Abstract
Approximately 150 sound root surfaces in 44 subjects prone to root surface caries were sampled longitudinally to determine the microbial flora associated with root caries initiation. During the first 16 months of the study, a comparison of Streptococcus mutans recovery was made by using three bacteriological media: mitis-salivarius agar (MSA), mitis-salivarius-bacitracin-sucrose agar (MSB), and a partially selective mannitol-containing broth. Total streptococcal and S. mutans populations were found to be much lower than in previous reports. MSB was more selective; S. mutans was detected in many samples when its numbers were too low for isolation on MSA. However, recovery of S. mutans was greater on MSA than on MSB for most samples yielding colonies on both media. Mannitol-containing broth used as an enrichment medium yielded the highest frequency of S. mutans isolation among the three media.
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Selected References
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- Duchin S., van Houte J. Relationship of Streptococcus mutans and lactobacilli to incipient smooth surface dental caries in man. Arch Oral Biol. 1978;23(9):779–786. doi: 10.1016/0003-9969(78)90155-3. [DOI] [PubMed] [Google Scholar]
- Emilson C. G., Bratthall D. Growth of Streptococcus mutans on various selective media. J Clin Microbiol. 1976 Jul;4(1):95–98. doi: 10.1128/jcm.4.1.95-98.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gold O. G., Jordan H. V., Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol. 1973 Nov;18(11):1357–1364. doi: 10.1016/0003-9969(73)90109-x. [DOI] [PubMed] [Google Scholar]
- Hill P. E., Knox K. W., Schamschula R. G., Tabua J. The identification and enumeration of actinomyces from plaque of New Guinea indigenes. Caries Res. 1977;11(6):327–335. doi: 10.1159/000260287. [DOI] [PubMed] [Google Scholar]
- Jordan H. V., Hammond B. F. Filamentous bacteria isolated from human root surface caries. Arch Oral Biol. 1972 Sep;17(9):1333–1342. doi: 10.1016/0003-9969(72)90166-5. [DOI] [PubMed] [Google Scholar]
- Little W. A., Korts D. C., Thomson L. A., Bowen W. H. Comparative recovery of Streptococcus mutans on ten isolation media. J Clin Microbiol. 1977 Jun;5(6):578–583. doi: 10.1128/jcm.5.6.578-583.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson P. J., Shapiro I. M. Effect of diphosphonates on root resorption. J Dent Res. 1976 Jan-Feb;55(1):166–166. doi: 10.1177/00220345760550011201. [DOI] [PubMed] [Google Scholar]
- Staat R. H. Inhibition of Streptococcus mutans strains by different mitis-salivarius agar preparations. J Clin Microbiol. 1976 Mar;3(3):378–380. doi: 10.1128/jcm.3.3.378-380.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sumney D. L., Jordan H. V. Characterization of bacteria isolated from human root surface carious lesions. J Dent Res. 1974 Mar-Apr;53(2):343–351. doi: 10.1177/00220345740530022701. [DOI] [PubMed] [Google Scholar]
- Syed S. A., Loesche W. J. Efficiency of various growth media in recovering oral bacterial flora from human dental plaque. Appl Microbiol. 1973 Oct;26(4):459–465. doi: 10.1128/am.26.4.459-465.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Syed S. A., Loesche W. J., Pape H. L., Jr, grenier E. Predominant cultivable flora isolated from human root surface caries plaque. Infect Immun. 1975 Apr;11(4):727–731. doi: 10.1128/iai.11.4.727-731.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Syed S. A., Loesche W. J. Survival of human dental plaque flora in various transport media. Appl Microbiol. 1972 Oct;24(4):638–644. doi: 10.1128/am.24.4.638-644.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]