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. 1980 Sep;12(3):314–319. doi: 10.1128/jcm.12.3.314-319.1980

Measurement of immunoglobulin A, G, and M class rotavirus antibodies in serum and mucosal secretions.

B McLean, S Sonza, I H Holmes
PMCID: PMC273582  PMID: 6260831

Abstract

A solid-phase, enzyme-linked immunospecific assay for measurement of different immunoglobulin classes of human rotavirus antibodies is described. The antigen, which was adsorbed directly to polyvinyl microtiter plates, consisted of a clarified cell culture stock of the simian rotavirus SA 11. The assay was sensitive and reproducible and could readily be calibrated to determine concentrations of each class of antibody. The assay was applied to measurements of rotavirus antibodies in serum, colostrum, milk, and fecal samples. It particularly facilitates investigations of the role of immunoglobulin A antibodies in immunity to rotavirus infections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chanock R. M., Wyatt R. G., Kapikian A. Z. Immunization of infants and young children against rotaviral gastroenteritis--prospects and problems. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 2):570–572. [PubMed] [Google Scholar]
  2. Cukor G., Blacklow N. R., Capozza F. E., Panjvani Z. F., Bednarek F. Persistence of antibodies to rotavirus in human milk. J Clin Microbiol. 1979 Jan;9(1):93–96. doi: 10.1128/jcm.9.1.93-96.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
  4. Flewett T. H., Woode G. N. The rotaviruses. Arch Virol. 1978;57(1):1–23. doi: 10.1007/BF01315633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ghose L. H., Schnagl R. D., Holmes I. H. Comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey. J Clin Microbiol. 1978 Sep;8(3):268–276. doi: 10.1128/jcm.8.3.268-276.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herrmann J. E., Collins M. F. Quantitation of immunoglobulin adsorption to plastics. J Immunol Methods. 1976;10(4):363–366. doi: 10.1016/0022-1759(76)90030-2. [DOI] [PubMed] [Google Scholar]
  7. Holmes I. H., Rodger S. M., Schnagl R. D., Ruck B. J., Gust I. D., Bishop R. F., Barnes G. L. Is lactase the receptor and uncoating enzyme for infantile enteritis (rota) viruses? Lancet. 1976 Jun 26;1(7974):1387–1388. doi: 10.1016/s0140-6736(76)93032-4. [DOI] [PubMed] [Google Scholar]
  8. Holmes I. H. Viral gastroenteritis. Prog Med Virol. 1979;25:1–36. [PubMed] [Google Scholar]
  9. Kim H. W., Brandt C. D., Kapikian A. Z., Wyatt R. G., Arrobio J. O., Rodriguez W. J., Chanock R. M., Parrott R. H. Human reovirus-like agent infection. Occurrence in adult contacts of pediatric patients with gastroenteritis. JAMA. 1977 Aug 1;238(5):404–407. doi: 10.1001/jama.238.5.404. [DOI] [PubMed] [Google Scholar]
  10. McNulty M. S. Rotaviruses. J Gen Virol. 1978 Jul;40(1):1–18. doi: 10.1099/0022-1317-40-1-1. [DOI] [PubMed] [Google Scholar]
  11. Morilla A., Klemm R. C., Sprino P., Ristic M. Neutralization of a transmissible gastroenteritis virus of swine by colostral antibodies elicited by intestine and cell culture-propagated virus. Am J Vet Res. 1976 Sep;37(9):1011–1016. [PubMed] [Google Scholar]
  12. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  13. Schnagl R. D., Holmes I. H., Mackay-Scollay E. M. A survey of rotavirus associated with gastroenteritis in Aboriginal children in Western Australia. Med J Aust. 1978 Mar 25;1(6):304–307. doi: 10.5694/j.1326-5377.1978.tb107863.x. [DOI] [PubMed] [Google Scholar]
  14. Shearman D. J., Parkin D. M., McClelland D. B. The demonstration and function of antibodies in the gastrointestinal tract. Gut. 1972 Jun;13(6):483–499. doi: 10.1136/gut.13.6.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stiehm E. R., Fudenberg H. H. Serum levels of immune globulins in health and disease: a survey. Pediatrics. 1966 May;37(5):715–727. [PubMed] [Google Scholar]
  16. Svennerholm A., Lange S., Holmgren J. Correlation between intestinal synthesis of specific immunoglobulin A and protection against experimental cholera in mice. Infect Immun. 1978 Jul;21(1):1–6. doi: 10.1128/iai.21.1.1-6.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. The enzyme-linked immunosorbent assay (ELISA). Bull World Health Organ. 1976;54(2):129–139. [PMC free article] [PubMed] [Google Scholar]
  18. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol. 1977;53(4):287–294. doi: 10.1007/BF01315627. [DOI] [PubMed] [Google Scholar]
  19. Watanabe H., Holmes I. H. Filter paper solid-phase radioimmunoassay for human rotavirus surface immunoglobulins. J Clin Microbiol. 1977 Oct;6(4):319–324. doi: 10.1128/jcm.6.4.319-324.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yolken R. H., Wyatt R. G., Kim H. W., Kapikian A. Z., Chanock R. M. Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay. Infect Immun. 1978 Feb;19(2):540–546. doi: 10.1128/iai.19.2.540-546.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yolken R. H., Wyatt R. G., Mata L., Urrutia J. J., Garciá B., Chanock R. M., Kapikian A. Z. Secretory antibody directed against rotavirus in human milk--measurement by means of enzyme-linked immunosorbent assay. J Pediatr. 1978 Dec;93(6):916–921. doi: 10.1016/s0022-3476(78)81211-6. [DOI] [PubMed] [Google Scholar]

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