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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Sep;12(3):451–454. doi: 10.1128/jcm.12.3.451-454.1980

Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor.

R B Johnson Jr, R Libby
PMCID: PMC273607  PMID: 7217336

Abstract

The proposed method was designed to replace the tedious and difficult separation of immunoglobulin M (IgM) from IgG by sucrose gradient sedimentation. In this method, a 250-microliter portion of serum diluted 20-fold was passed through a small column of quaternary aminoethyl-Sephadex A-50 ion exchanger. IgG was not retained, but additional washes were required to remove all but 5%. A second buffer-eluting fluid recovered an average of 80% of the original IgM in a defined dilution. The entire operation took 15 min. The efficiency of this process was evaluated by the following: (i) radial immunodiffusion measurements of IgG and IgM; (ii) recovery studies of isohemagglutinins; and (iii) demonstrated removal of interference by the rheumatoid factor. The method was applied successfully to distinguish rubella IgM antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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