Abstract
Staining of clinical respiratory specimens obtained by nasopharyngeal and throat swabs with direct fluorescein isothiocyanate-conjugated antisera to para-influenza virus types 1, 2, and 3, influenza A virus, respiratory syncytial virus, adenovirus, mumps virus, and measles virus was compared with isolation procedures in routine tissue culture systems. Direct staining of clinical specimens with the commercially available conjugated offered a rapid means of diagnosing respiratory infections on a routine clinical basis when used in conjunction with isolation in tissue culture systems. Of 292 patients who were culture positive for these viruses, 259 were diagnosed by detection of the viral antigen in clinical specimens by direct immunofluorescence. No specimens that subsequently yielded a different respiratory virus by culture were positive by the direct immunofluorescence method. Use of selected antisera based on clinical history of the patient reduced the cost of rapid viral diagnosis.
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- Bjorvatn B., Wolontis S. The use of immunofluorescence in the laboratory diagnosis of influenza type B. Scand J Infect Dis. 1972;4(3):177–182. doi: 10.3109/inf.1972.4.issue-3.02. [DOI] [PubMed] [Google Scholar]
- D'Alessio D., Williams S., Dick E. C. Rapid detection and identification of respiratory viruses by direct immunofluorescence. Appl Microbiol. 1970 Aug;20(2):233–239. doi: 10.1128/am.20.2.233-239.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daisy J. A., Lief F. S., Friedman H. M. Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults. J Clin Microbiol. 1979 Jun;9(6):688–692. doi: 10.1128/jcm.9.6.688-692.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fulton R. E., Middleton P. J. Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children. Infect Immun. 1974 Jul;10(1):92–101. doi: 10.1128/iai.10.1.92-101.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fulton R. E., Middleton P. J. Immunofluorescence in diagnosis of measles infections in children. J Pediatr. 1975 Jan;86(1):17–22. doi: 10.1016/s0022-3476(75)80698-6. [DOI] [PubMed] [Google Scholar]
- Gardner P. S., McQuillin J., McGuckin R., Ditchburn R. K. Observations on clinical and immunofluorescent diagnosis of parainfluenza virus infections. Br Med J. 1971 Apr 3;2(5752):7–12. doi: 10.1136/bmj.2.5752.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardner P. S., McQuillin J., McGuckin R. The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond) 1970 Dec;68(4):575–580. doi: 10.1017/s0022172400042509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knight V., Brasier F., Greenberg S. B., Jones D. B. Immunofluorescent diagnosis of acute viral infection. South Med J. 1975 Jun;68(6):764–766. doi: 10.1097/00007611-197506000-00027. [DOI] [PubMed] [Google Scholar]
- McQuillin J., Gardner P. S. Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques. Br Med J. 1968 Mar 9;1(5592):602–605. doi: 10.1136/bmj.1.5592.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartz H. S., Vastine D. W., Yamashiroya H., West C. E. Immunofluorescent detection of adenovirus antigen in epidemic keratoconjunctivitis. Invest Ophthalmol. 1976 Mar;15(3):199–207. [PubMed] [Google Scholar]
- Stevens T. D., Watkins H. M. Rapid identification of viruses by indirect immunofluorescence: standardization and use of antiserum pool to nine respiratory viruses. Appl Microbiol. 1969 Mar;17(3):384–393. doi: 10.1128/am.17.3.384-393.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]