Abstract
Sterile stainless-steel electrodes implanted in blood culture bottles and monitored electronically were used to detect growth of microorganisms. Each blood culture bottle contained 100 ml of medium and was inoculated with 10 ml of blood seeded with either 300 or 50 colony-forming units of one of several bacterial or yeast species that are commonly isolated from clinical blood cultures. Growth was indicated by a voltage change of at least 0.1 mV/min with an increasing slope over at least three consecutive 15-min intervals. This method was compared to the conventional visual method for detecting microbial growth in broth. Growth detection by both techniques was confirmed by subculture to solid media. Of the 163 cultures seeded with the high inoculum (300 colony-forming units) and confirmed as being positive, 148 (90.8%) were positive by the electronic detection system (EDS). At the lower inoculum (50 colony-forming units), 47 of 53 (88.7%) positive cultures were detected by EDS. Twelve of the 21 false-negatives by the EDS were cultures seeded with Cryptococcus neoformans. Excluding C. neoformans, the rate of detection of growth was 96.0%. Microbial growth was detected an average of 18 h earlier by EDS than by the conventional system in 176 (90.2%) of the cultures. Also examined were 156 patient blood cultures: 13 were positive both by EDS and by conventional methods.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DeBlanc H. J., Jr, DeLand F., Wagner H. N., Jr Automated radiometric detection of bacteria in 2,967 blood cultures. Appl Microbiol. 1971 Nov;22(5):846–849. doi: 10.1128/am.22.5.846-849.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeLand F. H., Wagner H. N., Jr Early detection of bacterial growth, with carbon-14-labeled glucose. Radiology. 1969 Jan;92(1):154–155. doi: 10.1148/92.1.154. [DOI] [PubMed] [Google Scholar]
- DeLand F., Wagner H. N., Jr Automated radiometric detection of bacterial growth in blood cultures. J Lab Clin Med. 1970 Mar;75(3):529–534. [PubMed] [Google Scholar]
- Hardy D., Kraeger S. J., Dufour S. W., Cady P. Rapid detection of microbial contamination in frozen vegetables by automated impedance measurements. Appl Environ Microbiol. 1977 Jul;34(1):14–17. doi: 10.1128/aem.34.1.14-17.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kagan R. L., Schuette W. H., Zierdt C. H., MacLowry J. D. Rapid automated disgnosis of bacteremia by impedance detection. J Clin Microbiol. 1977 Jan;5(1):51–57. doi: 10.1128/jcm.5.1.51-57.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lamb V. A., Dalton H. P., Wilkins J. R. Electrochemical method for the early detection of urinary-tract infections. Am J Clin Pathol. 1976 Jul;66(1):91–95. doi: 10.1093/ajcp/66.1.91. [DOI] [PubMed] [Google Scholar]
- Specter S., Throm R., Strauss R., Friedman H. Rapid detection of bacterial growth in blood samples by a continuous-monitoring electrical impedance apparatus. J Clin Microbiol. 1977 Nov;6(5):489–493. doi: 10.1128/jcm.6.5.489-493.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkins J. R., Stoner G. E., Boykin E. H. Microbial detection method based on sensing molecular hydrogen. Appl Microbiol. 1974 May;27(5):949–952. doi: 10.1128/am.27.5.949-952.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkins J. R. Use of platinum electrodes for the electrochemical detection of bacteria. Appl Environ Microbiol. 1978 Nov;36(5):683–687. doi: 10.1128/aem.36.5.683-687.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]