Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Jun;11(6):600–603. doi: 10.1128/jcm.11.6.600-603.1980

Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.

T Honda, S Chearskul, Y Takeda, T Miwatani
PMCID: PMC273468  PMID: 7430330

Abstract

A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The sensitivity of the modified Elek test was almost the same as that of the Kanagawa phenomenon on Wagatsuma medium, and the results of both reactions correlated very well. The immuno-halo test was a little less sensitive than the modified Elek test. Since the Kanagawa phenomenon on Wagatsuma medium sometimes give false-positive results due to instability of the blood used in the medium, we recommend the immunological techniques described here as reproducible methods for identification of the thermostable direct hemolysin by V. parahaemolyticus.

Full text

PDF
600

Images in this article

602Image
on p.602
602Image
on p.602

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chun D., Chung J. K., Tak R., Seol S. Y. Nature of the Kanagawa phenomenon of Vibrio parahaemolyticus. Infect Immun. 1975 Jul;12(1):81–87. doi: 10.1128/iai.12.1.81-87.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ELEK S. D. The recognition of toxicogenic bacterial strains in vitro. Br Med J. 1948 Mar 13;1(4549):493–496. doi: 10.1136/bmj.1.4549.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goshima K., Honda T., Hirata M., Kikuchi K., Takeda Y. Stopping of the spontaneous beating of mouse and rat myocardial cells in vitro by a toxin from Vibrio parahaemolyticus. J Mol Cell Cardiol. 1977 Mar;9(3):191–213. doi: 10.1016/0022-2828(77)90029-3. [DOI] [PubMed] [Google Scholar]
  4. Honda T., Finkelstein R. A. Selection and characteristics of a Vibrio cholerae mutant lacking the A (ADP-ribosylating) portion of the cholera enterotoxin. Proc Natl Acad Sci U S A. 1979 Apr;76(4):2052–2056. doi: 10.1073/pnas.76.4.2052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Honda T., Goshima K., Takeda Y., Sugino Y., Miwatani T. Demonstration of the cardiotoxicity of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus. Infect Immun. 1976 Jan;13(1):163–171. doi: 10.1128/iai.13.1.163-171.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Honda T., Taga S., Takeda T., Hasibuan M. A., Takeda Y., Miwatani T. Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin. Infect Immun. 1976 Jan;13(1):133–139. doi: 10.1128/iai.13.1.133-139.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miyamoto Y., Kato T., Obara Y., Akiyama S., Takizawa K., Yamai S. In vitro hemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J Bacteriol. 1969 Nov;100(2):1147–1149. doi: 10.1128/jb.100.2.1147-1149.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Niikawa T., Obara Y., Yamai S., Miyamoto Y. Purification of a hemolysin from Vibrio parahaemolyticus. Jpn J Med Sci Biol. 1972 Jun;25(3):197–200. [PubMed] [Google Scholar]
  9. Sakazaki R., Tamura K., Kato T., Obara Y., Yamai S. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. 3. Enteropathogenicity. Jpn J Med Sci Biol. 1968 Oct;21(5):325–331. doi: 10.7883/yoken1952.21.325. [DOI] [PubMed] [Google Scholar]
  10. Sakurai J., Matsuzaki A., Miwatani T. Purification and characterization of thermostable direct hemolysin of Vibrio parahaemolyticus. Infect Immun. 1973 Nov;8(5):775–780. doi: 10.1128/iai.8.5.775-780.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sakurai J., Matsuzaki A., Takeda Y., Miwatani T. Existence of two distinct hemolysins in Vibrio parahaemolyticus. Infect Immun. 1974 May;9(5):777–780. doi: 10.1128/iai.9.5.777-780.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shinoda S., Honda T., Takeda Y., Miwatani T. Antigenic difference between polar montrichous and peritrichous flagella of Vibrio parahaemolyticus. J Bacteriol. 1974 Nov;120(2):923–928. doi: 10.1128/jb.120.2.923-928.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vasil M. L., Holmes R. K., Finkelstein R. A. Studies on toxinogenesis in Vibrio cholerae. II. An vitro test for enterotoxin production. Infect Immun. 1974 Jan;9(1):195–197. doi: 10.1128/iai.9.1.195-197.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yanagase Y., Inoue K., Ozaki M., Ochi T., Amano T. Hemolysins and related enzymes of Vibrio parahaemolyticus. I. Identification and partial purification of enzymes. Biken J. 1970 Jun;13(2):77–92. [PubMed] [Google Scholar]
  15. Zen-Yoji H., Hitokoto H., Morozumi S., Le Clair R. A. Purification and characterization o;f a hemolysin produced by Vibrio parahaemolyticus. J Infect Dis. 1971 Jun;123(6):665–667. doi: 10.1093/infdis/123.6.665. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES