Abstract
Coagglutination was compared with counterimmunoelectrophoresis (CIE) for sensitivity and specificity in the detection of pneumococcal antigens in sputum. Initial sputum samples from patients with pneumococcal pneumonia (less than 12 h of antibiotic therapy) were positive for antigens in 37 of 44 cases (84%) by either test. There was a decline in the number of positive results with sputum samples obtained during continuing antibiotic therapy, but the decline was greater with CIE (only 29% of samples were positive at 3 days of therapy) than with coagglutination (61% of samples were positive at 3 days of therapy) (P less than 0.05). Sputum from 3 of 11 patients (27%) and from 2 of 11 patients (18%) with nonpneumococcal pneumonia was positive for pneumococcal antigens by CIE and coagglutination, respectively, indicating a similar degree of non-specificity. Coagglutination produced the same results as CIE with sputum from patients with chronic bronchitis but without pneumonia; 9 of 23 of these patients were positive. Coagglutination was simpler to perform than CIE and required only a fraction (about 1/30) of the antiserum required for CIE. These advantages, plus the greater sensitivity of coagglutination with sputum samples obtained during antibiotic therapy, suggest that coagglutination is preferable to CIE.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barrett-Connor E. The nonvalue of sputum culture in the diagnosis of pneumococcal pneumonia. Am Rev Respir Dis. 1971 Jun;103(6):845–848. doi: 10.1164/arrd.1971.103.6.845. [DOI] [PubMed] [Google Scholar]
- Coonrod J. D., Rytel M. W. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. II. Etiologic diagnosis of pneumococcal pneumonia. J Lab Clin Med. 1973 May;81(5):778–786. [PubMed] [Google Scholar]
- Kenny G. E., Wentworth B. B., Beasley R. P., Foy H. M. Correlation of circulating capsular polysaccharide with bacteremia in pneumococcal pneumonia. Infect Immun. 1972 Oct;6(4):431–437. doi: 10.1128/iai.6.4.431-437.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leach R. P., Coonrod J. D. Detection of penumococcal antigens in the sputum in pneumococcal pneumonia. Am Rev Respir Dis. 1977 Nov;116(5):847–851. doi: 10.1164/arrd.1977.116.5.847. [DOI] [PubMed] [Google Scholar]
- Miller J., Sande M. A., Gwaltney J. M., Jr, Hendley J. O. Diagnosis of pneumococcal pneumonia by antigen detection in sputum. J Clin Microbiol. 1978 May;7(5):459–462. doi: 10.1128/jcm.7.5.459-462.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perlino C. A., Shulman J. A. Detection of pneumococcal polysaccharide in the sputum of patients with pneumococcal pneumonia by counterimmunoelectrophoresis. J Lab Clin Med. 1976 Mar;87(3):496–502. [PubMed] [Google Scholar]
- Spencer R. C., Philp J. R. Effect of previous antimicrobial therapy on bacteriological findings in patients with primary pneumonia. Lancet. 1973 Aug 18;2(7825):349–350. doi: 10.1016/s0140-6736(73)93192-9. [DOI] [PubMed] [Google Scholar]
- Tugwell P., Greenwood B. M. Pneumococcal antigen in lobar pneumonia. J Clin Pathol. 1975 Feb;28(2):118–123. doi: 10.1136/jcp.28.2.118. [DOI] [PMC free article] [PubMed] [Google Scholar]