Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Jun;11(6):625–630. doi: 10.1128/jcm.11.6.625-630.1980

Cellular fatty acid composition of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus.

S D Braunthal, S C Holt, A C Tanner, S S Socransky
PMCID: PMC273474  PMID: 7430333

Abstract

Strains of Actinobacillus actinomycetemcomitans isolated from deep pockets of patients with juvenile periodontitis were analyzed for their content of cellular fatty acids. Oral Haemophilus strains, morphologically and biochemically similar to Haemophilus aphrophilus, were also examined for their content of cellular fatty acids. The extractable lipids of the actinobacilli represented approximately 10% of the cell dry weight, with the bound lipids representing 2 to 5%. The major fatty acids consisted of myristic (C14:0) and palmitic (C16:0) acids and a C16:1 acid, possibly palmitoleic acid, accounting for 21, 35, and 31% of the total extractable fatty acids, respectively. Haemophilus strains had a similar cellular fatty acid content.

Full text

PDF
625

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alley C. C., Brooks J. B., Kellogg D. S., Jr Electron capture gas-liquid chromatographic-mass spectral identification of acids produced by Neisseria meningitidis in a defined medium. J Clin Microbiol. 1979 Jan;9(1):97–102. doi: 10.1128/jcm.9.1.97-102.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. Bovre K., Hytta R., Jantzen E., Froholm L. O. Gas chromatography of bacterial whole cell methanolysates. 3. Group relations of Neisseriae and Moraxellae. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(5):683–689. doi: 10.1111/j.1699-0463.1972.tb00194.x. [DOI] [PubMed] [Google Scholar]
  4. CARROLL K. K. Quantitative estimation of peak areas in gas-liquid chromatography. Nature. 1961 Jul 22;191:377–378. doi: 10.1038/191377a0. [DOI] [PubMed] [Google Scholar]
  5. Cullen J., Phillips M. C., Shipley G. G. The effects of temperature on the composition and physical properties of the lipids of Pseudomonas fluorescens. Biochem J. 1971 Dec;125(3):733–742. doi: 10.1042/bj1250733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ferber E., Schlecht S., Fromme I. Untersuchungen zur Fettsäure-Zusammensetzung von Lipiden bei Salmonella minnesota-S- und -R-Formen. Zentralbl Bakteriol Orig A. 1976 Nov;236(2-3):275–287. [PubMed] [Google Scholar]
  7. Finnerty W. R., Makula R. A., Feeley J. C. Cellular lipids of the Legionnaires' disease bacterium. Ann Intern Med. 1979 Apr;90(4):631–634. doi: 10.7326/0003-4819-90-4-631. [DOI] [PubMed] [Google Scholar]
  8. Goldfine H. Comparative aspects of bacterial lipids. Adv Microb Physiol. 1972;8:1–58. doi: 10.1016/s0065-2911(08)60187-3. [DOI] [PubMed] [Google Scholar]
  9. Hancock I. C., Humphreys G. O., Meadow P. M. Characterisation of the hydroxy acids of Pseudomonas aeruginosa 8602. Biochim Biophys Acta. 1970 Mar 10;202(2):389–391. doi: 10.1016/0005-2760(70)90204-3. [DOI] [PubMed] [Google Scholar]
  10. Holt S. C., Forcier G., Takacs B. J. Fatty acid composition of gliding bacteria: oral isolates of Capnocytophaga compared with Sporocytophaga. Infect Immun. 1979 Oct;26(1):298–304. doi: 10.1128/iai.26.1.298-304.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Irving J. T., Newman M. G., Socransky S. S., Heely J. D. Histological changes in experimental periodontal disease in rats mono-infected with a gram-negative organism. Arch Oral Biol. 1975 Mar;20(3):219–220. doi: 10.1016/0003-9969(75)90013-8. [DOI] [PubMed] [Google Scholar]
  12. Kaltenbach C. M., Moss C. W., Weaver R. E. Cultural and biochemical characteristics and fatty acid composition of Pseudomonas diminuta and Pseudomonas vesiculare. J Clin Microbiol. 1975 Apr;1(4):339–344. doi: 10.1128/jcm.1.4.339-344.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  14. Kilian M., Schiott C. R. Haemophili and related bacteria in the human oral cavity. Arch Oral Biol. 1975 Dec;20(12):791–796. doi: 10.1016/0003-9969(75)90055-2. [DOI] [PubMed] [Google Scholar]
  15. Kramzar G. R., Lynch D. L. A qualitative and quantitative study of the fatty acid composition of selected micro-organisms. Microbios. 1976;17(67):7–16. [PubMed] [Google Scholar]
  16. Lambert M. A., Hollis D. G., Moss C. W., Weaver R. E., Thomas M. L. Cellular fatty acids of nonpathogenic Neisseria. Can J Microbiol. 1971 Dec;17(12):1491–1502. doi: 10.1139/m71-239. [DOI] [PubMed] [Google Scholar]
  17. Lewis V. J., Weaver R. E., Hollis D. G. Fatty acid composition of Neisseria species as determined by gas chromatography. J Bacteriol. 1968 Jul;96(1):1–5. doi: 10.1128/jb.96.1.1-5.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lindner J. G., Marcelis J. H. Quantitative gas chromatography of Bacteroides species under different growth conditions. Antonie Van Leeuwenhoek. 1978;44(1):1–14. doi: 10.1007/BF00400072. [DOI] [PubMed] [Google Scholar]
  19. Moss C. W., Dees S. B. Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens. J Clin Microbiol. 1976 Dec;4(6):492–502. doi: 10.1128/jcm.4.6.492-502.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moss C. W., Dees S. B. Further studies of the cellular fatty acid composition of Legionnaires disease bacteria. J Clin Microbiol. 1979 May;9(5):648–649. doi: 10.1128/jcm.9.5.648-649.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Moss C. W., Dees S. B. Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids. J Chromatogr. 1975 Oct 29;112:594–604. doi: 10.1016/s0021-9673(00)99988-6. [DOI] [PubMed] [Google Scholar]
  22. Moss C. W., Kellogg D. S., Jr, Farshy D. C., Lambert M. A., Thayer J. D. Cellular fatty acids of pathogenic Neisseria. J Bacteriol. 1970 Oct;104(1):63–68. doi: 10.1128/jb.104.1.63-68.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moss C. W., Samuels S. B., Weaver R. E. Cellular fatty acid composition of selected Pseudomonas species. Appl Microbiol. 1972 Oct;24(4):596–598. doi: 10.1128/am.24.4.596-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. Cellular fatty acid composition of isolates from Legionnaires disease. J Clin Microbiol. 1977 Aug;6(2):140–143. doi: 10.1128/jcm.6.2.140-143.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Newman M. G., Socransky S. S. Predominant cultivable microbiota in periodontosis. J Periodontal Res. 1977 Mar;12(2):120–128. doi: 10.1111/j.1600-0765.1977.tb00114.x. [DOI] [PubMed] [Google Scholar]
  26. Newman M. G., Socransky S. S., Savitt E. D., Propas D. A., Crawford A. Studies of the microbiology of periodontosis. J Periodontol. 1976 Jul;47(7):373–379. doi: 10.1902/jop.1976.47.7.373. [DOI] [PubMed] [Google Scholar]
  27. Samuels S. B., Moss C. W., Weaver R. E. The fatty acids of Pseudomonas multivorans (Pseudomonas cepacia) and Pseudomonas kingii. J Gen Microbiol. 1973 Feb;74(2):275–279. doi: 10.1099/00221287-74-2-275. [DOI] [PubMed] [Google Scholar]
  28. Sims W. Oral haemophili. J Med Microbiol. 1970 Nov;3(4):615–625. doi: 10.1099/00222615-3-4-615. [DOI] [PubMed] [Google Scholar]
  29. Slots J. The predominant cultivable organisms in juvenile periodontitis. Scand J Dent Res. 1976 Jan;84(1):1–10. doi: 10.1111/j.1600-0722.1976.tb00454.x. [DOI] [PubMed] [Google Scholar]
  30. Sud I. J., Feingold D. S. Phospholipids and fatty acids of Neisseria gonorrhoeae. J Bacteriol. 1975 Nov;124(2):713–717. doi: 10.1128/jb.124.2.713-717.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. VACZI L., RETHY A., REDAI I. FATTY ACIDS IN ENTERIC BACTERIA. Acta Microbiol Acad Sci Hung. 1964;11:375–382. [PubMed] [Google Scholar]
  32. WELLS M. A., DITTMER J. C. THE USE OF SEPHADEX FOR THE REMOVAL OF NONLIPID CONTAMINANTS FROM LIPID EXTRACTS. Biochemistry. 1963 Nov-Dec;2:1259–1263. doi: 10.1021/bi00906a015. [DOI] [PubMed] [Google Scholar]
  33. van Palenstein Helderman W. H. Total viable count and differential count of vibrio (campylobacter) sputorum, fusobacterium nucleatum, selenomonas sputigena, bacteroides ochraceus and veillonella in the inflamed and non inflamed human gingival crevice. J Periodontal Res. 1975 Nov;10(5):294–305. doi: 10.1111/j.1600-0765.1975.tb00037.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES