Abstract
The cellular fatty acid composition of 29 human and animal isolates of Campylobacter fetus was determined with gas-liquid chromatography. All strains contained small amounts of 3-hydroxytetradecanoic acid, suggesting the presence of lipid A. Each of 13 different blood or stool isolates of C. fetus subsp. jejuni obtained from humans or fowl contained a 19-carbon cyclopropane acid which was not present in C. fetus subsp. fetus (6 strains from cattle) or C. fetus subsp. intestinalis (10 strains from humans and cattle). These findings indicate that C. fetus subsp. jejuni can be rapidly differentiated from other C. fetus by gas-liquid chromatography analysis of cellular fatty acids.
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- Blaser M. J., Berkowitz I. D., LaForce F. M., Cravens J., Reller L. B., Wang W. L. Campylobacter enteritis: clinical and epidemiologic features. Ann Intern Med. 1979 Aug;91(2):179–185. doi: 10.7326/0003-4819-91-2-179. [DOI] [PubMed] [Google Scholar]
- Brian B. L., Gardner E. W. A simple procedure for detecting the presence of cyclopropane fatty acids in bacterial lipids. Appl Microbiol. 1968 Apr;16(4):549–552. doi: 10.1128/am.16.4.549-552.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Butzler J. P., Skirrow M. B. Campylobacter enteritis. Clin Gastroenterol. 1979 Sep;8(3):737–765. [PubMed] [Google Scholar]
- Dees S. B., Moss C. W. Identification of Achromobacter species by cellular fatty acids and by production of keto acids. J Clin Microbiol. 1978 Jul;8(1):61–66. doi: 10.1128/jcm.8.1.61-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Humphreys G. O., Hancock I. C., Meadow P. M. Synthesis of the hydroxyacids in lipid A of Pseudomonas aeruginosa. J Gen Microbiol. 1972 Jul;71(2):221–230. doi: 10.1099/00221287-71-2-221. [DOI] [PubMed] [Google Scholar]
- Lambert M. S., Moss C. W. Degradation of bacterial cyclopropane acids with boron trihalide reagents. Microbios. 1977;18(71):51–58. [PubMed] [Google Scholar]
- Moss C. W., Dees S. B. Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens. J Clin Microbiol. 1976 Dec;4(6):492–502. doi: 10.1128/jcm.4.6.492-502.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moss C. W., Lambert M. A., Merwin W. H. Comparison of rapid methods for analysis of bacterial fatty acids. Appl Microbiol. 1974 Jul;28(1):80–85. doi: 10.1128/am.28.1.80-85.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RYHAGE R., STENHAGEN E. Mass spectrometry in lipid research. J Lipid Res. 1960 Oct;1:361–390. [PubMed] [Google Scholar]
- Rettig P. J. Campylobacter infections in human beings. J Pediatr. 1979 Jun;94(6):855–864. doi: 10.1016/s0022-3476(79)80202-4. [DOI] [PubMed] [Google Scholar]
- Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smibert R. M. The genus Campylobacter. Annu Rev Microbiol. 1978;32:673–709. doi: 10.1146/annurev.mi.32.100178.003325. [DOI] [PubMed] [Google Scholar]
- Tornabene T. G., Ogg J. E. Chromatographic studies of the lipid components of Vibrio fetus. Biochim Biophys Acta. 1971 Jul 13;239(2):133–141. doi: 10.1016/0005-2760(71)90159-7. [DOI] [PubMed] [Google Scholar]