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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Aug;12(2):226–234. doi: 10.1128/jcm.12.2.226-234.1980

Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid:deoxyribonucleic acid hybridization.

A R Brautigam, D D Richman, M N Oxman
PMCID: PMC273559  PMID: 6262368

Abstract

A method for typing clinical isolates of herpes simplex virus was developed. It utilizes hybridization between unlabeled deoxyribonucleic acid from infected cultures and tritium-labeled virus deoxyribonucleic acid, and it can be completed within a day using a single roller-tube culture of the clinical isolated. The data obtained are inherently quantitative, and the method yields unequivocal identification and typing. Thirty-nine coded clinical isolates were all correctly typed by this method.

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Selected References

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