clarke S. K. Coronavirus propagated from patient with non-bacterial gastroenteritis. Lancet. 1975 Nov 15;2(7942):953–954. doi: 10.1016/S0140-6736(75)90363-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clarke S. K., Cook G. T., Egglestone S. I., Hall T. S., Miller D. L., Reed S. E., Rubenstein D., Smith A. J., Tyrrell D. A. A virus from epidemic vomiting disease. Br Med J. 1972 Jul 8;3(5818):86–89. doi: 10.1136/bmj.3.5818.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
- EICHENWALD H. F., ABABIO A., ARKY A. M., HARTMAN A. P. Epidemic diarrhea in premature and older infants caused by ECHO virus type 18. J Am Med Assoc. 1958 Mar 29;166(13):1563–1566. doi: 10.1001/jama.1958.02990130023005. [DOI] [PubMed] [Google Scholar]
- Hamilton J. R., Gall D. G., Kerzner B., Butler D. G., Middleton P. J. Recent developments in viral gastroenteritis. Pediatr Clin North Am. 1975 Nov;22(4):747–755. doi: 10.1016/S0031-3955(16)33205-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kapikian A. Z., Gerin J. L., Wyatt R. G., Thornhill T. S., Chanock R. M. Density in cesium chloride of the 27 nm "8FIIa" particle associated with acute infectious nonbacterial gastroenteritis: determination by ultra-centrifugation and immune electron microscopy. Proc Soc Exp Biol Med. 1973 Mar;142(3):874–877. doi: 10.3181/00379727-142-37135. [DOI] [PubMed] [Google Scholar]
- Kapikian A. Z., Kim H. W., Wyatt R. G., Cline W. L., Arrobio J. O., Brandt C. D., Rodriguez W. J., Sack D. A., Chanock R. M., Parrott R. H. Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children. N Engl J Med. 1976 Apr 29;294(18):965–972. doi: 10.1056/NEJM197604292941801. [DOI] [PubMed] [Google Scholar]
- Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madeley C. R., Cosgrove B. P. Letter: Caliciviruses in man. Lancet. 1976 Jan 24;1(7952):199–200. doi: 10.1016/s0140-6736(76)91309-x. [DOI] [PubMed] [Google Scholar]
- Madeley C. R., Cosgrove B. P. Letter: Viruses in infantile gastroenteritis. Lancet. 1975 Jul 19;2(7925):124–124. doi: 10.1016/s0140-6736(75)90020-3. [DOI] [PubMed] [Google Scholar]
- Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Am J Dis Child. 1977 Jul;131(7):733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
- Morris C. A., Flewett T. H., Bryden A. S., Davies H. Epidemic viral enteritis in a long-stay children's ward. Lancet. 1975 Jan 4;1(7897):4–5. doi: 10.1016/s0140-6736(75)92370-3. [DOI] [PubMed] [Google Scholar]
- Parrino T. A., Schreiber D. S., Trier J. S., Kapikian A. Z., Blacklow N. R. Clinical immunity in acute gastroenteritis caused by Norwalk agent. N Engl J Med. 1977 Jul 14;297(2):86–89. doi: 10.1056/NEJM197707142970204. [DOI] [PubMed] [Google Scholar]
- Studdert M. J. Caliciviruses. Brief review. Arch Virol. 1978;58(3):157–191. doi: 10.1007/BF01317600. [DOI] [PubMed] [Google Scholar]
- Thornhill T. S., Wyatt R. G., Kalica A. R., Dolin R., Chanock R. M., Kapikian A. Z. Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis. J Infect Dis. 1977 Jan;135(1):20–27. doi: 10.1093/infdis/135.1.20. [DOI] [PubMed] [Google Scholar]