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. 1980 Jul;12(1):1–6. doi: 10.1128/jcm.12.1.1-6.1980

Micro-indirect hemagglutination test for detection of antibodies to the Ibc protein of group B Streptococcus.

C P Thangavelu, G Koshi
PMCID: PMC273507  PMID: 6999011

Abstract

A micro-indirect hemagglutination test was developed for detecting antibody against the Ibc protein of group B Streptococcus. Formalin-preserved, tanned sheep erythrocytes were sensitized with a partially purified preparation of Ibc protein from a type Ic strain of group B streptococci. A total of 76% of 103 sera from pregnant and nonpregnant women had demonstrable antibody against this protein, with titers ranging from 10 to 320. Examination of five pairs of mother and cord sera revealed passive transfer of these antibiodies from mother to infant. This testing on a limited number of sera also revealed that elevated antibody titers against Ibc protein were more common among carriers of group B streptococci, especially those harboring strains with Ibc protein antigen, than among noncarriers. The technique described was found to be simple, specific, sensitive, and reproducible and may be of value in assessing the immune status of pregnant women as well as for epidemiological purposes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anthony B. F. Immunity to the group B streptococci: interaction of serum and macrophages with types Ia, Ib, and Ic. J Exp Med. 1976 May 1;143(5):1186–1198. doi: 10.1084/jem.143.5.1186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker C. J., Clark D. J., Barrett F. F. Selective broth medium for isolation of group B streptococci. Appl Microbiol. 1973 Dec;26(6):884–885. doi: 10.1128/am.26.6.884-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker C. J., Kasper D. L. Correlation of maternal antibody deficiency with susceptibility to neonatal group B streptococcal infection. N Engl J Med. 1976 Apr 1;294(14):753–756. doi: 10.1056/NEJM197604012941404. [DOI] [PubMed] [Google Scholar]
  4. Baltimore R. S., Kasper D. L., Baker C. J., Goroff D. K. Antigenic specificity of opsonophagocytic antibodies in rabbit anti-sera to group B streptococci. J Immunol. 1977 Feb;118(2):673–678. [PubMed] [Google Scholar]
  5. Baltimore R. S., Kasper D. L., Vecchitto J. Mouse protection test for group B Streptococcus type III. J Infect Dis. 1979 Jul;140(1):81–88. doi: 10.1093/infdis/140.1.81. [DOI] [PubMed] [Google Scholar]
  6. Ferrieri P., Cleary P. P., Seeds A. E. Epidemiology of group-B streptococcal carriage in pregnant women and newborn infants. J Med Microbiol. 1977 Feb;10(1):103–114. doi: 10.1099/00222615-10-1-103. [DOI] [PubMed] [Google Scholar]
  7. Hall R. T., Barnes W., Krishnan L., Harris D. J., Rhodes P. G., Fayez J., Miller G. L. Antibiotic treatment of parturient women colonized with group B streptococci. Am J Obstet Gynecol. 1976 Mar 15;124(6):630–634. doi: 10.1016/0002-9378(76)90065-x. [DOI] [PubMed] [Google Scholar]
  8. Hemming V. G., Hall R. T., Rhodes P. G., Shigeoka A. O., Hill H. R. Assessment of group B streptococcal opsonins in human and rabbit serum by neutrophil chemiluminescence. J Clin Invest. 1976 Dec;58(6):1379–1387. doi: 10.1172/JCI108593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hill H. R., Sauls H. S., Dettloff J. L., Quie P. G. Impaired leukotactic responsiveness in patients with juvenile diabetes mellitus. Clin Immunol Immunopathol. 1974 Apr;2(3):395–403. doi: 10.1016/0090-1229(74)90057-9. [DOI] [PubMed] [Google Scholar]
  10. Kasper D. L., Baker C. J. Electron microscopic definition of surface antigens of group B Streptococcus. J Infect Dis. 1979 Feb;139(2):147–151. doi: 10.1093/infdis/139.2.147. [DOI] [PubMed] [Google Scholar]
  11. Klesius P. H., Zimmerman R. A., Mathews J. H., Krushak D. H. Cellular and humoral immune response to group B streptococci. J Pediatr. 1973 Dec;83(6):926–932. doi: 10.1016/s0022-3476(73)80525-6. [DOI] [PubMed] [Google Scholar]
  12. LANCEFIELD R. C. Differentiation of group A streptococci with a common R antigen into three serological types, with special reference to the bactericidal test. J Exp Med. 1957 Oct 1;106(4):525–544. doi: 10.1084/jem.106.4.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lancefield R. C., Freimer E. H. Type-specific polysaccharide antigens of group B streptococci. J Hyg (Lond) 1966 Jun;64(2):191–203. doi: 10.1017/s0022172400040456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lancefield R. C., McCarty M., Everly W. N. Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens. J Exp Med. 1975 Jul 1;142(1):165–179. doi: 10.1084/jem.142.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PATTISON I. H., MATTHEWS P. R., HOWELL D. G. The type classification of group-B streptococci, with special reference to bovine strains apparently lacking in type polysaccharide. J Pathol Bacteriol. 1955 Jan-Apr;69(1-2):51–60. [PubMed] [Google Scholar]
  17. Perch B., Kjems E., Henrichsen New serotypes of group B streptococci isolated from human sources. J Clin Microbiol. 1979 Jul;10(1):109–110. doi: 10.1128/jcm.10.1.109-110.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schauf V., Hlaing V. Group B streptococcal colonization in pregnancy. Obstet Gynecol. 1976 Jun;47(6):719–721. [PubMed] [Google Scholar]
  19. Stewardson-Krieger P. B., Albrandt K., Nevin T., Kretschmer R. R., Gotoff S. P. Perinatal immunity to group B beta-hemolytic streptococcus type Ia. J Infect Dis. 1977 Nov;136(5):649–654. doi: 10.1093/infdis/136.5.649. [DOI] [PubMed] [Google Scholar]
  20. Stewardson-Krieger P., Albrandt K., Kretschmer R. R., Gotoff S. P. Group B streptococcal long-chain reaction. Infect Immun. 1977 Dec;18(3):666–672. doi: 10.1128/iai.18.3.666-672.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Summary of the workshop on perinatal infections due to group B Streptococcus. J Infect Dis. 1977 Jul;136(1):137–152. doi: 10.1093/infdis/136.1.137. [DOI] [PubMed] [Google Scholar]
  22. Wilkinson H. W. Analysis of group B streptococcal types associated with disease in human infants and adults. J Clin Microbiol. 1978 Feb;7(2):176–179. doi: 10.1128/jcm.7.2.176-179.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wilkinson H. W. Detection of group B streptococcal antibodies in human sera by radioimmunoassay: concentrations of type-specific antibodies in sera of adults and infants infected with group B streptococci. J Clin Microbiol. 1978 Feb;7(2):194–201. doi: 10.1128/jcm.7.2.194-201.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wilkinson H. W., Eagon R. G. Type-specific antigens of group B type Ic streptococci. Infect Immun. 1971 Nov;4(5):596–604. doi: 10.1128/iai.4.5.596-604.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilkinson H. W., Facklam R. R., Wortham E. C. Distribution by serological type of group B streptococci isolated from a variety of clinical material over a five-year period (with special reference to neonatal sepsis and meningitis). Infect Immun. 1973 Aug;8(2):228–235. doi: 10.1128/iai.8.2.228-235.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilkinson H. W. Group B streptococcal infection in humans. Annu Rev Microbiol. 1978;32:41–57. doi: 10.1146/annurev.mi.32.100178.000353. [DOI] [PubMed] [Google Scholar]
  27. Zimmerman R. A., Mathews J., Wilson E. Microtiter indirect hemagglutination procedure for identification of streptococcal M-protein antibodies. Appl Microbiol. 1968 Nov;16(11):1640–1645. doi: 10.1128/am.16.11.1640-1645.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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