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. 1980 Aug;12(2):156–160. doi: 10.1128/jcm.12.2.156-160.1980

Cultural and biochemical characteristics of clinical isolates of unusual colistin-resistant pseudomonads.

T R Oberhofer
PMCID: PMC273546  PMID: 7229004

Abstract

Biochemical characteristics and antibiotic susceptibilities of 12 strains of colistin-resistant pseudomonads isolated from clinical specimens are reported. The isolates were short, oxidase-positive, nonfluorescing, gram-negative rods that failed to grow on salmonella-shigella or cetrimide agars, to decarboxylate amino acids, and to reduce nitrates. Most strains peptonized litmus milk and grew at 42 degrees C. Glucose, lactose, maltose, xylose, and fructose were slowly oxidized, whereas sucrose was not. Two homogeneous species were found and tentatively listed as Pseudomonas sp. 1 and Pseudomonas sp. 2, and these were differentiated by gelatin and starch hydrolysis, oxidation of mannitol, and alkalinization of allantoin. The two species were shown to differ from the Center for Disease Control Va group biotype CDC Va-1 in both biochemical characteristics and susceptibility to the aminoglycosides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fass R. J., Barnishan J. Letter: Acute meningitis due to a Pseudomonas-like Group Va-1 bacillus. Ann Intern Med. 1976 Jan;84(1):51–52. doi: 10.7326/0003-4819-84-1-51. [DOI] [PubMed] [Google Scholar]
  2. Garcia J. L., Pichinoty F., Mandel M., Greenway B. A new denitrifying saprophyte related to Pseudomonas pickettii. Ann Microbiol (Paris) 1977 Feb-Mar;128(2):229–237. [PubMed] [Google Scholar]
  3. Graber C. D., Jervey L. P., Ostrander W. E., Salley L. H., Weaver R. E. Endocarditis due to a lanthanic, unclassified Gram-negative bacterium (group IV d). Am J Clin Pathol. 1968 Feb;49(2):220–223. doi: 10.1093/ajcp/49.2.220. [DOI] [PubMed] [Google Scholar]
  4. Knuth B. D., Owen M. R., Latorraca R. Occurrence of an unclassified organism, group IVd. Am J Med Technol. 1969 Apr;35(4):227–232. [PubMed] [Google Scholar]
  5. Moffet H. L., Williams T. Bacteria recovered from distilled water and inhalation therapy equipment. Am J Dis Child. 1967 Jul;114(1):7–12. doi: 10.1001/archpedi.1967.02090220013002. [DOI] [PubMed] [Google Scholar]
  6. Oberhofer T. R. Growth of nonfermentative bacteria at 42 degrees C. J Clin Microbiol. 1979 Dec;10(6):800–804. doi: 10.1128/jcm.10.6.800-804.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oberhofer T. R., Rowen J. W., Cunningham G. F. Characterization and identification of gram-negative, nonfermentative bacteria. J Clin Microbiol. 1977 Feb;5(2):208–220. doi: 10.1128/jcm.5.2.208-220.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Otto L. A., Deboo B. S., Capers E. L., Pickett M. J. Pseudomonas vesicularis from cervical specimens. J Clin Microbiol. 1978 Apr;7(4):341–345. doi: 10.1128/jcm.7.4.341-345.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Riley P. S., Weaver R. E. Recognition of Pseudomonas pickettii in the clinical laboratory: biochemical characterization of 62 strains. J Clin Microbiol. 1975 Jan;1(1):61–64. doi: 10.1128/jcm.1.1.61-64.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Washington J. A., 2nd Antimicrobial susceptibility of enterobacteriaceae and nonfermenting gram-negative bacilli. Mayo Clin Proc. 1969 Nov;44(11):811–824. [PubMed] [Google Scholar]
  11. von Graevenitz A. Clinical microbiology of unusual Pseudomonas species. Prog Clin Pathol. 1973;5:185–218. [PubMed] [Google Scholar]

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