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. 1980 Jul;12(1):101–104. doi: 10.1128/jcm.12.1.101-104.1980

Detection of rotavirus by serological trapping on antibody-coated electron microscope grids.

A Nicolaieff, G Obert, M H van Regenmortel
PMCID: PMC273529  PMID: 6252237

Abstract

A serological trapping technique for detecting rotaviruses is described which involves coating electron microscope grids with protein A and specific rotavirus antiserum. The presence of a layer of antibodies on the grid increases the number of rotavirus particles that can be visualized. Thirty-five crude fecal extracts from infants suffering from diarrhea were examined by the serological trapping technique and by standard electron-microscopy. When the specimens were deposited on antibody-coated girds, 71% of them were found to contain virus particles, compared with 20% on standard uncoated grids. The method is simple and rapid and does away with the need to concentrate the specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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