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. 1980 Apr;11(4):380–384. doi: 10.1128/jcm.11.4.380-384.1980

Bacterial antigen detection in body fluids: methods for rapid antigen concentration and reduction of nonspecific reactions.

S O Doskeland, B P Berdal
PMCID: PMC273408  PMID: 7372801

Abstract

We sought procedures which would allow a rapid concentration in high yield of bacterial antigens from tissue fluids of patients and which could be applied also to protein-rich fluids like serum. Ethanol precipitation at a subzero temperature with albumin added as an antigen coprecipitant made it possible to achieve a more than 20-fold concentration of antigen in 15 min and a 200-fold concentration in 45 min. Heat-stable antigens could be concentrated from protein-rich fluids (like serum) after the sample had been deproteinized by boiling. Such heating (100 degrees C, 3 min) also liberated bacterial polysaccharides from antibody complexes and elminated the nonspecific interference of serum in enzyme-linked immunosorbent assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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