Abstract
We have developed a simple and sensitive chemiluminescence-linked immunoassay (CLIA) for determining mumps virus antibodies. Luminol molecules were used as markers, and polystyrene balls were used as antigen carriers. The CLIA was compared with an enzyme-linked fluorescence assay and a hemagglutination inhibition test on a total of 40 serum specimens obtained from 29 donors with natural infection or vaccination. There was good correlation between the three methods, and the sensitivity of the CLIA was about 10 times higher than that of the hemagglutination inhibition test, although it was slightly inferior to that of the enzyme-linked fluorescence assay. Moreover, the time course of light emission from the labeled antibody was rapid, and therefore in the CLIA the quantitation of the marker takes only a short time.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Daugharty H., Warfield D. T., Hemingway W. D., Casey H. L. Mumps class-specific immunoglobulins in radioimmunoassay and conventional serology. Infect Immun. 1973 Mar;7(3):380–385. doi: 10.1128/iai.7.3.380-385.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- East J. L., Kingsbury D. W. Mumps virus replication in chick embryo lung cells: properties of ribonucleic acids in virions and infected cells. J Virol. 1971 Aug;8(2):161–173. doi: 10.1128/jvi.8.2.161-173.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glick D. The future of clinical microchemical analysis. Clin Chem. 1978 Feb;24(2):189–192. [PubMed] [Google Scholar]
- Iwasa S., Ueno H., Miya T., Wakismasu M., Kondo K., Ohneda A. Enzyme immunoassay of pancreatic glucagon at the picogram level using beta-D-galactosidase as a label. J Biochem. 1979 Oct;86(4):943–949. doi: 10.1093/oxfordjournals.jbchem.a132626. [DOI] [PubMed] [Google Scholar]
- Kalimo K. O., Ziola B. R., Viljanen M. K., Granfors K., Toivanen P. Solid-phase radioimmunoassay of herpes simplex virus IgG and IgM antibodies. J Immunol Methods. 1977;14(2):183–195. doi: 10.1016/0022-1759(77)90008-4. [DOI] [PubMed] [Google Scholar]
- Kitagawa T., Aikawa T. Enzyme coupled immunoassay of insulin using a novel coupling reagent. J Biochem. 1976 Jan;79(1):233–236. doi: 10.1093/oxfordjournals.jbchem.a131053. [DOI] [PubMed] [Google Scholar]
- Kohen F., Pazzagli M., Kim J. B., Lindner H. R., Boguslaski R. C. An assay procedure for plasma progesterone based on antibody-enhanced chemiluminescence. FEBS Lett. 1979 Aug 1;104(1):201–205. doi: 10.1016/0014-5793(79)81115-1. [DOI] [PubMed] [Google Scholar]
- LEVY H. B., SOBER H. A. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960 Jan;103:250–252. doi: 10.3181/00379727-103-25476. [DOI] [PubMed] [Google Scholar]
- Leinikki P. O., Shekarchi I., Tzan N., Madden D. L., Sever J. L. Evaluation of enzyme-linked immunosorbent assay (ELISA) for mumps virus antibodies. Proc Soc Exp Biol Med. 1979 Mar;160(3):363–367. doi: 10.3181/00379727-160-40451. [DOI] [PubMed] [Google Scholar]
- Pratt J. J., Woldring M. G., Villerius L. Chemiluminescence-linked immunoassay. J Immunol Methods. 1978;21(1-2):179–184. doi: 10.1016/0022-1759(78)90234-x. [DOI] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- Simpson J. S., Campbell A. K., Ryall M. E., Woodhead J. S. A stable chemiluminescent-labelled antibody for immunological assays. Nature. 1979 Jun 14;279(5714):646–647. doi: 10.1038/279646a0. [DOI] [PubMed] [Google Scholar]
- WALLENFELS K., ZARNITZ M. L., LAULE G., BENDER H., KESER M. [Studies on lactic acid splitting enzyme. III. Purification, crystallization and features of beta-galctosidase of Escherichia coli ML 309]. Biochem Z. 1959;331:459–485. [PubMed] [Google Scholar]