Abstract
A broth medium that supports growth of Neisseria gonorrhoeae to high densities with minimal lysis is described. With most gonococcal strains, inocula of 2 x 10(6) colony-forming units per ml yielded greater than 109 colony-forming units per ml after 8 h of incubation. Scale-up cultures produced 5 to 12 g (wet weight) of cells per liter.
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- Brookes R., Hedén C. G. Dense cultures of Neisseria gonorrhoeae in liquid medium. Appl Microbiol. 1967 Mar;15(2):219–223. doi: 10.1128/am.15.2.219-223.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brookes R., Sikyta B. Influence of pH on the growth characteristics of Neisseria gonorrhoeae in continuous culture. Appl Microbiol. 1967 Mar;15(2):224–227. doi: 10.1128/am.15.2.224-227.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chan K., Wiseman G. M., Caird J. D. Cultivation of type 1 N. Gonorrhoeae in liquid media. Br J Vener Dis. 1975 Dec;51(6):382–386. doi: 10.1136/sti.51.6.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frantz I. D. Growth Requirements of the Meningococcus. J Bacteriol. 1942 Jun;43(6):757–761. doi: 10.1128/jb.43.6.757-761.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERHARDT P., HEDEN C. G. Concentrated culture of gonococci in clear liquid medium. Proc Soc Exp Biol Med. 1960 Oct;105:49–51. doi: 10.3181/00379727-105-26005. [DOI] [PubMed] [Google Scholar]
- Hafiz S., McEntegart M. G., Jephcott A. E. Reversion of Kellogg's colonial types of Neisseria gonorrhoeae in liquid medium. J Med Microbiol. 1977 Aug;10(3):377–380. doi: 10.1099/00222615-10-3-377. [DOI] [PubMed] [Google Scholar]
- Hafiz S., McEntegart M. G. Prolonged survival of Neisseria gonorrhoeae in a new liquid medium. Br J Vener Dis. 1976 Dec;52(6):381–383. doi: 10.1136/sti.52.6.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hart E. J., Goldberg I. D. Conditions required for the attainment of colony-type stability of Neisseria gonorrhoeae in liquid culture. J Clin Microbiol. 1975 Nov;2(5):387–390. doi: 10.1128/jcm.2.5.387-390.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jephcott A. E. Preliminary study of colony type stability of Neisseria gonorrhoeae in liquid culture. Br J Vener Dis. 1972 Oct;48(5):369–375. doi: 10.1136/sti.48.5.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones R. T., Talley R. S. Simplified complete medium for the growth of Neisseria gonorrhoeae. J Clin Microbiol. 1977 Jan;5(1):9–14. doi: 10.1128/jcm.5.1.9-14.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayer L. W., Schoolnik G. K., Falkow S. Genetic studies on Neisseria gonorrhoeae from disseminated gonococcal infections. Infect Immun. 1977 Oct;18(1):165–172. doi: 10.1128/iai.18.1.165-172.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAUBER H., GARSON W. Preparation and some properties of Neisseria gonorrhoeae endotoxin. Proc Soc Exp Biol Med. 1957 Aug-Sep;95(4):669–672. doi: 10.3181/00379727-95-23323. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
- Wong T. P., Shockley R. K., Johnston K. H. WSJM, a simple chemically defined medium for growth of Neisseria gonorrhoeae. J Clin Microbiol. 1980 Apr;11(4):363–369. doi: 10.1128/jcm.11.4.363-369.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]