Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Aug;12(2):138–139. doi: 10.1128/jcm.12.2.138-139.1980

Prothrombin activation by a metalloprotease from Staphylococcus aureus.

Z Wegrzynowicz, P B Heczko, G R Drapeau, J Jeljaszewicz, G Pulverer
PMCID: PMC273542  PMID: 6785302

Abstract

Formation of thrombin during incubation of purified bovine prothrombin with purified staphylococcal metalloprotease has been investigated. Thrombin activity was estimated by examination of clotting time and by digestion of a synthetic substrate, Chromozym TH. The metalloprotease caused direct activation of prothrombin which was inhibited by the addition of ethylenediaminetetraacetic acid. Metalloprotease produced by some strains of Staphylococcus aureus may simulate staphylocoagulase activity.

Full text

PDF
138

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arvidson S. Studies on extracellular proteolytic enzymes from Staphylococcus aureus. II. Isolation and characterization of an EDTA-sensitive protease. Biochim Biophys Acta. 1973 Mar 15;302(1):149–157. doi: 10.1016/0005-2744(73)90017-x. [DOI] [PubMed] [Google Scholar]
  2. Björklind A., Arvidson S. Occurrence of an extracellular serineproteinase among Staphylococcus aureus strains. Acta Pathol Microbiol Scand B. 1977 Aug;85(4):277–280. doi: 10.1111/j.1699-0463.1977.tb01974.x. [DOI] [PubMed] [Google Scholar]
  3. Drapeau G. R. Role of metalloprotease in activation of the precursor of staphylococcal protease. J Bacteriol. 1978 Nov;136(2):607–613. doi: 10.1128/jb.136.2.607-613.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Houmard J., Drapeau G. R. Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3506–3509. doi: 10.1073/pnas.69.12.3506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KEKWICK R. A., MACKAY M. E., NANCE M. H., RECORD B. R. The purification of human fibrinogen. Biochem J. 1955 Aug;60(4):671–683. doi: 10.1042/bj0600671b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kornalik F., Blombäck B. Prothrombin activation induced by Ecarin - a prothrombin converting enzyme from Echis carinatus venom. Thromb Res. 1975 Jan;6(1):57–63. doi: 10.1016/0049-3848(75)90150-4. [DOI] [PubMed] [Google Scholar]
  7. Malhotra O. P., Carter J. R. Modified method for the preparation of purified bovine prothrombin of high specific activity. Thromb Diath Haemorrh. 1968 Mar 31;19(1):178–185. [PubMed] [Google Scholar]
  8. Parisi J. T. Staphylococcus epidermidis, and emerging pathogen. Mo Med. 1973 Apr;70(4):243–244. [PubMed] [Google Scholar]
  9. Switalski L. M., Schwam O., Smyth C. J., Wadström T. Peptocoagulase: clotting factor produced by bovine strains of Peptococcus indolicus. J Clin Microbiol. 1978 Apr;7(4):361–367. doi: 10.1128/jcm.7.4.361-367.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wegrzynowicz Z., Heczko P. B., Jeljaszewicz J., Neugebauer M., Pulverer G. Pseudocoagulase activity of staphylococci. J Clin Microbiol. 1979 Jan;9(1):15–19. doi: 10.1128/jcm.9.1.15-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wegrzynowicz Z., Ko H. J., Pulverer G., Jeljaszewicz J. The nature of clotting and fibrinolytic activities of Bacteroids melaninogenicus. Zentralbl Bakteriol Orig A. 1978 Jan;240(1):106–111. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES