Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Sep;12(3):409–412. doi: 10.1128/jcm.12.3.409-412.1980

Chlamydial antigens stabilized with formalin for use in the micro-immunofluorescence test.

L Hanna, H Keshishyan
PMCID: PMC273598  PMID: 7012175

Abstract

Formalinized antigen suspensions prepared by differential centrifugation from crude infected yolk sacs and stored at 4 degrees C were satisfactory antigens during at least 36 weeks when used in chlamydial micro-immunofluorescence procedures.

Full text

PDF
409

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Briones O. C., Hanna L., Jawetz E., Dawson C. R., Ostler H. B. Type-specific antibodies in human Chlamydia trachomatis infections of the eye. J Immunol. 1974 Oct;113(4):1262–1270. [PubMed] [Google Scholar]
  2. Carr M. C., Hanna L., Jawetz E. Chlamydiae, cervicitis, and abnormal Papanicolaou smears. Obstet Gynecol. 1979 Jan;53(1):27–30. [PubMed] [Google Scholar]
  3. Hanna L., Jawetz E., Briones O., Ostler H. B., Keshishyan H., Dawson C. R. Antibodies to TRIC agents in matched human tears and sera. J Immunol. 1973 Jun;110(6):1464–1469. [PubMed] [Google Scholar]
  4. Hanna L., Jawetz E., Dawson C. R. Antibodies to two immunotypes of Chlamydia trachomatis in individuals with trachoma. Infect Immun. 1976 Aug;14(2):429–432. doi: 10.1128/iai.14.2.429-432.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hanna L., Jawetz E., Nabli B., Hoshiwara I., Ostler B., Dawson C. Titration and typing of serum antibodies in TRIC infections by immunofluorescence. J Immunol. 1972 Jan;108(1):102–107. [PubMed] [Google Scholar]
  6. Kuo C., Wang S., Grayston J. T. Differentiation of TRIC and LGV organisms based on enhancement of infectivity by DEAE-dextran in cell culture. J Infect Dis. 1972 Mar;125(3):313–317. doi: 10.1093/infdis/125.3.313. [DOI] [PubMed] [Google Scholar]
  7. McComb D. E., Nichols R. L. Antibodies to trachoma in eye secretions of Saudi Arab children. Am J Epidemiol. 1969 Oct;90(4):278–284. doi: 10.1093/oxfordjournals.aje.a121071. [DOI] [PubMed] [Google Scholar]
  8. McCormack W. M., Alpert S., McComb D. E., Nichols R. L., Semine D. Z., Zinner S. H. Fifteen-month follow-up study of women infected with Chlamydia trachomatis. N Engl J Med. 1979 Jan 18;300(3):123–125. doi: 10.1056/NEJM197901183000305. [DOI] [PubMed] [Google Scholar]
  9. REEVE P., TAVERNE J. A simple method for total particle counts of trachoma and inclusion blennorrhoea viruses. Nature. 1962 Sep 1;195:923–924. doi: 10.1038/195923a0. [DOI] [PubMed] [Google Scholar]
  10. Reeve P., Owen J., Oriel J. D. Laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract. J Clin Pathol. 1975 Nov;28(11):910–914. doi: 10.1136/jcp.28.11.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schachter J. Chlamydial infections (third of three parts). N Engl J Med. 1978 Mar 9;298(10):540–549. doi: 10.1056/NEJM197803092981005. [DOI] [PubMed] [Google Scholar]
  12. Schachter J., Cles L., Ray R., Hines P. A. Failure of serology in diagnosing chlamydial infections of the female genital tract. J Clin Microbiol. 1979 Nov;10(5):647–649. doi: 10.1128/jcm.10.5.647-649.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stirling P., Richmond S. The developmental cycle of Chlamydia trachomatis in McCoy cells treated with cytochalasin B. J Gen Microbiol. 1977 May;100(1):31–42. doi: 10.1099/00221287-100-1-31. [DOI] [PubMed] [Google Scholar]
  14. Treharne J. D., Darougar S., Simmons P. D., Thin R. N. Rapid diagnosis of chlamydial infection of the cervix. Br J Vener Dis. 1978 Dec;54(6):403–408. doi: 10.1136/sti.54.6.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wang S. P., Grayston J. T. Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol. 1970 Sep;70(3):367–374. doi: 10.1016/0002-9394(70)90096-6. [DOI] [PubMed] [Google Scholar]
  16. Wang S. P., Kuo C. C., Grayston J. T. A simplified method for immunological typing of trachoma-inclusion conjunctivitis-lymphogranuloma venereum organisms. Infect Immun. 1973 Mar;7(3):356–360. doi: 10.1128/iai.7.3.356-360.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wang S. P., Kuo C. C., Grayston J. T. Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test. J Clin Microbiol. 1979 Aug;10(2):259–261. doi: 10.1128/jcm.10.2.259-261.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wentworth B. B., Alexander E. R. Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells. Appl Microbiol. 1974 May;27(5):912–916. doi: 10.1128/am.27.5.912-916.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yong E. C., Chinn J. S., Caldwell H. D., Kuo C. C. Reticulate bodies as single antigen in Chlamydia trachomatis serology with microimmunofluorescence. J Clin Microbiol. 1979 Sep;10(3):351–356. doi: 10.1128/jcm.10.3.351-356.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES