Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Mar;11(3):249–255. doi: 10.1128/jcm.11.3.249-255.1980

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci.

M Slifkin, G R Pouchet-Melvin
PMCID: PMC273373  PMID: 7381001

Abstract

Three beta-streptococci serogrouping kits, Phadebact, SeroSTAT, and Streptex, were evaluated as to their sensitivity, accuracy, and suitability as methods for serogrouping streptococci in a clinical microbiology laboratory. The majority of the primary isolates examined by the various methods associated with each of the three kits were correctly identified. The Streptex direct mixed-culture procedure was more often associated with the observation of cross-reactivity than with the direct procedures of the other two kits which did not employ mixed growth cultures. Furthermore, the Streptex kit was associated with more false-negative responses than those determined by the other two kits under evaluation. These results appeared to be due to the relatively poor sensitivity of the Streptex grouping reagents. The Streptex test procedures required more labor than the other kit procedures, requiring a 1-h enzymatic extraction step for the release of the group antigens. The SeroSTAT kit provided only a direct procedure and, thus, is limited in its application. The Phadebact procedures were the most versatile by providing not only a direct and a 24-h grouping procedure, but also by including a 4-h method that may be employed as required by the clinical microbiologist.

Full text

PDF
249

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arvilommi H. Grouping of beta-haemolytic streptococci by using coagglutination, precipitation or bacitracin sensitivity. Acta Pathol Microbiol Scand B. 1976 Apr;84(2):79–84. doi: 10.1111/j.1699-0463.1976.tb01906.x. [DOI] [PubMed] [Google Scholar]
  2. Arvilommi H., Uurasmaa O., Nurkkala A. Rapid identification of group A, B, C and G beta-haemolytic Streptococci by a modification of the co-agglutination technique. Comparison of results obtained by co-agglutination, fluorescent antibody test, counterimmunoelectrophoresis, and precipitin technique. Acta Pathol Microbiol Scand B. 1978 Apr;86(2):107–111. doi: 10.1111/j.1699-0463.1978.tb00017.x. [DOI] [PubMed] [Google Scholar]
  3. Christensen P., Kahlmeter G., Jonsson S., Kronvall G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun. 1973 Jun;7(6):881–885. doi: 10.1128/iai.7.6.881-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ederer G. M., Herrmann M. M., Bruce R., Matsen J. M., Chapman S. S. Rapid extraction method with pronase B for grouping beta-hemolytic streptococci. Appl Microbiol. 1972 Feb;23(2):285–288. doi: 10.1128/am.23.2.285-288.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edwards E. A., Larson G. L. New method of grouping beta-hemolytic streptococci directly on sheep blood agar plates by coagglutination of specifically sensitized protein A-containing staphylococci. Appl Microbiol. 1974 Dec;28(6):972–976. doi: 10.1128/am.28.6.972-976.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Facklam R. R., Padula J. F., Thacker L. G., Wortham E. C., Sconyers B. J. Presumptive identification of group A, B, and D streptococci. Appl Microbiol. 1974 Jan;27(1):107–113. doi: 10.1128/am.27.1.107-113.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farrell B., Amirak I. Agglutination grouping of Streptococci. Lancet. 1976 Nov 13;2(7994):1082–1082. doi: 10.1016/s0140-6736(76)90991-0. [DOI] [PubMed] [Google Scholar]
  8. Finch R. G., Phillips I. Serological grouping of streptococci by a slide coagglutination method. J Clin Pathol. 1977 Feb;30(2):168–170. doi: 10.1136/jcp.30.2.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hahn G., Nyberg I. Identification of streptococcal groups A,B,C, and G by slide co-agglutination of antibody-sensitized protein A-containing staphylococci. J Clin Microbiol. 1976 Jul;4(1):99–101. doi: 10.1128/jcm.4.1.99-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kirkegaard M. K., Field C. R. Rapid slide coagglutination test for identifying and typing group B streptococci. J Clin Microbiol. 1977 Sep;6(3):266–270. doi: 10.1128/jcm.6.3.266-270.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lim D. V., Smith R. D., Day S. Evaluation of an improved rapid coagglutination method for the serological grouping of beta-hemolytic streptococci. Can J Microbiol. 1979 Jan;25(1):40–43. doi: 10.1139/m79-006. [DOI] [PubMed] [Google Scholar]
  12. Lue Y. A., Howit I. P., Ellner P. D. Rapid grouping of beta-hemolytic streptococci by latex agglutination. J Clin Microbiol. 1978 Sep;8(3):326–328. doi: 10.1128/jcm.8.3.326-328.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RANTZ L. A., RANDALL E. Use of autoclaved extracts of hemolytic streptococci for serological grouping. Stanford Med Bull. 1955 May;13(2):290–291. [PubMed] [Google Scholar]
  14. Rosner R. Laboratory evaluation of a rapid four-hour serological grouping of groups A,B,C, and G beta-streptococci by the Phadebact streptococcus test. J Clin Microbiol. 1977 Jul;6(1):23–26. doi: 10.1128/jcm.6.1.23-26.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Slifkin M., Engwall C., Pouchet G. R. Direct-plate serological grouping of beta-hemolytic streptococci from primary isolation plates with the Phadebact streptococcus test. J Clin Microbiol. 1978 Apr;7(4):356–360. doi: 10.1128/jcm.7.4.356-360.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slifkin M., Pouchet G. R. Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator. J Clin Microbiol. 1977 Jan;5(1):15–19. doi: 10.1128/jcm.5.1.15-19.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Watson B. K., Moellering R. C., Jr, Kunz L. J. Identification of streptococci: use of lysozyme and Streptomyces albus filtrate in the preparation of extracts for Lancefield grouping. J Clin Microbiol. 1975 Mar;1(3):274–278. doi: 10.1128/jcm.1.3.274-278.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES