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. 1980 Oct;12(4):509–516. doi: 10.1128/jcm.12.4.509-516.1980

Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria.

V Chester, T J Cleary
PMCID: PMC273626  PMID: 6999019

Abstract

The Minitek identification system (MT) was compared with a conventional testing battery for the characterization of 735 isolates which included 57 species and groups of nonfermentative (NF) and nonenteric fermentative (NEF) gram-negative bacteria. The MT correctly identified 585 of 616 NF (94,96%) and 115 of 119 NEF (96.65%) bacteria and 700 of 735 strains (95.24%) overall. A total of 31 NF and NEF (4.22%) bacteria were misidentified, and no identification was determined for four strains (0.69%). All strains of Acinetobacter anitratus, Pseudomonas maltophilia, P. fluroescens, and P. putida and all but one strain of P. aeruginosa were correctly identified. The most frequently misidentified taxa were CDC group Va-1, P. pickettii (Va-2), P. mendocina, and Moraxella urethralis (M-4). Supplemental tests were needed for the complete identification of 214 strains (29.11%). An average of 1.54 supplemental tests were used with each of these strains. A total of 134 strains (18.23%) had their identification delayed by 1 day due to supplemental testing. We recommend the use of the 42 degree C growth test with the MT. When used in accord with the manufacturer's instructions and with the MT code book the MT was found to be a valuable system for the identification of a wide variety of common and infrequently encountered NF and NEF bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnishan J., Ayers L. W. Rapid identification of nonfermentative gram-negative rods by the Corning N/F system. J Clin Microbiol. 1979 Feb;9(2):239–243. doi: 10.1128/jcm.9.2.239-243.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burdash N. M., Bannister E. R., Manos J. P., West M. E. A comparison of four commercial systems for the identification of nonfermentative gram-negative bacilli. Am J Clin Pathol. 1980 Apr;73(4):564–569. doi: 10.1093/ajcp/73.4.564. [DOI] [PubMed] [Google Scholar]
  3. Chester B. Semiquantitative catalase test as an aid in identification of oxidative and nonsaccharolytic gram-negative bacteria. J Clin Microbiol. 1979 Oct;10(4):525–528. doi: 10.1128/jcm.10.4.525-528.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dowda H. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, Gram-negative rods. J Clin Microbiol. 1977 Dec;6(6):605–609. doi: 10.1128/jcm.6.6.605-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farmer J. J., 3rd Vibrio ("Beneckea") vulnificus, the bacterium associated with sepsis, septicaemia, and the sea. Lancet. 1979 Oct 27;2(8148):903–903. doi: 10.1016/s0140-6736(79)92715-6. [DOI] [PubMed] [Google Scholar]
  6. Nadler H., George H., Barr J. Accuracy and reproducibility of the Oxi/Ferm system in identifying a select group of unusual gram-negative bacilli. J Clin Microbiol. 1979 Feb;9(2):180–185. doi: 10.1128/jcm.9.2.180-185.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oberhofer T. R. Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1979 Feb;9(2):220–226. doi: 10.1128/jcm.9.2.220-226.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oberhofer T. R., Rowen J. W., Cunningham G. F., Higbee J. W. Evaluation of the oxi/ferm tube system with selected Gram-negative bacteria. J Clin Microbiol. 1977 Dec;6(6):559–566. doi: 10.1128/jcm.6.6.559-566.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shayegani M., Lee A. M., McLynn D. M. Evaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli. J Clin Microbiol. 1978 Jun;7(6):533–538. doi: 10.1128/jcm.7.6.533-538.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shayegani M., Maupin P. S., McGlynn D. M. Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria. J Clin Microbiol. 1978 Jun;7(6):539–545. doi: 10.1128/jcm.7.6.539-545.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wellstood-Nuesse S. Comparison of the Minitek system with conventional methods for identification of nonfermentative and oxidase-positive fermentative gram-negative bacilli. J Clin Microbiol. 1979 Apr;9(4):511–516. doi: 10.1128/jcm.9.4.511-516.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. West M., Burdash N. M., Freimuth F. Simplified silver-plating stain for flagella. J Clin Microbiol. 1977 Oct;6(4):414–419. doi: 10.1128/jcm.6.4.414-419.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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