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. 1980 Apr;11(4):363–369. doi: 10.1128/jcm.11.4.363-369.1980

WSJM, a simple chemically defined medium for growth of Neisseria gonorrhoeae.

T P Wong, R K Shockley, K H Johnston
PMCID: PMC273405  PMID: 6768768

Abstract

The growth requirements of 135 clinical isolates of Neisseria gonorrhoeae and six American Type Culture Collection reference strains were examined by using a simple chemically defined medium known as Wong-Shockley-Johnston medium, WSJM. The simple liquid medium supported growth of gonococci from an inoculum of 2 X 10(6) colony-forming units ml-1 and yielded 10(10) colony-forming units ml-1 in 10 h in the absence of CO2. Scale-up experiments with the complete medium yielded 5 to 10 g, wet weight, of cells per liter. The complete medium was stable upon storage at 5 degrees C and after lyophilization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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